Project Zebratox: toxicological screening of larvicides of public and veterinary health importance

• Grant number: 22/03094-5
• Support Opportunities: Research Grants - Initial Project
• Start date: February 01, 2023
• End date: January 31, 2028
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Bruno Fiorelini Pereira
• Grantee: Bruno Fiorelini Pereira
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
• Associated researchers: Ana Carolina Luchiari ; Fábio Kummrow ; Gisele Giannocco ; Marília Cristina Oliveira Souza
• Associated research grant(s): 24/01418-3 - JOINT EFFECTS OF MICROPLASTIC AND INSECTICIDES APPLIED IN PUBLIC HEALTH CAMPAIGNS AND VETERINARY MEDICINE ON DIFFERENT STAGES OF DEVELOPMENT SPECIES DANIO RERIO (ZEBRAFISH)., AP.R
• Associated scholarship(s): 25/05728-0 - Analysis of endocrine disruption to chronic exposure to mixtures of the compounds Diflubenzuron and Pyriproxifen in the experimental model Danio rerio, BP.MS; 24/14045-0 - Analysis of biomarkers histopathological and behavioral of chronic exposure of mixtures of compounds Diflubenzuron and Piriproxifen in the Danio rerio experimental model, BP.MS; 23/09884-0 - Analysis of histopathological effects and endocrine disruption in chronic exposure to Diflubenzuron and Piriproxyfen in the experimental model Danio rerio, BP.DR

Abstract

Anthropogenic impacts in aquatic environments cause the exposure of fauna and flora to numerous substances, of which, many times, we are unaware of their real individual effects and in mixtures. Among the agents that are constantly released, pesticides are relevant, not only for the quantities used, but also for the diversity of compounds produced and registered in Brazil. Although some experts argue that there is no risk if the amount of active ingredients is within the regulated limit, data on the occurrence of pesticide active ingredients in Brazilian aquatic environments are still scarce. Among the ecotoxicological tests with fish, the zebrafish-based model has become popular for the evaluation of toxicity, mainly because it is an organism with similar anatomy, physiology and development to higher vertebrates and because it is representative and recognized as a model in aquatic environments. Therefore, this project aims to use several biomarkers to evaluate the effects of the active ingredients of the compounds Diclubenzuron (DBZ) and Pyriproxifen (PPF), two widely used pesticides that can pose risks to aquatic life and human health, focusing on their individual action and on mixtures. For this, this project will carry out analyzes aimed at identifying histopathological changes in the liver, gills and gonads, in addition to the possible action of these agents as endocrine disruptors, their impact on the behavior and embryonic development of the Danio rerio (Zebrafish) species. These analyzes bring a multidisciplinary character to the project and will allow the production of relevant data for the growth of knowledge of environmental toxicology and the identification of how such substances in mixture can interact with the biota, aiming at the conservation of species and the generation of data that help the reformulation and updating of Brazilian legislation. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TOMINAGA, FLAVIO KIYOSHI; BRITO, RAFAELLA SILVA; DOS SANTOS, ISIS WANESSA FERREIRA; VALLE, AMANDA DE AZEVEDO; GIANNOCCO, GISELE; MACIEL, RUI MONTEIRO DE BARROS; KUMMROW, FABIO; SOUZA, MARILIA CRISTINA OLIVEIRA; LUCHIARI, ANA CAROLINA; PEREIRA, BRUNO FIORELINI. Microplastic Interference with Fipronil Toxicity to Zebrafish Embryonic Development. MICROPLASTICS, v. 3, n. 4, p. 13-pg., 2024-12-01. (23/09884-0, 24/01418-3, 22/03094-5, 24/10993-1, 23/15540-2)

TEIXEIRA, JULIA ROBERT DE SOUSA; DE SOUZA, AUGUSTO MONTEIRO; DE MACEDO-SAMPAIO, JOAO VITOR; TAVARES, LIVIA ALVES DE MACEDO; PEREIRA, BRUNO FIORELINI; DE MEDEIROS, SILVIA REGINA BATISTUZZO; LUCHIARI, ANA CAROLINA. Chronic exposure to low concentration of diflubenzuron and pyriproxyfen induces brain oxidative stress and inflammation and alters locomotion in zebrafish (Danio rerio). Environmental Research, v. 264, p. 11-pg., 2025-01-01. (22/03094-5)

TOMINAGA, FLAVIO KIYOSHI; BRITO, RAFAELLA SILVA; DO NASCIMENTO, JULIANA OLIVEIRA; GIANNOCCO, GISELE; MACIEL, RUI MONTEIRO DE BARROS; KUMMROW, FABIO; PEREIRA, BRUNO FIORELINI. Pyriproxyfen toxicity to fish and crustaceans: A literature review. Environmental Research, v. 274, p. 20-pg., 2025-03-07. (23/09884-0, 24/01418-3, 24/10993-1, 22/03094-5)