Continuous improvement of vaccines: Center for Viral Surveillance and Serological Assessment (CeVIVAS)

• Grant number: 21/11944-6
• Support Opportunities: Research Grants - Science Centers for Development
• Start date: June 01, 2022
• End date: May 31, 2027
• Field of knowledge: Health Sciences - Collective Health - Public Health
• Principal Investigator: Sandra Coccuzzo Sampaio Vessoni
• Grantee: Sandra Coccuzzo Sampaio Vessoni
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Pesquisadores principais: Daniel Carvalho Pimenta ; Eliana Faquim de Lima Mauro ; Geraldo Santana Magalhães ; Maria Carolina Quartim Barbosa Elias Sabbaga ; Simone Kashima Haddad
• Associated researchers: Alex Ranieri Jerônimo Lima ; Claudia Renata dos Santos Barros ; Daniel José Galafasse Lahr ; David Schlesinger ; Dimas Tadeu Covas ; Elaine Cristina Marqueze ; Evaldo Stanislau Affonso de Araujo ; Evandra Strazza Rodrigues Sandoval ; Heidge Fukumasu ; Hugo Aguirre Armelin ; Igor Cabral Corrêa ; Jayme Augusto de Souza-Neto ; Joao Paulo Fumio Whitaker Kitajima ; Jose Salvatore Leister Patane ; Jounghyeok Kwon ; Julia Pinheiro Chagas da Cunha ; Keith Crandall ; Luiz Carlos Junior Alcantara ; Luiz Lehmann Coutinho ; Maria Carolina Quartim Barbosa Elias Sabbaga ; Marta Giovanetti ; Maurício Lacerda Nogueira ; Nancy Starobinas ; Rejane Maria Tommasini Grotto ; Svetoslav Nanev Slavov ; Vincent Louis Viala
• Associated scholarship(s): 24/23798-2 - Implementation of a platform for analyzing the cellular immunity induced by immunizers produced by the Butantan Institute: protective cellular immunity in response to viral infections, BP.PD; 23/11919-7 - Identification and characterization of potential new circulating dengue virus lineages between 2021 and 2026: contributions to evolutionary monitoring, BP.DR; 24/03645-7 - Identification and sequencing of peptides derived from the hypervariable region of vaccination-induced antibodies, BP.PD; + associated scholarships 24/05506-4 - Continuous improvement of vaccines: Center for Viral Surveillance and Serological Assessment (CeVIVAS), BP.TT; 23/15913-3 - Bioinformatics in the phylogenetic, phylogeographic, and epidemiological analysis of the evolution of Influenza viruses in Brazil, BP.TT; 23/13992-3 - Implementation of a platform for analyzing the cellular immunity induced by immunizers produced by the Butantan Institute: Protective cellular immunity in response to viral infections, BP.PD; 23/14852-0 - Impact of different dengue genotypes on the pathophysiology and severity of the disease: a multi-omics approach to the disease and which biological factors lead to its worsening., BP.DR; 23/07688-0 - Comparative phylogenetics, phylogeography and epidemiology applied in understanding the evolution of COVID-19, Influenza, and Dengue viruses in Brazil, BP.PD; 23/08965-7 - Genomic surveillance of Influenza A and B viruses circulating in the city of São Paulo between 2023 and 2025, BP.DR; 23/11920-5 - Development of a pipeline for continuous improvement of monoclonal antibodies for therapeutic use in diseases caused by influenza and SARS-CoV-2., BP.PD; 23/11334-9 - Production of pseudoviral particles to evaluate neutralizing antibodies, BP.PD; 23/09546-8 - Development of Biosensor for Rapid Diagnosis of Dengue Serotypes, BP.TT; 22/16349-1 - Genomic surveillance and viral quasispecies reconstruction of dengue virus in an endemic region, BP.DR - associated scholarships

