Biology of Bacteria and Bacteriophages Research Center

• Grant number: 21/10577-0
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• Field of knowledge: Biological Sciences - Biochemistry - Biochemistry of Microorganisms
• Principal Investigator: Shaker Chuck Farah
• Grantee: Shaker Chuck Farah
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Pesquisadores principais: Aline Maria da Silva ; Beny Spira ; Cristiane Rodrigues Guzzo Carvalho ; Cristina Elisa Alvarez Martinez ; Henrique Ferreira ; João Carlos Setubal ; Marcelo Brocchi ; Marcio Vinicius Bertacine Dias ; Marilis Do Valle Marques ; Regina Lúcia Baldini ; Roberto Kopke Salinas ; Rodrigo da Silva Galhardo ; Sandro Roberto Marana
• Associated researchers: Cristiane Rodrigues Guzzo Carvalho ; Cristina Elisa Alvarez Martinez ; Frederico José Gueiros Filho ; Germán Gustavo Sgro ; João Carlos Setubal ; José Freire da Silva Neto ; Leonardo Talachia Rosa ; Marcio Vinicius Bertacine Dias ; Marco Aurélio Takita ; Rita de Cássia Café Ferreira ; Robson Francisco de Souza ; Rodrigo da Silva Galhardo
• Associated research grant(s): 24/09645-9 - Integration of Experimental and in silico Methods in the Study of Antimicrobials Mechanisms Resistance in Mycobacterium tuberculosis, AP.R SPRINT; 24/02235-0 - Study of the NlpC_P60-like enzymes for the development of novel antimycobacterial agents, AP.R; 23/15471-0 - Multi-user equipment approved in grant 2021/10577-0: BMG Labtech Clariostar multimode microplate reader, AP.EMU; 23/12177-4 - Multi-user equipment approved in grant 2021/10577-0: circular dichroism spectrophotometers JASCO, model J-1500, AP.EMU; 23/12239-0 - Multi-user equipment approved in grant 2021/10577-0: PEAQ-ITC (Isothermal Calorimeter), AP.EMU
• Associated scholarship(s): 24/04962-6 - Inhibition of dihydrofolate reductase from Mycobacterium tuberculosis and pathogenic fungi by antimicrobials identified by Fragment-Based Drug Discovery, BP.DR; 24/10763-6 - Structural study by electron cryomicroscopy of the MotA/MotB flagellar stator of Leptospira interrogans, BP.DD; 24/15711-4 - Identification of compounds that interact with the ClpC1 enzyme of the ClpP1P2C1 protein complex of Mycobacterium tuberculosis by fragment screening, BP.IC; + associated scholarships 24/19817-1 - Study of the mechanism of action and resistance to pyrazinamide in Mycobacterium tuberculosis, BP.IC; 24/12387-1 - Development of a system for the identification and characterization of peptides associated with the adsorption of Xanthomonas citri bacteriophages, BP.DR; 24/01729-9 - Development and application of a computational tool for surveys of bacterial species using metagenomics datasets, BP.DD; 24/14262-1 - Public outreach and science communication of CEPID B3, BP.JC; 24/13851-3 - Evaluation of phage depolymerases against Xanthomonas citri subsp. citri, BP.IC; 24/10539-9 - Characterization of the nucleoprotein complex interconnecting the divisome and the terminal replication region of the Xanthomonas citri chromosome, BP.DR; 24/16116-2 - Screening for molecules that inhibit widespread bacterial transcription factors, BP.IC; 24/09014-9 - STUDY OF THE REGULATION OF EXPRESSION OF ACONITASE AND THE EFFECT OF ITS LIGATION TO THE RNA DEGRADOSOME IN CAULOBACTER CRESCENTUS, BP.MS; 24/12945-4 - Expanding the host range of lytic bacteriophages through recombination and selection, BP.DR; 23/03213-7 - Salmonella enterica and Membrane Vesicles (MVs) in Vaccine Development, BP.DR; 24/03230-1 - Characterization of FAN1 DNA repair pathway in Pseudomonas aeruginosa, BP.DD; 23/08535-2 - Construction of "Artilysins" for the control of Xanthomonas citri subsp. citri, BP.PD; 23/16089-2 - Cryo-EM study of the adsorption of a type IV pilus-specific bacteriophage to its receptor, BP.PD; 24/06039-0 - Exploring biotechnological applications of bacteria-killing type IV secretion systems., BP.PD; 24/05782-1 - Implementation of bioinformatic tools and pipelines for analyses of phage and bacterial genomes and their interactions., BP.TT; 23/17618-9 - Xanthomonas citri R-bodies: expression and structural characterization, BP.IC; 24/01290-7 - Characterization of the stress response by alternative sigma factors in Xanthomonas citri, BP.PD; 24/03640-5 - Intersection between stress responses in bacteria, BP.PD; 24/03217-5 - Structural studies of the inner membrane subunits of the Xanthomonas citri Type IV Secretion System using NMR spectroscopy and cryoelectron microscopy, BP.PD; 24/01159-8 - Uncovering bacterial-phage interplay by monitoring transcriptomic changes during phage infection., BP.MS; 24/03134-2 - A novel domain and the new signaling pathway of DgcP in Pseudomonas aeruginosa, BP.DD; 23/13894-1 - CryoEM study of the adsorption of a phage to a type IV pilus, BP.DD; 24/02648-2 - Development and implementation of the educational project MicroMath, BP.IC; 23/13202-2 - Characterization of Xanthomas citri bacteriophage, BP.IC; 23/13291-5 - Determining the intrinsic levels of ppGpp across a species and its influence on growth rate and tolerance to antibiotics, BP.DD; 23/05679-3 - Characterization of xac4136 gene as a putative Type VI Secretion System effector in Xanthomonas citri, BP.MS; 23/14021-1 - Phage endolysins (ELs) as antimicrobial agents to Gram-negative bacteria, BP.PD; 23/12063-9 - Dissemination of concepts in microbiology: Impact assessment on basic education., BP.IC; 23/09812-0 - Study of the action of Pseudomonas aeruginosa secretion systems and their effectors in co-cultures with Aspergillus fumigatus, BP.IC; 23/10731-4 - Implementation of computational infrastructure for NMR spectroscopy, BP.TT; 23/11219-5 - Development and implementation of the educational project MicroMath, BP.IC; 23/06833-6 - Evaluating the role of aconitase in the regulation of FeS synthesis genes in Caulobacter, BP.IC; 23/06554-0 - Identification of compounds that interact with DNA gyrase from Mycobacterium tuberculosis through fragment screening, BP.IC; 23/06729-4 - Training of Pseudomonas aeruginosa lytic bacteriophages to overcome bacterial resistance, BP.IC; 23/06724-2 - Isolation of Pseudomonas aeruginosa lytic bacteriophages from various natural sources, BP.IC; 17/23627-0 - Determination of the structural bases of Biosynthetic formation of heterocyclic rings in ionophores polyethers, BP.PD - associated scholarships

Abstract

The CEPID B3 is a network with research hubs based in São Paulo (IQ-USP, ICB-USP), Campinas (IB-UNICAMP, IAC), Ribeirão Preto (FCFRP-USP, FMRP-USP) and Rio Claro (UNESP) that will collaborate to carry out cutting edge basic research into molecular mechanisms that are of prime importance to understand bacterial reproduction, multicellular behavior, competition, colonization and interactions with their eukaryotic hosts and bacteriophages. The acquired knowledge will be used in the search and development of new chemical and biological inhibitors of bacterial growth. (AU)