The Antimicrobial Resistance Institute of São Paulo (The Aries Project)

• Grant number: 21/10599-3
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• Start date: May 01, 2023
• End date: April 30, 2028
• Field of knowledge: Health Sciences - Collective Health - Public Health
• Principal Investigator: Arnaldo Lopes Colombo
• Grantee: Arnaldo Lopes Colombo
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Pesquisadores principais:( Atuais ): Ana Cristina Gales ; Elbert Einstein Nehrer Macau ; Elize Massard da Fonseca ; Moacir de Miranda Oliveira Junior ; Nilton Erbet Lincopan Huenuman ; Sergio Schenkman
• Pesquisadores principais:( Anteriores ): Adriano Massuda
• Associated researchers: Ademar Arthur Chioro dos Reis ; Aline Mariane de Faria ; Anete Pereira de Souza ; Antonio Carlos Campos Pignatari ; Camila Bertini Martins ; Carlos Magno Castelo Branco Fortaleza ; Carlos Pelleschi Taborda ; Carlos Roberto Veiga Kiffer ; Cesar Rossas Mota Filho ; Christiane de Arruda Rodrigues ; Cláudia Sofia Frias Pinto ; Daielly Melina Nassif Mantovani ; Daniela Goncales Galasse Rando ; Daniela Leal Musa ; Décio Luis Semensatto Junior ; Elize Massard da Fonseca ; Fabio Augusto Faria ; Felipe Mendes Borini ; Flávia Cristina Martins Queiroz Mariano ; Gabriela de Brelaz ; Geórgia Christina Labuto Araújo ; Gustavo Henrique Goldman ; Iran Malavazi ; João Nóbrega de Almeida Júnior ; Katherine Keenan ; Leandro Augusto Calixto ; Lilian Berton ; Luiz Sidney Longo Junior ; Marcelo Pillonetto ; Marcos Gonçalves Quiles ; Marina Farrel Côrtes ; Mirian Chieko Shinzato ; Patricia Sartorelli ; Rodrigo Cayô da Silva ; Simone Georges El Khouri Miraglia ; Suzan Pantaroto de Vasconcellos ; Wagner Luiz Batista ; Welington Luiz de Araújo
• Associated research grant(s): 24/15193-3 - Multi-user equipment approved in grant 2021/10599-3 equipment name high-resolution accurate mass spectrometer Orbitrap: Orbitral Exploris 120 -, Thermo Scientific, AP.EMU; 24/10656-5 - Multi-user equipment approved in grant 2021/10599-3 equipment name mass spectrometer type Malditime-of-Flight_Bruker Biotyper® (Sirius IVD System), AP.EMU
• Associated scholarship(s): 24/20740-3 - Microbiological and genomic surveillance of critical priority pathogens in non-human primates, BP.MS; 24/19958-4 - Establishing an Epidemiological Surveillance Network for Antibiotic-Resistant Bacteria in Veterinary Teaching Hospitals across Brazil, BP.PD; 24/19960-9 - BreathBr: Exploring the fingerprints of volatile organic compounds (VOCs) as diagnostic biomarkers of lung infections in immunocompromised patients, BP.PD; + associated scholarships 24/18634-0 - Phenotypic and Genetic Characterization of Virulence and Escape Mechanisms of Candida parapsilosis in Four Hospital Outbreaks in Brazil, BP.PD; 24/19523-8 - The development of advanced solutions to address the Antimicrobial resistance: Cutting-edge innovations and business opportunities for healthcare researchers., BP.PD; 24/20007-4 - Modeling the burden of disease of Infections caused by resistant microorganisms, BP.PD; 24/19006-3 - Genome-wide association studies of critical priority pathogens integrated to One Health in Brazil, BP.PD; 24/18638-6 - Investigation of metabolic alterations in ESKAPE microorganisms and infected patients using metabolomic approaches, and the discovery of bioactives from endophytic fungi for the prevention of nosocomial infections, BP.PD; 24/18733-9 - Metabolomics Applied to Novel Strategies for Antimicrobial Resistance Control and Treatment, BP.PD; 24/10063-4 - Establish the