In vitro action of medicinal plant extracts on trap release extracellular neutrophils induced chemically and by sera from patients with psoriatic arthritis.

• Grant number: 24/01117-3
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2025
• End date: January 31, 2027
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Claudia Goldenstein Schainberg
• Grantee: Claudia Goldenstein Schainberg
• Host Institution: Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Ana Paula Pereira Velosa ; Candida Aparecida Leite Kassuya ; Marcia Midori Shinzato ; Solange Carrasco ; Walcy Paganelli Rosolia Teodoro
• Associated scholarship(s): 25/05004-1 - In vitro action of medicinal plant extracts on the release of extracellular traps of neutrophils induced chemically and by sera from patients with psoriatic arthrit, BP.TT

Abstract

In Brazil, several plants are used for the treatment of arthritis by the population. Studies have shown that these plants actually have anti-inflammatory action, especially in experimental models of acute arthritis. In addition to the anti-inflammatory effect, extracts from some plants have also a protective effect on the cardiovascular system, making them interesting for use in inflammatory rheumatological diseases that occur with cardiovascular comorbidities.In addition to their classic functions in defense against pathogenic microorganisms, neutrophils have the ability to modulate innate and adaptive immune responses. The release of neutrophil extracellular traps (NETS), despite being an important defense mechanism against infections, when aberrant and prolonged, is associated with the pathophysiology of several inflammatory autoimmune diseases and cardiovascular diseases. Patients with psoríasis have a higher amount of NETS in the peripheral blood than people without psoriasis and their sera have a greater ability to induce NETS. There is a correlation between these two parameters and the severity of the disease. Therefore, NETS are increasingly becoming a potential target for inflammatory diseases treatment. Extracts and compounds from plants with an anti-inflammatory effect could have fewer side effects than conventional treatments currently used to treat inflammatory diseases and be more cost-effective than routinely used drugs and biologics. Therefore, this study proposes to evaluate the effect of plant extracts and compounds on the release of induced NETS in three ways: with phorbol 12-myristate 13-acetate (PMA) which induces NETS dependent on nicotine adenine dinucleotide phosphate (NADPH) oxidase (NOX ), calcium ionophore that induces NOX-independent NET and serum from patients with psoriatic arthritis in neutrophils from healthy persons. (AU)