Salivary biomarkers of COVID-19 and other viral respiratory infections

• Grant number: 21/07490-0
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2022
• End date: February 29, 2024
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Paulo Henrique Braz da Silva
• Grantee: Paulo Henrique Braz da Silva
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Bengt Olof Valter Hasséus ; Camila Malta Romano ; Giuseppe Palmisano ; Herculano da Silva Martinho ; José Angelo Lauletta Lindoso ; Keity Souza Santos ; Kelvin To ; Louise Zanella Martins ; Luiz Fernando Ferraz da Silva ; Simone Giannecchini ; Vanessa Salete de Paula
• Associated research grant(s): 22/08302-5 - The Latin America Optics and Photonics Conference (LAOP) 2022, AR.BR
• Associated scholarship(s): 23/11442-6 - Salivary biomarkers of COVID-19 and respiratory viral co-infections., BP.TT; 22/12145-2 - Salivary biomarkers of COVID-19 and respiratory viral co-infections, BP.TT

Abstract

COVID-19 is an emerging disease caused by the coronavirus SARS-CoV-2, which was first identified in China and then spread over other countries, thus being considered a pandemic by the World Health Organization (WHO). The disease is mainly characterised by fever, dry cough, myalgia and arthralgia, and may take a mild, moderate or severe form depending on the clinical involvement. Its diagnosis is mainly based on detecting viral RNA in nasopharyngeal specimens collected by swab. Saliva has been shown to be a less invasive alternative for detecting SARS-CoV-2, in addition to allowing the analysis of biomarkers which may be related to both diagnosis and prognosis of the disease. In this study project, we will evaluate the use of saliva for diagnosis and follow-up of COVID-19, as well as possible coinfections with other respiratory viruses. The saliva specimens will be used for evaluation not only of the immune-inflammatory response in patients diagnosed with COVID-19 regarding the mild, moderate and severe forms, but also of the viral excretion kinetics at different times by means of weekly collections until the outcome was achieved (i.e. hospital discharge or death). The immune-humoral response will be also evaluated by detecting IgM, IgG and IgA antibodies at the different experimental times. The metabolomic and proteomic profiles will be also determined by using vibrational spectroscopic techniques (Raman, SERS and FT-IR) and MALDI-TOF-MS. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE LUCA, ANA CLARA FALABELLO; MARINHO, GABRIELLA BUENO; FRANCO, JULIANA BERTOLDI; TENORIO, JEFFERSON DA ROCHA; ANDRADE, NATALIA SILVA; BATISTA, ALEXANDRE MENDES; MAMANA, ANA CAROLINA; TOZETTO-MENDOZA, TANIA REGINA; SAYANS, MARIO PEREZ; BRAZ-SILVA, PAULO HENRIQUE; et al. Quantification of Torque Teno Virus (TTV) in plasma and saliva of individuals with liver cirrhosis: a cross sectional study. FRONTIERS IN MEDICINE, v. 10, p. 7-pg., 2023-06-22. (21/00708-0, 17/18938-6, 21/07490-0)

LAZARI, LUCAS C.; ZERBINATI, RODRIGO M.; ROSA-FERNANDES, LIVIA; SANTIAGO, VERONICA FEIJOLI; ROSA, KLAISE F.; ANGELI, CLAUDIA B.; SCHWAB, GABRIELA; PALMIERI, MICHELLE; SARMENTO, DMITY J. S.; MARINHO, CLAUDIO R. F.; et al. ALDI-TOF mass spectrometry of saliva samples as a prognostic tool for COVID-1. OURNAL OF ORAL MICROBIOLOG, v. 14, n. 1, DEC 31 2022. (18/15549-1, 20/06409-1, 20/04923-0, 18/18257-1, 15/26722-8, 17/03966-4, 21/07490-0, 18/20468-0)

SCHWAB, GABRIELA; PALMIERI, MICHELLE; ZERBINATI, RODRIGO M.; SARMENTO, DMITRY J. S.; REIS, THAIS; ORTEGA, KAREM L.; KANO, ITALO T.; CAIXETA, RAFAEL A., V; HASSEUS, BENGT; SAPKOTA, DIPAK; et al. Lack of direct association between oral mucosal lesions and SARS-CoV-2 in a cohort of patients hospitalised with COVID-19. JOURNAL OF ORAL MICROBIOLOGY, v. 14, n. 1, p. 9-pg., 2022-12-31. (21/03004-3, 21/07490-0)

ZERBINATI, RODRIGO MELIM; PALMIERI, MICHELLE; SCHWAB, GABRIELA; FELIX, ALVINA CLARA; MARTINHO, HERCULANO; GIANNECCHINI, SIMONE; TO, KELVIN KAI-WANG; LINDOSO, JOSE ANGELO LAULETTA; ROMANO, CAMILA MALTA; BRAZ-SILVA, PAULO HENRIQUE. Use of saliva and RT-PCR screening for SARS-CoV-2 variants of concern: Surveillance and monitoring. Journal of Medical Virology, v. N/A, p. 4-pg., 2022-05-16. (19/03859-9, 21/07490-0)