Abstract

Vaccination is the touchstone of public health policies. The history of vaccination shows that vaccines have been empirically developed; therefore, many studies are still needed for the continuous development of this biological product. The effectiveness of vaccines is evaluated through the reduction of the infection or illness among vaccinated individuals, and it is influenced by many factors, such as the individual's characteristics, demographic and immunological matters, and the circulating pathogens. Instituto Butantan evidenced its potential for rapid response during the COVID-19 pandemic, when its Scientific Development Center faced the challenges with technical adequacies and management training to integrate scientific and administrative sectors, such as the Municipal and State Health Departments. Based on this successful experience, this project aims to monitor some of these factors to obtain a continuous improvement of viral vaccines for Influenza, COVID-19, and Dengue. To that end, we begin with constant genomic surveillance of the circulating Influenza, SARS-CoV-2, and DENV viruses in the State of São Paulo, in the country, and neighboring countries. The data obtained will allow (i) studies on the evolutionary history of these viruses and (ii) knowing the viral variants circulating in the studied regions. To know the protective capacity of the vaccines used, we will investigate whether (i) sera from vaccinated individuals neutralize the infection of circulating variants and (ii) how is the cellular response of vaccinated individuals against the viral strain used in the immunization and against the variants circulating in the population. This work will allow the creation of a biobank of viral variants, a library of vaccinated individuals, and a bank of mononuclear cells isolated from the peripheral blood of vaccinated individuals that can also be used in future projects. In parallel, we will analyze the effectiveness of vaccines according to sociodemographic, health, and environmental data, comparing the effectiveness of vaccines according to viral variants. The results obtained will be continuously sent to bodies in the three administrative branches: municipal, state, and federal spheres, to contribute significantly to the decision-making of public policies. As a result, we will continuously provide data to the health system so it can face the challenges posed by the viruses studied herein with subsidies more rigorous than before. (AU)

Scientific publications

(19)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE ARAUJO, JULIANA CALABRIA; CARVALHO, ANA PAULA ASSAD; LEAL, CINTIA D.; NATIVIDADE, MANUELLE; BORIN, MARCUS; GUERRA, AUGUSTO; CAROBIN, NATALIA; SABINO, ADRIANO; ALMADA, MARIANA; COSTA, MARIA CRISTINA M.; et al. Detection of Multiple Human Viruses, including Mpox, Using a Wastewater Surveillance Approach in Brazil. PATHOGENS, v. 13, n. 7, p. 10-pg., 2024-07-01. (21/11944-6)

DE CAMPOS, GABRIEL MONTENEGRO; SANTOS, HAZERRAL DE OLIVEIRA; LIMA, ALEX RANIERI JERONIMO; LEITE, ANDERSON BRANDAO; RIBEIRO, GABRIELA; BERNARDINO, JARDELINA DE SOUZA TODAO; DO NASCIMENTO, JEAN PHELLIPE MARQUES; SOUZA, JULIANA VANESSA CAVALCANTE; DE LIMA, LOYZE PAOLA OLIVEIRA; LIMA, MARLON BRENO ZAMPIERI; et al. Unveiling viral pathogens in acute respiratory disease: Insights from viral metagenomics in patients from the State of Alagoas, Brazil. PLoS Neglected Tropical Diseases, v. 18, n. 9, p. 11-pg., 2024-09-01. (21/11944-6, 17/23205-8, 22/00910-6, 23/12155-0, 22/14958-0)

LIMA, MARLON BRENO ZAMPIERI; DANIEL, THUANY GIOVANA PEREIRA; BITENCOURT, HELLEN TAYANA OLIVEIRA; ALCANTARA, LUIZ CARLOS JUNIOR; HADDAD, RODRIGO; KASHIMA, SIMONE; ELIAS, MARIA CAROLINA; GIOVANETTI, MARTA; SAMPAIO, SANDRA COCCUZZO; SLAVOV, SVETOSLAV NANEV. Molecular frequency of human gemycircularvirus (GCYV) dna among blood donors from the Brazilian Amazon. TRANSFUSION CLINIQUE ET BIOLOGIQUE, v. 31, n. 2, p. 4-pg., 2024-04-17. (22/00910-6, 17/23205-8, 21/11944-6, 18/15826-5, 22/14958-0)

