Transitory history of obesity during different moments of life and its impact on allergy airway disease

• Grant number: 20/13940-5
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2021
• End date: October 31, 2023
• Field of knowledge: Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
• Principal Investigator: Gabriel Forato Anhê
• Grantee: Gabriel Forato Anhê
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• City of the host institution: Campinas
• Associated researchers: Marco Aurélio Ramirez Vinolo ; Yanira Riffo Vasquez

Abstract

Obesity is a highly prevalent condition that associates the positive energy balance with a chronic subclinical inflammatory state characterized by elevated levels of TNF± and IL-6 and increased infiltration of M1 macrophages in white adipose tissue. The exacerbation of the TH2 inflammatory response of allergic asthma is another characteristic observed in obese humans and mice. Some adaptations to obesity, such as hepatic steatosis and hyperleptinemia, have been described as persistent after weight loss. However, it is not known whether all inflammatory changes in obesity persist after weight loss. Thus, the aim of the present study is to determine whether the transient history of obesity at different times in life has a persistent effect on the exacerbation of the inflammatory response in an experimental model of allergic asthma in both genders. To achieve this goal, female and male C57BL/6 mice will be exposed to a transient history by HFD consumption (OB-Rev) or to permanent exposure to HFD (OB) or standard feed (CTL). The obesity episode in the OB-Rev groups will occur either during puberty or after puberty. These animals will be sensitized and challenged with chicken egg ovalbumin (OVA) to induce the allergic asthma model. As outcomes, we will analyze bronchoalveolar lavage (BAL) cells for the presence of eosinophils and neutrophils. BAL samples will be used to evaluate the levels of IL-4, IL-5, IL-10, IL-13, TGF-²1, TNF-± and IL-1². The serum will be used to assess IgE levels. The lung tissue will be evaluated for collagen deposition, leukocyte infiltration, mucus accumulation and expression of inflammatory pathway proteins. Lung function will be assessed in vivo with flexiVent equipment. The adipose tissue will be used to evaluate determinants of the obese phenotype (relative weight and expression of IL-6, leptin and adiponectin). (AU)

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

ANHE, GABRIEL FORATO; BORDIN, SILVANA. The adaptation of maternal energy metabolism to lactation and its underlying mechanisms. Molecular and Cellular Endocrinology, v. 553, p. 22-pg., 2022-06-11. (13/07607-8, 20/13940-5, 19/03196-0)

VICENTE, JULIA MODESTO; LESCANO, CAROLINE HONAISER; BORDIN, SILVANA; MONICA, FABIOLA ZAKIA; GOBBI, GABRIELLA; ANHE, GABRIEL FORATO. Agomelatine inhibits platelet aggregation through melatonin receptor-dependent and independent mechanisms. Life Sciences, v. 328, p. 14-pg., 2023-09-01. (19/03196-0, 13/07607-8, 19/19488-0, 20/13940-5)

ALMEIDA, LORENA DE SOUZA; TEIXEIRA, CAIO JORDAO; CAMPOS, CAROLINA VIEIRA; CASALOTI, LAIS GUADALUPE; SODRE, FRHANCIELLY SHIRLEY; CAPETINI, VINICIUS COOPER; AMARAL, ANDRESSA GODOY; PAYOLLA, TANYARA BALIANI; PANTALEAO, LUCAS CARMINATTI; ANHE, GABRIEL FORATO; et al. Low Birth Weight Intensifies Changes in Markers of Hepatocarcinogenesis Induced by Fructose Consumption in Rats. METABOLITES, v. 12, n. 10, p. 15-pg., 2022-10-01. (13/07607-8, 20/13940-5, 19/03196-0, 20/06397-3)