Antimicrobial and osteogenic surface development by electrodeposition of polypyrrole film on titanium surfaces treated by plasma electrolytic oxidation: a multifunctional coating for dental implants

• Grant number: 22/16267-5
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2023
• End date: September 30, 2025
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Valentim Adelino Ricardo Barão
• Grantee: Valentim Adelino Ricardo Barão
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Associated researchers: Magda Feres Figueiredo ; Nilson Cristino da Cruz
• Associated research grant(s): 24/02209-9 - Development of a Three-dimensional Smart Electroconductive Hydrogel as an Advanced Strategy for Peri-implant Infection Control, AP.R

Abstract

The surface coating of biomaterials by conductive polymers, such as polypyrrole (PPy), demonstrates to be a promising strategy due to its unique electrical properties. Our previous study showed that the pretreatment of titanium (Ti) surfaces by Plasma Electrolytic Oxidation (PEO) followed by the deposition of the PPy film (PEO+PPy), improved the mechanical and electrochemical properties of Ti. However, considering that the main implant failures are related to polymicrobial infection induced by biofilms and the deficiency in the osseointegration process, other features of Ti also need to be enhanced. Therefore, this study aims to develop a PEO+PPy coating with antibacterial properties through the zinc oxide (ZnO) incorporation (by electrodeposition) and to promote a surface with osteogenic activity through the electrical stimulus (ES) application. For this, the surface characteristics, mechanical, physicochemical, tribological, electrochemical, tribocorrosive, antimicrobial, biological, and osteogenic properties will be investigated. Commercially pure titanium discs will be allocated into 4 groups for the following surface treatments: (1) machined (2) PEO-treated; (3) treated with PEO followed by electrodeposition of PPy (PEO+PPy); (4) treated with PEO followed by electrodeposition of PPy and ZnO (PEO+PPy/ZnO). Surface characteristics and mechanical properties will be evaluated by physical and tribological tests, respectively. Electrochemical behavior will be assessed to evaluate the coatings' corrosion resistance. The antimicrobial effect of the coatings will be evaluated against monospecies and polymicrobial biofilms. The albumin and fetal bovine serum (FBS) protein interaction with the surfaces will be measured. The three groups described above (controls) and three more groups that will undergo ES (experimental) will be considered for the ES stage. In order to evaluate the effect of ES, pre-osteoblastic cells will be used. In addition, the influence of ES parameters on the Zn2+ ion release mechanism and on the modulation of the microbiota composition will also be investigated. The quantitative data will be analyzed statistically considering ± = 0.05. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SAHA, SOUMYA; COSTA, RAPHAEL CAVALCANTE; SILVA, MIRELA CAROLINE; FONSECA-SANTOS, JOAO MATHEUS; CHEN, LIN; PHAKATKAR, ABHIJIT H.; BHATIA, HARSHDEEP; FAVERANI, LEONARDO P.; BARAO, VALENTIM A. R.; SHOKUHFAR, TOLOU; et al. Collagen membrane functionalized with magnesium oxide via room-temperature atomic layer deposition promotes osteopromotive and antimicrobial properties. BIOACTIVE MATERIALS, v. 30, p. 16-pg., 2023-12-01. (22/16267-5, 20/05231-4, 20/10436-4)

ANDRADE, CATIA SUFIA; BORGES, MARIA HELENA R.; SILVA, JOAO PEDRO; MALHEIROS, SAMUEL; SACRAMENTO, CATHARINA; RUIZ, KARINA G. S.; DA CRUZ, NILSON C.; RANGEL, ELIDIANE C.; FORTULAN, CARLOS; FIGUEIREDO, LUCIENE; et al. Micro-arc driven porous ZrO₂ coating for tailoring surface properties of titanium for dental implants application. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 245, p. 20-pg., 2024-09-17. (22/07353-5, 22/15677-5, 22/16267-5)

BORGES, MARIA HELENA R.; KANNIYAPPAN, HEMALATHA; SUN, YANI; NAGAY, BRUNA EGUMI; DA CRUZ, NILSON C.; MATHEW, MATHEW T.; BARAO, VALENTIM A. R.. Polypyrrole zinc-doped film deposited on a plasma-treated surface improves the tribocorrosion behavior of titanium dental implant material. SURFACE & COATINGS TECHNOLOGY, v. 475, p. 17-pg., 2023-11-08. (22/07353-5, 22/16267-5)

