Bacteriological and proteomic condition during endodontic treatment of teeth with necrotic pulps and periapical lesion in patients with type II diabetes mellitus: a randomized clinical trial

• Grant number: 23/10972-1
• Support Opportunities: Regular Research Grants
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Flaviana Bombarda de Andrade
• Grantee: Flaviana Bombarda de Andrade
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Associated researchers: Carlos Antonio Negrato ; Marco Antonio Hungaro Duarte ; Marília Afonso Rabelo Buzalaf

Abstract

Apical periodontitis occurs as a reaction to the presence of bacteria and their by-products in the root canal system (RCS), and it is characterized by the presence of inflammation and bone destruction in the periapical tissues. The possible connection between this periapical disease and systemic health is an issue that has been gaining importance in the medical and dental scientific community, and the correlation between systemic diseases, such as diabetes mellitus (DM), and the occurrence of apical periodontitis has been strongly suggested. This study aims to evaluate differences between patients with type II DM and non-diabetic patients through the microbiological and proteomic profile, and levels of lipopolysaccharides (LPS) in the root canals, as well as the regression of the volume of the periapical lesion after endodontic treatment. 34 patients will be selected, divided into two groups: 17 patients with type II DM and 17 non-diabetic patients, as a control. All subjects will receive conventional endodontic treatment with the same operator. Samples will be taken at the beginning of the treatment, after the coronal access (S1), after the chemical-mechanical preparation (S2) and after the removal of the intracanal medication (S3), already in the second session. The collections will be submitted to quantitative microbiological, proteomic and LPS analyses. Cone beam computerized tomography will be performed before and after six months of treatment finalization for diagnosis and monitoring of regression of periapical lesions, correlating their volume with other findings. The data obtained will be evaluated for normality and then submitted to the corresponding tests, aiming to accept or reject the null hypothesis that diabetic patients have the same endodontic conditions regarding the microbiological, proteomic and apical repair capacity as non-diabetic patients. (AU)