RODRIGUES, EVANDRA STRAZZA; SLAVOV, SVETOSLAV NANEV; DE LA ROQUE, DEBORA GLENDA LIMA; SANTOS, ELAINE VIEIRA; BORGES, JOSIANE SERRANO; EVARISTO, MARIANE; DA COSTA, PERICLES NATAN MENDES; DE MATOS MACONETTO, JULIANA; MARQUES, ADRIANA APARECIDA; BACCARIN, ANEMARIE DINARTE; et al. Epidemiology of the SARS-CoV-2 Omicron Variant Emergence in the Southeast Brazilian Population. MICROORGANISMS, v. 12, n. 3, p. 13-pg., 2024-03-01. (20/10127-1, 21/11944-6, 20/06441-2)

HILL, VERITY; CLEEMPUT, SARA; PEREIRA, JAMES SIQUEIRA; GIFFORD, ROBERT J.; FONSECA, VAGNER; TEGALLY, HOURIIYAH; BRITO, ANDERSON F.; RIBEIRO, GABRIELA; DE SOUZA, VINICIUS CARIUS; BRCKO, ISABELA CARVALHO; et al. A new lineage nomenclature to aid genomic surveillance of dengue virus. PLOS BIOLOGY, v. 22, n. 9, p. 27-pg., 2024-09-01. (18/14389-0, 21/11944-6)

LIMA, ALEX RANIERI JERONIMO; SANTOS, HAZERRAL DE OLIVEIRA; PEREIRA, JAMES SIQUEIRA; LEITE, ANDERSON BRANDAO; DO NASCIMENTO, JEAN PHELLIPE MARQUES; SOUZA, JULIANA VANESSA CAVALCANTE; LIMA, MARLON BRENO ZAMPIERI; DE ARAUJO, MYKAELLA ANDRADE; GIOVANETTI, MARTA; KALLAS, ESPER GEORGES; et al. Enterovirus D68 Subgenotype B3 Circulation in Children with Acute Respiratory Illness in the State of Alagoas, Brazil. Viruses-Basel, v. 17, n. 2, p. 8-pg., 2025-02-01. (21/11944-6, 17/23205-8, 23/11919-7)

DA SILVA, ANIELLY SARANA; DE CAMPOS, GABRIEL MONTENEGRO; ALTIZANI, GABRIELA MARENGONE; BARROS, ALICE CHAGAS; JUNQUEIRA, DENNIS MALETICH; KASHIMA, SIMONE; SAMPAIO, SANDRA COCCUZZO; ELIAS, MARIA CAROLINA; GIOVANETTI, MARTA; SCRIDELI, CARLOS ALBERTO; et al. Human pegivirus-1 (HPgV-1) RNA frequency and genotype distribution in pediatric oncology patients with febrile neutropenia. VIRUS RESEARCH, v. 350, p. 5-pg., 2024-10-11. (22/14958-0, 17/23205-8, 22/00910-6, 21/11944-6)

DE CAMPOS, GABRIEL MONTENEGRO; LIMA DE LA-ROQUE, DEBORA GLENDA; JERONIMO LIMA, ALEX RANIERI; ZUCHERATO, VICTORIA SIMIONATTO; DE CARVALHO, ENEAS; OLIVEIRA DE LIMA, LOYZE PAOLA; CATTONY NETO, PEDRO DE QUEIROZ; DOS SANTOS, MURILO MARCONI; CICCOZZI, MASSIMO; GIOVANETTI, MARTA; et al. Exploring Viral Metagenomics in Pediatric Patients with Acute Respiratory Infections: Unveiling Pathogens beyond SARS-CoV-2. MICROORGANISMS, v. 11, n. 11, p. 10-pg., 2023-11-01. (22/00910-6, 17/23205-8, 21/11944-6, 18/15826-5, 21/09446-8)

