Production of personalized biological dressings for veterinary medicine - BioHealTech

• Grant number: 23/10608-8
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE
• Start date: March 01, 2024
• End date: November 30, 2024
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Agreement: SEBRAE-SP
• Principal Investigator: Leandro Norberto da Silva Júnior
• Grantee: Leandro Norberto da Silva Júnior
• Company: Leandro Norberto da Silva Junior Pesquisa e Desenvolvimento em Biotecnologia Ltda
• CNAE: Atividades de apoio à pecuária
• City: Marília
• Associated researchers: João Victor Barbosa Tenório Fireman

Abstract

Injuries to the skin, subcutaneous tissue and adjacent musculature are among the most common morbidities in the veterinary routine, whether due to traumatic causes (bite, car trauma, laceration caused by a sharp object, thermal and chemical abrasions), iatrogenic (surgical wounds created for resection of lesions and neoplasms) or congenital anomalies. For the closure of large skin wounds, reconstructive surgery is commonly performed in veterinary medicine, using various techniques such as pedicled axial or subdermal pattern flaps, geometric pattern plastic surgery, relief incisions and skin grafts. The latter refer to free skin segments, which can be transferred to a location distant from the donor site (autograft), between different individuals of the same species (allograft) and even between different species (xenograft). The technology used by Tissue Engineering meets this challenge of Regenerative Therapy with efficiency in skin repairs, through the development of compatible biomaterials (decellularized and recellularized biological scaffolds). This new multidisciplinary specialty aims to produce artificial supports, biological or not, intended for cell growth, reproducing the characteristics of the extracellular matrix (ECM). In this project, derms from different species of decellularized production animals will be used to obtain acellular scaffolds, with previously established protocols in other animal models. Histological analysis, scanning electron microscopy and quantification of genomic DNA will be performed to prove the absence of cells in the samples after the process, validating the scaffolds for making a biomaterial, biogel, or biocompound for making products from association with drugs. In order to evaluate the active principles of the biomaterial from the different decellularized dermis, the biomaterial will be evaluated in vitro. Several studies indicate that biomaterials composed of extracellular matrix have a great influence on tissue reconstruction, since they provide not only signaling, but also a stock of components for the development of a new tissue. In this way, the company aims to develop this potential pharmacological product for veterinary medicine in the form of personalized bio-bandages for application in regenerative medicine for animals of high zootechnical value. The proposal is to create a laboratory that remotely serves veterinarians, clinics, hospitals and tutors in the treatment of skin lesions. In the future, we plan to build a network of laboratories arranged in a geographically strategic way to serve regions with a high density of producers and breeders of high zootechnical value animals, and may also serve large centers focused on the pet market. The validation stage of the biomaterial and its modulations will take place in such a way that it will be incubated by the Laboratory of Stem Cells and Tissue Engineering in the form of a partnership for the development of scientific research in the development of biomaterials. (AU)