ARIES Advanced Diffusion Center, in addition to the areas of creation and Education, BP.JC; 24/08820-1 - Analysis of technological maturity in antimicrobial resistance research: case study at the Antimicrobial Resistance Institute of São Paulo (CEPID-ARIES), BP.DR; 24/07461-8 - Nanotherapy for controlling transmission and systemic infection by Candida auris: in vitro and in vivo studies, BP.PD; 24/15586-5 - Evaluation of cefiderocol activity against Gram-negative bacilli isolated from a university hospital in the city of São Paulo, BP.MS; 24/13125-0 - Patterns Changes in the Occurrence of Microbial Infections in Brazil, BP.MS; 24/14053-3 - Evaluation of Different Techniques for Antimicrobial Susceptibility Tests of Cefiderocol Against Enterobacterales Isolated from Brazilian Hospitals, BP.IC; 24/13900-4 - Development of a Databse for Bacteria and Phages, BP.IC; 24/13899-6 - Development of a Database for Bacteria and Phages, BP.IC; 24/08865-5 - Photoelectrodegradation of ciprofloxacin using Ti-O-Nb nanotubular oxides in varied compositions as photocatalyst, BP.IC; 24/00854-4 - Targeting antimicrobial resistance genes using phage-delivered CRISPR- Cas, BP.PD; 24/08937-6 - Establishment of a technique using CRISPR to detect microorganisms in veterinary samples, BP.DR; 24/09157-4 - Investigating the role of non-antibiotic pollutants on antifungal resistance, BP.DR; 24/06693-2 - ETL Process for SIHSUS Data in a DataLake House, BP.IC; 24/07312-2 - Adsorption of antifungals on polymers abundant in aquatic environments and hospital materials and their contribution to Candida auris resistance, BP.MS; 24/03612-1 - Evaluation of the role of the two-component system in the cell signaling of echinocandin resistant Candida glabrata, BP.PD; 24/05704-0 - Investigating the role of non-antibiotic pollutants on antifungal resistance, BP.DR; 24/05998-4 - Collection and Curation of Public Health Data Related to the Problem of Microbial Resistance, BP.IC; 24/06593-8 - Isolation and identification of antimicrobial-resistant bacteria obtained from the environment, BP.IC; 24/03154-3 - Inactivation of Vancomycin Resistance Genes in Enterococcus spp. Using CRISPR/Cas, BP.DR; 24/02476-7 - Exploring different statistical approaches to assess the relationship between microbial resistance and climate change in Brazil., BP.PD; 24/02579-0 - Estimation of the Burden of Disease of Infections caused by Resistant Microbials in Brazil, BP.PD; 23/18292-0 - BiocinBR: Bacteriocin research platform to combat pathogens resistant to critical priority antimicrobials in Brazil, BP.DR; 24/00892-3 - Investigation of the involvement of the VPS51 protein in the late complex and its role in drug resistance in Candida tropicalis, BP.DR; 23/16216-4 - Genomic investigation of probiotics/bacteriocins for the treatment of antimicrobial-resistant pathogens, BP.PD; 23/15368-5 - Frequency of pathogenic fungi isolates in recreational coastal water from the state of São Paulo (Pillar 2 - Project 4 - Work Package 5), BP.IC; 23/16522-8 - Evaluation of the role of soil as a reservoir and dissemination of pathogenic fungi in surrounding areas of the main hospitals in the city of São Paulo, BP.IC; 23/14561-6 - Differences among healthy, sick and MDR colonized patient's gut microbiome and the effect of Fecal Microbiota Transplantation as a therapeutic strategy to restore the gut microbiome., BP.PD; 23/10416-1 - Use of the CRISPR-Cas12 methodology for the diagnosis of infectious bacteria in a hospital environment, BP.DR - associated scholarships

