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Cloning and characterization of novel human genes related to spectrin, ankyrin and anion exchangers

Grant number: 00/12216-8
Support type:Research Projects - Thematic Grants
Duration: October 01, 2001 - March 31, 2006
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Sara Teresinha Olalla Saad
Grantee:Sara Teresinha Olalla Saad
Home Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Co-Principal Investigators:Jose Antonio Rocha Gontijo

Abstract

The cell membranes are involved in different functions and are, basically, composed by a lipid bilayer, peripheral and integral proteins. The peripheral proteins make up the cytoskeleton and the integral proteins are commonly glicoproteins involved in water and ion transport, adhesion, signalling, etc. The organization of the erythrocyte cytoskeleton and its importance in maintaining erythrocyte shape and deformability are well established. Unexpectedly, recent studies have revealed that cytoskeletal networks are present in various intracellular structures that are involved in regulation of major cellular functions as clustering and regulation of proteins associated with the plasma membrane, control of morphogenesis and cell proliferation, protein sorting and trafficking in the GoIgi apparatus, regulation of transcription factors, etc. Band 3, the red cell anion-exchanger, connects the cytoskeleton to the lipid bilayer and detinitively corroborates for the integrity of the red cell membrane. Besides that, band 3 controls the plasma and intracellular pH It is also present in kidney and heart. During the last ten years, we have been studying proteins of the erythrocyte membrane , as for example, spectrin, ankyrin and band 3. At the present moment, our interests lay in the detection and characterization of new proteins of this family of genes, as well as the detection of the interaction of these proteins with others. With the Project Cancer Genome, financially supported by FAPESP, we selected several EST homologous to the proteins mentioned. We intend to extend these EST, sequence the new genes and characterize the proteins codified. In addition to this, using two-hybrid system, we intend to investigate new proteins which interact with the proteins mentioned above. It is our aim to determine the pattern of expression of these new proteins in the different human tissues, which will be performed by multi-tissue Northern-blotting, and to determine the cellular localization of new proteins using immunofluorescence. Finally, we intend to evaluate, by means of microarrays and macroarrays, the involvement of these proteins in the regulation of the expression of other genes and their role in normal and tumour tissues. (AU)

Articles published in Agência FAPESP about the research grant
Brazilian researchers uncover the role of proteins in metastasis  

Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BENTO‚ L.M.A.; FAGIAN‚ M.M.; VERCESI‚ A.E.; GONTIJO‚ J.A.R. Effects of NH4Cl-induced systemic metabolic acidosis on kidney mitochondrial coupling and calcium transport in rats. Nephrology Dialysis Transplantation, v. 22, n. 10, p. 2817-2823, 2007.
DE MAGALHÃES SARTIM‚ R.; FANTINATO MENEGON‚ L.; ROBERTA DE ALMEIDA‚ A.; ANTONIO ROCHA GONTIJO‚ J.; ALINE BOER‚ P. Development of hypertension in a pyelonephritis-induced model: The effect of salt intake and inability of renal sodium handling. RENAL FAILURE, v. 28, n. 6, p. 501-507, 2006.
BENTO‚ L.M.A.; CARVALHEIRA‚ J.B.C.; MENEGON‚ L.F.; SAAD‚ M.J.A.; GONTIJO‚ J.A.R. Effects of NH4Cl intake on renal growth in rats: role of MAPK signalling pathway. Nephrology Dialysis Transplantation, v. 20, n. 12, p. 2654-2660, 2005.
BOER‚ P.A.; MORELLI‚ J.M.; FIGUEIREDO‚ J.F.; GONTIJO‚ J.A.R. Early altered renal sodium handling determined by lithium clearance in spontaneously hypertensive rats (SHR): role of renal nerves. Life Sciences, v. 76, n. 16, p. 1805-1815, 2005.
E.L. COSTA; A.R. ALMEIDA; F.M. NETTO; J.A.R. GONTIJO. Effect of intraperitoneally administered hydrolyzed whey protein on blood pressure and renal sodium handling in awake spontaneously hypertensive rats. Brazilian Journal of Medical and Biological Research, v. 38, n. 12, p. 1817-1824, Dez. 2005.
PAULA BELLINE; PATRÍCIA DA SILVA MELO; MARCELA HAUN; FERNANDA BOUCAULT PALHARES; PATRÍCIA ALINE BOER; JOSÉ ANTÔNIO ROCHA GONTIJO; JOSÉ FRANCISCO FIGUEIREDO. Effect of angiotensin II and losartan on the phagocytic activity of peritoneal macrophages from Balb/C mice. Memórias do Instituto Oswaldo Cruz, v. 99, n. 2, p. 167-172, Mar. 2004.

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