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Biophotonics applied to study of extracellular vesicles as connectors of kidney-heart crosstalk


In the current stage of medicine, the clinical diagnosis of a patient is obtained through the analysis of clinical examinations, comparison with standard values of physiological and genetic parameters, high-precision medical images and detection of metabolites in biofluids such as blood or urine. However, even with a broad arsenal available for characterization and measurement of homeostasis, numerous cases of patients with similar symptoms and parameters, but affected by different diseases, are reported. Furthermore, the response of different individuals to the same treatment can be quite different. The establishment of a precise and personalized medical diagnosis and therapy (theranostic) considering the needs and response potential of each individual is one of the great challenges of modern medicine, also called by some precision medicine. Obtaining new biomarkers, characterizing their dynamic molecular response, and developing new therapeutic strategies, considering personalized molecular and genetic aspects, are possible strategies to overcome this challenge. In this project, inflammatory processes of systemic origin, such as cardiorenal syndrome (SCR), will be characterized by vibrational nano-spectroscopy ("Surface Enhancement Raman Spectroscopy" and "Photo-Induced Force Microscopy") as well as by conventional biological techniques and molecular and cellular of the systems involved. Type 3 SCR (SCR3) is characterized by acute kidney injury causing cardiac injury, and has been the subject of laboratory research over the past 10 years. Within the pathophysiology of SCR, extracellular vesicles (EVs) were identified as participating in the communication between kidneys and heart through nucleic acids and proteins. Given the above, this study aims to evaluate the participation of VEs produced by ischemic kidney injury in cardiac changes, using conventional techniques of cell/molecular biology and vibrational nanospectroscopy as a tool for the discovery of possible biomarkers. Thus, the unilateral renal ischemia and reperfusion (I/R) model will be performed for 60 minutes, followed by 8 days of renal reperfusion. Then, the vesicles present in the sera of ischemic and non-ischemic animals will be isolated and characterized using traditional techniques (flow cytometry and SEM), and biophotonics techniques. The main scientific and technological advances that we aim to achieve at the end of the project are: (i) identification of molecular signatures present in EVs after I/R and (ii) identification of new inflammatory biomarkers. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PIERETTI, JOANA CLAUDIO; FREIRE, BRUNA MOREIRA; ARMENTANO, GIOVANA MARCHINI; SANTANA, BIANCA DE MELO; BATISTA, BRUNO LEMOS; CARNEIRO-RAMOS, MARCELA SORELLI; SEABRA, AMEDEA BAROZZI. Chronic exposure to nitric oxide sensitizes prostate cancer cells and improved ZnO/CisPt NPs cytotoxicity and selectivity. International Journal of Pharmaceutics, v. 640, p. 8-pg., . (22/00321-0, 20/03646-2, 20/08566-7, 22/00153-0)
VERNIER, IMARA CARIDAD STABLE; NERES-SANTOS, RAQUEL SILVA; ANDRADE-OLIVEIRA, VINICIUS; CARNEIRO-RAMOS, MARCELA SORELLI. Immune Cells Are Differentially Modulated in the Heart and the Kidney during the Development of Cardiorenal Syndrome 3. CELLS, v. 12, n. 4, p. 17-pg., . (19/11077-0, 20/02923-2, 22/00153-0)

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