Abstract

Antimicrobial resistance (AMR) has been considered one of the main threats to the human race in the 21st century. We are facing an unstoppable fast-forward instance of Darwinian adaptation through natural selection, in which microbes are constantly evolving to survive antimicrobials. As part of their natural behavior, they adapt continuously. They will succeed unless we develop effective approaches to control and prevent further AMR spread. We propose to establish the Antimicrobial Resistance Institute of São Paulo (ARIES) that will join researchers from 5 universities in São Paulo with experts from public organizations (EMBRAPA, CETESB) and representatives of the Ministry of Agriculture, Livestock and Food Supply, Ministry of Health, Brazilian Health Regulatory Agency (ANVISA) and Pan American Health Organization (PAHO). Working together in a pioneering enterprise, they will fight against AMR implementing the concepts of Global Health and One Health. Mitigation of the AMR threat requires a multidisciplinary approach that recognizes and interferes in the drivers of AMR in the human-animal-environment interface. ARIES will count on five main pillars to achieve our key objectives: 1) Socio-Environmental Observatory and Data Science Center: surveillance of ecosystems involved in the emergence and spread of AMR; 2) Characterization of microbial evolution, mechanisms of AMR and microorganism fitness and pathogenicity; 3) Development and validation of new strategies for control and prevention of AMR in humans, animals, and environment; 4) Translation of the successful initiatives to Business and Public Health Policies; and 5) Diffusion of ARIES - Knowledge in connection with Society, Education and Dissemination. ARIES will not only contribute to elaborate a more sophisticated and comprehensive surveillance system able to early detect the emergence of AMR in the context of One Health, but also generate new strategies to respond, contain, and prevent the resistant infections across healthcare settings, food, and communities. (AU)

Scientific publications

(12)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SPRUIJTENBURG, BRAM; DE ALMEIDA JUNIOR, JOAO NOBREGA; RIBEIRO, FELIPE DE CAMARGO; KEMMERICH, KAROLINE KRISTINA; BAETA, KARLA; MEIJER, EELCO F. J.; DE GROOT, THEUN; MEIS, JACQUES F.; COLOMBO, ARNALDO LOPES. Multicenter Candida auris outbreak caused by azole-susceptible clade IV in Pernambuco, Brazil. MYCOSES, v. 67, n. 6, p. 8-pg., 2024-06-01. (21/10599-3)

GUIMARAES, TACIANA G. S.; BARROS, LUCELIA A.; SILVA, RICARDO SANTOS; GONZALEZ, MARIO H.; CARRILHO, ELMA N. V. M.; LABUTO, GEORGIA. Synthesis and characterization of biochars modified with natural deep eutectic solvent (NADES) for dipyrone removal from aqueous medium. SUSTAINABLE CHEMISTRY AND PHARMACY, v. 35, p. 18-pg., 2023-10-01. (22/06823-8, 21/06471-1, 21/14759-5, 21/14791-6, 20/12263-0, 21/10599-3, 20/14419-7, 22/08358-0)

FRANCISCO, ELAINE C.; DESNOS-OLLIVIER, MARIE; DIELEMAN, CHENDO; BOEKHOUT, TEUN; SANTOS, DANIEL WAGNER DE C. L.; MEDINA-PESTANA, JOSE O.; COLOMBO, ARNALDO L.; HAGEN, FERRY. Unveiling Trichosporon austroamericanum sp. nov.: A Novel Emerging Opportunistic Basidiomycetous Yeast Species. Mycopathologia, v. 189, n. 3, p. 14-pg., 2024-06-01. (17/02203-7, 19/24960-0, 21/10599-3)

COLOMBO, ARNALDO L.; PECANHA-PIETROBOM, PAULA M.; SANTOS, DANIEL WAGNER DE C. L.; CACERES, DIEGO H.. When to suspect and how properly early detect and treat patients with endemic mycoses. MOLECULAR ASPECTS OF MEDICINE, v. 102, p. 24-pg., 2025-04-01. (21/10599-3)

RIBEIRO, FELIPE DE CAMARGO; KEMMERICH, KAROLINE KRISTINA; GONCALE, JULIANA CAPARROZ; JUNQUEIRA, JULIANA CAMPOS; MANNAN, MOHAMMAD; NABEELA, SUNNA; COLOMBO, ARNALDO LOPES; UPPULURI, PRIYA. Candida albicans Recovered From Persistent Candidemia Exhibits Enhanced Virulence Traits. Journal of Infectious Diseases, v. N/A, p. 10-pg., 2025-01-21. (21/00527-5, 22/01975-4, 17/02203-7, 21/10599-3)

