Investigation of the mechanisms involved in bone and tooth resorption due to root canal infection and dental trauma

• Grant number: 21/09272-0
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2022
• End date: February 29, 2024
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Francisco Wanderley Garcia de Paula e Silva
• Grantee: Francisco Wanderley Garcia de Paula e Silva
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• City of the host institution: Ribeirão Preto

Abstract

Microbial or traumatic dental injuries can result in pathological resorption of mineralized tissue (bone and tooth). This research proposal aims to investigate the mechanisms involved in two models of bone and dental resorption, resulting from root canal infection and from dental avulsion and replantation. To assess the mechanisms involved in the process of bone and dental resorption of microbial origin, rodents will be used and the induction of periapical lesion will be performed by exposure and contamination of root canals to the oral environment. To assess traumatic dental resorption, avulsion will be performed followed by immediate or delayed tooth replantation (after 60 minutes). Specifically, microtomographic and morphometric analysis will be performed under fluorescence microscopy to assess the area, size and volume of resorptions of mineralized tissues (bone tissue, dentin and cementum), histopathological analysis in hematoxylin and eosin, histoenzymology to detect the activity of the tartrate resistant acid phosphatase (TRAP) enzyme for evaluation of activity and identification of clastic cells and immunohistochemistry to confirm the inflammatory infiltrate in the periodontal ligament. The evaluation of the presence and location of active forms of matrix metalloproteinases in periapical tissues will be performed by means of in situ zymography. Data obtained will be submitted to statistical analysis according to their distribution. (AU)

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

GONCALVES, JULIANA DE LIMA; ALVES DUARTE, ANA CAROLINE; ALMEIDA-JUNIOR, LUCIANO APARECIDO; DE CARVALHO, FABRICIO KITAZONO; DE QUEIROZ, ALEXANDRA MUSSOLINO; MANFRIN ARNEZ, MAYA FERNANDA; FACCIOLI, LUCIA HELENA; GARCIA PAULA-SILVA, FRANCISCO WANDERLEY. Enamel biomineralization under the effects of indomethacin and celecoxib non-steroidal anti-inflammatory drugs. SCIENTIFIC REPORTS, v. 12, n. 1, p. 13-pg., 2022-09-22. (14/07125-6, 10/17611-4, 21/09272-0)

DE ALMEIDA-JUNIOR, LUCIANO APARECIDO; GONCALVES, JULIANA DE LIMA; ARNEZ, MAYA FERNANDA MANFRIN; JIMENEZ, NALLERY STEYSI ROSTRAN; MARANON-VASQUEZ, GUIDO ARTEMIO; DE PAULA-SILVA, FRANCISCO WANDERLEY GARCIA. Effects of Systemic and Local Anti-tumor Necrosis Factor-α Receptor-1 Administration on Periapical Bone Loss in Mice. JOURNAL OF ENDODONTICS, v. 51, n. 8, p. 6-pg., 2025-08-01. (21/09272-0)