DE LA-ROQUE, DEBORA GLENDA LIMA; SANTOS, ELAINE VIEIRA; OLIVEIRA, RENATA APARECIDA MACHADO; SLAVOV, SVETOSLAV NANEV; RODRIGUES, EVANDRA S.; FONSECA, VAGNER; MARTINS, ANTONIO JORGE; GIOMO, DENISE BERGAMASCHI; TORRES, PAULA MARILIA AFONSO; GENTIL, DANIELLE CRISTINA DACANAL; et al. DENV-1 genotype V circulation during the nonepidemic period in the Northeast of Sao Paulo State endemic area. Journal of Medical Virology, v. 96, n. 3, p. 4-pg., 2024-03-01. (13/08135-2, 21/11944-6, 22/16349-1)

VAZQUEZ, CYNTHIA; ALCANTARA, LUIZ CARLOS JUNIOR; FONSECA, VAGNER; LIMA, MAURICIO; XAVIER, JOILSON; ADELINO, TALITA; FRITSCH, HEGGER; CASTRO, EMERSON; DE OLIVEIRA, CARLA; SCHUAB, GABRIEL; et al. Retrospective Spatio-Temporal Dynamics of Dengue Virus 1, 2 and 4 in Paraguay. Viruses-Basel, v. 15, n. 6, p. 14-pg., 2023-06-01. (21/11944-6)

CAVALCANTE SOUZA, JULIANA VANESSA; SANTOS, HAZERRAL DE OLIVEIRA; LEITE, ANDERSON BRANDAO; GIOVANETTI, MARTA; BEZERRA, RAFAEL DOS SANTOS; DE CARVALHO, ENEAS; TODAO BERNARDINO, JARDELINA DE SOUZA; VIALA, VINCENT LOUIS; HADDAD, RODRIGO; CICCOZZI, MASSIMO; et al. Viral Metagenomics for the Identification of Emerging Infections in Clinical Samples with Inconclusive Dengue, Zika, and Chikungunya Viral Amplification. Viruses-Basel, v. 14, n. 9, p. 8-pg., 2022-09-01. (17/23205-8, 18/15826-5, 21/09446-8, 21/11944-6, 20/13194-1, 19/08528-0)

DE LA ROQUE, DEBORA GLENDA LIMA; SANTOS, ELAINE VIEIRA; POLICASTRO, LUCCA ROCHA; EVARISTO, MARIANE; DA COSTA, PERICLES NATAN MENDES; EVARISTO, MARRIANE; YAMAMOTO, APARECIDA YULIE; GIOMO, DENISE BERGAMASCHI; TORRES, PAULA MARILIA AFONSO; GENTIL, DANIELLE CRISTINA DACANAL; et al. Exploring the Chikungunya virus landscape in a dengue-endemic Brazilian area. JOURNAL OF INFECTION AND PUBLIC HEALTH, v. 17, n. 7, p. 4-pg., 2024-05-30. (22/16349-1, 13/08135-2, 21/11944-6)

GIOVANETTI, MARTA; VAZQUEZ, CYNTHIA; LIMA, MAURICIO; CASTRO, EMERSON; ROJAS, ANALIA; FUENTE, ANDREA GOMEZ DE LA; AQUINO, CAROLINA; CANTERO, CESAR; FLEITAS, FATIMA; TORALES, JUAN; et al. Rapid Epidemic Expansion of Chikungunya Virus East/Central/ South African Lineage, Paraguay. Emerging Infectious Diseases, v. 29, n. 9, p. 5-pg., 2023-09-01. (21/11944-6)

SLAVOV, SVETOSLAV NANEV; LIMA DE LA-ROQUE, DEBORA GLENDA; MENDES DA COSTA, PERICLES NATAN; RODRIGUES, EVANDRA STRAZZA; SANTOS, ELAINE VIEIRA; BORGES, JOSIANE SERRANO; EVARISTO, MARIANE; MACONETTO, JULIANA DE MATOS; MARQUES, ADRIANA APARECIDA; MILHOMENS, JONATHAN; et al. Dynamics of SARS-CoV-2 Variants of Concern in Vaccination Model City in the State of Sao Paulo, Brazil. Viruses-Basel, v. 14, n. 10, p. 12-pg., 2022-10-01. (14/50947-7, 18/15826-5, 20/10127-1, 20/06441-2, 19/22155-2, 17/26950-6, 21/11944-6, 13/08135-2)

