Non-aureus staphylococci: potential beneficial implications for udder health

• Grant number: 22/11623-8
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2023
• End date: September 30, 2025
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Nathalia Cristina Cirone Silva
• Grantee: Nathalia Cristina Cirone Silva
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Bruno Toledo Silva ; Fernando Nogueira de Souza ; Jeroen De Buck ; Sarne de Vliegher
• Associated scholarship(s): 25/01909-0 - Quantitative inhibition study of S. aureus by non-aureus Staphylococcus, BP.TT

Abstract

Mastitis is the most prevalent disease in dairy herds that affects world dairy production. The main pathogens involved are: Staphylococcus aureus, Streptococcus sp., and Escherichia coli. In addition to these, many studies cite non-aureus staphylococci as agents of mastitis, but also as agents with a potential for preventing mastitis caused by other pathogens. Thus, the objective of this work is to sequence the entire genome and evaluate the competition potential of non-aureus staphylococci (NAS) isolated from cow's milk against mastitis-causing pathogens. Ninety-six NAS strains (S. chromogenes, S. haemolyticus, S. warneri, S. simulans, S. xylosus. S. epidermidis e S. cohnii) isolated from milk from healthy and subclinical mastitis cows will be used to evaluate their antimicrobial action against previously isolated mastitis pathogens: S. aureus, S. uberis, S. dysgalactiae, S. agalactiae, and E. coli. First, a screening will be carried out to verify if the NAS strains inhibit the growth of pathogens, then quantitative growth inhibition assay will be performed and later the whole genome sequence of NAS strains will be performed for identification of the genetic elements behind the inhibitory action. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CIEZA, MIRIAN YULIZA RUBIO; BONSAGLIA, ERIKA CAROLINA ROMAO; RALL, VERA LUCIA MORES; DOS SANTOS, MARCOS VEIGA; SILVA, NATHALIA CRISTINA CIRONE. Staphylococcal Enterotoxins: Description and Importance in Food. PATHOGENS, v. 13, n. 8, p. 21-pg., 2024-08-01. (22/13775-0, 22/11623-8)