BARNA, BIANCA; CALDAS, LHAIS ARAUJO; MONTEIRO, JACKSON; DOS SANTOS, AUGUSTO LEONARDO; PASCON, RENATA CASTIGLIONI; VALLIM, MARCELO AFONSO; FERREIRA, MARCELO JOSE PENA; GONCALVES, SARAH SANTOS; DOS SANTOS, GLAUCIA QUEIROZ; RODRIGUES, ANDERSON MESSIAS; et al. Endophytic Fungi of Calea pinnatifida (Asteraceae): Dereplication of Crude Extracts, Antimicrobial Properties, and Identification of New Tetronic Acid Derivative Produced by Hypomontagnella barbarensis. JOURNAL OF FUNGI, v. 11, n. 1, p. 18-pg., 2025-01-01. (22/09202-4, 20/16554-9, 21/10599-3)

DIEHL, CAMILA; PINZAN, CAMILA FIGUEIREDO; DE CASTRO, PATRICIA ALVES; DELBAJE, ENDREWS; CARNERO, LAURA C. GARCIA; SANCHEZ-LEON, EDDY; BHALLA, KABIR; KRONSTAD, JAMES W.; KIM, DONG-GYU; DOERING, TAMARA L.; et al. Brilacidin, a novel antifungal agent against Cryptococcus neoformans. MBIO, v. 15, n. 7, p. 28-pg., 2024-06-25. (22/13603-4, 22/08796-8, 21/10599-3, 21/04977-5)

HAMBURGER, FLAVIO GUINSBURG; GALES, ANA CRISTINA; COLOMBO, ARNALDO LOPES. Systematic Review of Candidemia in Brazil: Unlocking Historical Trends and Challenges in Conducting Surveys in Middle-Income Countries. Mycopathologia, v. 189, n. 4, p. 12-pg., 2024-08-01. (21/10599-3)

MOSES, IKECHUKWU BENJAMIN; SANTOS, INGRID NAYARA MARCELINO; RIBEIRO, AGHATA CARDOSO DA SILVA; SANTOS, FERNANDA FERNANDES; CAYO, RODRIGO; GALES, ANA CRISTINA. Co-resistance to methicillin and clindamycin among coagulase-negative staphylococci isolates recovered from pet food in Brazil. International Journal of Food Microbiology, v. 418, p. 7-pg., 2024-05-04. (21/10599-3)

MOSES, IKECHUKWU BENJAMIN; RIBEIRO, AGHATA CARDOSO DA SILVA; VALIATTI, TIAGO BARCELOS; SANTOS, FERNANDA FERNANDES; CAYO, RODRIGO; GALES, ANA CRISTINA. First Detection of International High-Risk bla_KPC-2-Harbouring Escherichia coli Pandemic Lineage ST648 in Pet Food Packages. TRANSBOUNDARY AND EMERGING DISEASES, v. 2024, p. 8-pg., 2024-03-05. (21/10599-3)

NUNES RIBEIRO, TAINARA APARECIDA; DE PAULA, DAIANE DULCILEIA MORAES; BORGES, MARCELLA MATOS CORDEIRO; CALIXTO, LEANDRO AUGUSTO; BORGES, KEYLLER BASTOS. Sample Preparation Approaches for Determination of Quinolones in Aqueous Matrixes: Systematic Review. ACS MEASUREMENT SCIENCE AU, v. N/A, p. 12-pg., 2024-11-14. (21/10599-3)

REIS, ADRIANE S.; DOS SANTOS, LAURITA; CUNHA JR, AMERICO; KONSTANTYNER, THAIS C. R. O.; MACAU, ELBERT E. N.. Unravelling COVID-19 waves in Rio de Janeiro city: Qualitative insights from nonlinear dynamic analysis. INFECTIOUS DISEASE MODELLING, v. 9, n. 2, p. 15-pg., 2024-06-01. (21/10599-3)