ASSOCIATION OF 16S MICROBIOME WITH NETOSIS IN CANINE BREAST CARCINOMA AND ITS IMPACT ON NETOSIS MODULATION

• Grant number: 24/16211-5
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2026
• End date: January 31, 2029
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Noeme Sousa Rocha
• Grantee: Noeme Sousa Rocha
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• City of the host institution: Botucatu
• Associated researchers: Luciane Alarcão Dias-Melicio ; Luis Mauricio Montoya Flórez
• Associated scholarship(s): 26/00276-6 - Association of the 16S Microbiome with NETosis in Canine Mammary Carcinoma and Its Impact on the Modulation of NETosis, BP.DR

Abstract

This study investigates the interaction between the 16S microbiome and breast carcinoma, as well as exploring the effects of microbiome modulation in xenotransplanted mice on the mechanism of NETosis. Using detailed methodology, fecal samples and oral swabs will be collected from female dogs with breast carcinoma for 16S microbiome analysis. MCF-7/S0.5 cells will be cultured to enable xenotransplantation into mice, where the intestinal microbiome will be modulated with Enterococcus faecium. The research aims to elucidate the underlying mechanisms of the immune response and its interaction with the microbiome. The hypothesis posits that the composition and diversity of the microbiome will be altered in female dogs with breast carcinoma, and that selective manipulation of the intestinal microbiota will affect the activation of NETosis mechanisms. Objectives include the characterization of the microbiome, quantification of NETosis, and evaluation of the impact of microbiome modulation on the immune response. These studies may provide valuable insights for the treatment of breast carcinoma in dogs and potential clinical applications in humans. (AU)