COSTA, RAPHAEL C.; NAGAY, BRUNA E.; VILLA, JAVIER E. L.; SOTOMAYOR, MARIA D. P. T.; NERES, LARIEL CHAGAS DA SILVA; BENSO, BRUNA; AGUAYO, SEBASTIAN; SACRAMENTO, CATARINA M.; RUIZ, KARINA G. S.; SPADA, FERNANDA P.; et al. Pathogenesis-Guided Engineering: pH-Responsive Imprinted Polymer Co-Delivering Folate for Inflammation-Resolving as Immunotherapy in Implant-Related Infections. ADVANCED FUNCTIONAL MATERIALS, v. 34, n. 42, p. 16-pg., 2024-07-23. (20/05231-4, 18/20719-3, 20/10436-4, 22/16267-5, 21/09434-0)

DINI, CAROLINE; YAMASHITA, KAREN MIDORI; SACRAMENTO, CATHARINA MARQUES; BORGES, MARIA HELENA ROSSY; TAKEDA, THAIS TERUMI SADAMITSU; SILVA, JOAO PEDRO DOS SANTOS; NAGAY, BRUNA EGUMI; COSTA, RAPHAEL CAVALCANTE; DA CRUZ, NILSON CRISTINO; RANGEL, ELIDIANE CIPRIANO; et al. Tailoring magnesium-doped coatings for improving surface and biological properties of titanium-based dental implants. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 246, p. 12-pg., 2025-02-01. (20/05231-4, 22/16267-5, 23/02180-8, 20/05234-3)

BORGES, GUILHERME ALMEIDA; MESQUITA, MARCELO FERRAZ; MATIAS, LUANA FIGUEIREDO DA SILVA; BARBIN, THAIS; MACHADO, LEONARDO MENDES RIBEIRO; BARAO, VALENTIM ADELINO RICARDO. Carbon fiber-reinforced PEEK as a framework material for single implant-retained mandibular overdentures. BRAZILIAN ORAL RESEARCH, v. 39, p. 11-pg., 2025-01-01. (22/16267-5)

PEREIRA, MARTAMARIA ALVES; PIAZZA, RODOLFO; SANTANA, AMANDA PAINO; BARAO, VALENTIM ADELINO RICARDO; MALHEIROS, SAMUEL SANTANA; VAN DEN BEUCKEN, JEROEN J. J. P.; DE MOLON, RAFAEL SCAF; DE AVILA, ERICA DORIGATTI. Unraveling the Applicability of LbL Coatings for Drug Delivery in Dental Implant-Related Infection Treatment. ACS BIOMATERIALS SCIENCE & ENGINEERING, v. N/A, p. 20-pg., 2024-11-30. (18/20719-3, 21/10762-1, 22/16267-5, 23/15750-7, 21/09434-0)

DINI, CAROLINE; BORGES, MARIA HELENA ROSSY; MALHEIROS, SAMUEL SANTANA; PIAZZA, RODOLFO DEBONE; VAN DEN BEUCKEN, JEROEN J. J. P.; DE AVILA, ERICA DORIGATTI; SOUZA, JOAO GABRIEL S.; BARAO, VALENTIM A. R.. Progress in Designing Therapeutic Antimicrobial Hydrogels Targeting Implant-associated Infections: Paving the Way for a Sustainable Platform Applied to Biomedical Devices. ADVANCED HEALTHCARE MATERIALS, v. N/A, p. 35-pg., 2024-10-23. (22/15677-5, 18/20719-3, 22/07353-5, 22/16267-5, 20/05234-3, 21/09434-0, 23/02180-8)

BORGES, MARIA HELENA R.; NAGAY, BRUNA E.; SOUZA, JOAO GABRIEL S.; BARAO, VALENTIM A. R.. What challenges hinder the adoption of antimicrobial surface in the dental implant market?. Expert Review of Medical Devices, v. 21, n. 12, p. 5-pg., 2024-12-13. (22/16267-5, 22/07353-5, 24/15196-2, 24/02209-9)