DE CAMPOS, GABRIEL MONTENEGRO; COSTA, THALITA CRISTINA DE MELLO; SILVEIRA, ROBERTA MARANINCHI; VALENCA, IAN NUNES; BEZERRA, RAFAEL DOS SANTOS; DARRIGO JUNIOR, LUIZ GUILHERME; VIEIRA, ANA CAROLINA DE JESUS; MESQUITA, CAMILA CAMPOS; LAURINDO, PATRICIA DA SILVA; CUNHA, RENATO GUERINO; et al. Viral Metagenomics in Patients Who Underwent Allogeneic Hematopoietic Stem Cell Transplantation (HSCT): A Brazilian Experience. MICROORGANISMS, v. 12, n. 12, p. 13-pg., 2024-12-01. (19/08528-0, 19/07861-8, 23/12155-0, 21/11944-6, 17/23205-8)

BANHO, CECILIA ARTICO; MARQUES, BEATRIZ DE CARVALHO; SACCHETTO, LIVIA; LIMA, ANA KAROLINE SEPEDRO; PARRA, MAISA CARLA PEREIRA; LIMA, ALEX RANIERI JERONIMO; RIBEIRO, GABRIELA; MARTINS, ANTONIO JORGE; BARROS, CLAUDIA RENATA DOS SANTOS; ELIAS, MARIA CAROLINA; et al. Dynamic clade transitions and the influence of vaccination on the spatiotemporal circulation of SARS-CoV-2 variants. NPJ VACCINES, v. 9, n. 1, p. 12-pg., 2024-08-10. (22/03645-1, 20/10127-1, 21/11944-6, 23/14670-0, 20/04836-0)

CRUVINEL, VANESSA RESENDE NOGUEIRA; DE CARVALHO, ENEAS; ALVES, DAIANI CRISTINA CILIAO; MARQUES, CARLA PINTAS; BEZERRA, RAFAEL DOS SANTOS; GIOVANETTI, MARTA; SAMPAIO, SANDRA COCCUZZO; ELIAS, MARIA CAROLINA; DE ARAUJO, WILDO NAVEGANTES; HADDAD, RODRIGO; et al. Unveiling microbial worlds: exploring viral metagenomics among waste pickers at Latin America's largest dumpsite. Revista do Instituto de Medicina Tropical de São Paulo, v. 66, p. 7-pg., 2024-01-01. (18/15826-5, 21/11944-6, 17/23205-8)

POLICASTRO, LUCCA ROCHA; LIMA DE LA-ROQUE, DEBORA GLENDA; CALADO, RODRIGO TOCANTINS; COVAS, DIMAS TADEU; ALCANTARA, LUIZ CARLOS; GIOVANETTI, MARTA; KASHIMA, SIMONE. Oropouche virus in Brazil: Assessing the risks and challenges for transfusion medicine. Vox Sanguinis, v. N/A, p. 4-pg., 2024-12-24. (13/08135-2, 21/11944-6, 22/16349-1)

BRCKO, ISABELA CARVALHO; DE SOUZA, VINICIUS CARIUS; RIBEIRO, GABRIELA; LIMA, ALEX RANIERI JERONIMO; MARTINS, ANTONIO JORGE; BARROS, CLAUDIA RENATA DOS SANTOS; DE CARVALHO, ENEAS; PEREIRA, JAMES SIQUEIRA; DE LIMA, LOYZE PAOLA OLIVEIRA; VIALA, VINCENT LOUIS; et al. Comprehensive molecular epidemiology of influenza viruses in Brazil: insights from a nationwide analysis. VIRUS EVOLUTION, v. 11, n. 1, p. 17-pg., 2025-01-08. (21/11944-6, 23/07688-0, 23/11920-5)