From macro to genes of the chorioalanthoid membrane of pregnant mares of equine clones

• Grant number: 20/10260-3
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2021
• End date: September 30, 2024
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Claudia Barbosa Fernandes
• Grantee: Claudia Barbosa Fernandes
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Alejandro Esteller-Vico ; Antônio Chaves de Assis Neto ; Bruno Cogliati ; Maria Augusta Alonso ; Mariana Matera Veras ; Perla Dagher Cassoli Fleury ; Pouya Dini
• Associated scholarship(s): 24/15214-0 - Histological evaluation of the umbilical cord of clone foals, BP.IC

Abstract

Cloning represents one of the major scientific breakthroughs in animal biotechnology, enabling not only animal production and reproduction, preservation of endangered species and production of cells and tissues to be for therapeutic usages. In equine species, cloning efficiency is considered low, around 0,1% to 15% of pregnancies result in viable foals. Studies demonstrate a diversity of abnormalities in plaentas from equine clones, such as inflammatory process and vascular alterations. Although the relevance of this biotecnhique, little is knownb on the causes of these alterations in chroriollantoic membrane, the implications in neonatal viability, and factors that affect the genome; endocrinological dynamic; cellular and tisseu structure of placentas from equine clones. Thus, the project will analyze histopathology, stereology, ultrastructure, steroidogenesis, epigenetic and transcriptomic in ten choriollantoic membranes from alive clone gestations, five from stillborn clone gestations (produced through the adult somatic cell nuclear transfer technique) and ten control gestations (produced through conventional Artificial Insemination and Embryo Transfer). Results obtained, when not descriptive, will be submitted to statistical analysis using SAS Institute (Cary, NC), where P d 0.05 is considered statistically significant. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VERSTRAETE, M. H.; CARNEIRO, F. P.; ORELLANA-GUERRERO, D.; DAELS, P.; FERNANDES, C. B.; DINI, P.. Transcriptomic analysis of the chorioallantois of equine cloned pregnancies. Journal of Equine Veterinary Science, v. 113, p. 1-pg., 2022-06-01. (20/10260-3)

CARNEIRO, FRANCIELI P.; ALONSO, MARIA A.; REDOAN, MARIANA A.; HENRIQUES, DYANA A.; LIMA, FABIO M. M.; OLIVEIRA, ROSANA; COGLIATI, BRUNO F.; CANISSO, IGOR B.; FERNANDES, CLAUDIA. First Confirmed Diagnosis of Nocardioform Placentitis ( Amycolatopsis lexingtonensis) in South America. Journal of Equine Veterinary Science, v. 110, p. 5-pg., 2022-03-01. (20/10260-3, 17/05425-0)

BOAKARI, YATTA LINHARES; LEGACKI, ERIN; ALONSO, MARIA AUGUSTA; FRANCISCO DOS SANTOS, ANA CAROLINA; NICHI, MARCILIO; CONLEY, ALAN J.; FERNANDES, CLAUDIA BARBOSA. Postnatal Dynamics of Circulating Steroid Hormones in Mule and Equine Neonates. VETERINARY SCIENCES, v. 9, n. 11, p. 10-pg., 2022-11-01. (20/10260-3, 17/05425-0)

TINEL, JULIA BOLDRINI; VERAS, MARIANA MATERA; WAKED, DUNIA; ALONSO, MARIA AUGUSTA; FERNANDES, CLAUDIA BARBOSA. Comparative stereological evaluation of the term allantochorion membrane in the mare pregnant with mule foals and equine foals. Animal Reproduction Science, v. 250, p. 9-pg., 2023-03-06. (20/10260-3)

ALONSO, MARIA AUGUSTA; BOAKARI, YATTA LINHARES; RICCIO, AMANDA VALLONE; BELLI, CARLA BARGI; FERNANDES, CLAUDIA BARBOSA. Perinatal parameters of mule and equine foals: Similarities and differences. JOURNAL OF VETERINARY BEHAVIOR-CLINICAL APPLICATIONS AND RESEARCH, v. 63, p. 5-pg., 2023-05-05. (20/10260-3)