Regulatory proteins acting at the crossroads of light and jasmonate signaling: characterization of their molecular function and their impact on crop quality

• Grant number: 21/05071-0
• Support Opportunities: Regular Research Grants
• Start date: January 01, 2022
• End date: December 31, 2024
• Field of knowledge: Biological Sciences - Botany - Pant Physiology
• Agreement: Research Foundation - Flanders (FWO)
• Principal Investigator: Maria Magdalena Rossi
• Grantee: Maria Magdalena Rossi
• Principal researcher abroad: Alain Goossens
• Institution abroad: Vlaams Instituut voor Biotechnologie (VIB), Belgium
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Eduardo Purgatto ; Luciano Freschi
• Associated research grant(s): 23/01373-7 - Role of BBX proteins on JA signaling, AP.R
• Associated scholarship(s): 23/07259-1 - Regulatory proteins acting at the crossroads of light and jasmonate signaling: characterization of their molecular function and their impact on crop quality, BP.TT; 23/04146-1 - Regulatory proteins acting at the crossroads of light and jasmonate signaling: characterization of their molecular function and their impact on crop quality, BP.TT; 22/08238-5 - Characterization of regulatory proteins acting at the crossroads of light and jasmonate signaling and their impact on crop quality, BP.TT; 22/05210-2 - Functional characterization of tomato BBX proteins and their interactors, BP.PD

Abstract

Plant fitness is highly dependent on the ability to cope with multiple (a)biotic environmental cues, such as light and interacting organisms. Complex signaling networks driven by perception of these cues and involving endogenously produced phytohormones, program the appropriate cellular and organismal response and steer plant development and metabolism to promote fitness and survival. Often antagonistic inputs are provided, obliging plants to decide between growth and defense. Signaling by respectively light and the stress phytohormone jasmonate (JA), represents a crucial point of crosstalk between two such conflicting programs. Hence, increased in depth fundamental knowledge of the role and functioning of common players in light and JA signaling is highly desired, and ultimately may allow developing uncoupling strategies to improve plant yield and quality. In this project, we will employ cutting-edge protein-protein interactomics technologies to advance our understanding of crosstalk between light and JA signaling. We will specifically investigate transcription factor complexes that involve a limitedly investigated family of transcription factors, the BBX proteins, using tomato as a model. Unravelling the composition, dynamics, functioning and role of the BBX transcription factor complexes will allow revealing new mechanistic paradigms in JA-light crosstalk, and thereby advance our views on how traits, such as crop yield and nutritional quality are determined. (AU)