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Neuroendocrine regulation and effects of stress on female reproduction

Grant number: 04/09638-9
Support type:Research Projects - Thematic Grants
Duration: March 01, 2005 - December 31, 2009
Field of knowledge:Biological Sciences - Physiology - Physiology of Organs and Systems
Principal Investigator:Janete Aparecida Anselmo Franci
Grantee:Janete Aparecida Anselmo Franci
Home Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Co-Principal Investigators:Celso Rodrigues Franci
Associated grant(s):07/57795-4 - Gloria Ellyn Hoffman | University of Maryland - Estados Unidos, AV.EXT
07/57796-0 - John Albert Russell | University of Edinburgh - Inglaterra, AV.EXT
Associated scholarship(s):07/58635-0 - Is the action of progesterone in GnRH neurons mediated by the locus coeruleus? A study using microdialysis, BP.PD

Abstract

The knowledge of the control mechanisms of fertility has several sorts of implications: social (e.g., in the human species, the option of conception or anti-conception, populational control, the therapeutical development for several pathologies), economic (e.g., improvement of the fertility of domestic or captive wild species to provide food and clothing) and environmental (e.g., maintenance or reestablishment of ecosystems equilibrium, environment repopulation). On the other hand, fertility in humans and other species can be markedly influenced by stress. However, the neuroendocrine mechanisms that have been altered by the environmental stimulation are still not entirely understood. Natural reproduction depends on complex interactions of the nervous system and hormones produced in the brain, pituitary, gonads and other organs in order to prepare the gametes and induct sexual behavior to ensure fertilization. Due to the difficulties inherent to the complexity of the cyclic hormonal variations in females, there are much more studies regarding to physiological mechanisms in males than in females. In Brazil, there are very few researches working in this field, among them, the ones who got together in the thematic project which ends this year (2000-2004) and now, in this new project that includes two foreign researchers. The objective of this project is to study: a) neural circuits and central neuromediators (norepinephrine, angiotensin II, neuropeptide Y, nitric oxide, leptin, serotonin, oxytocin) involved in tonic and cyclic control of gonadotropins and prolactin secretion as well as its modulation by ovarian steroids; b) the interaction between the neuroendocrine control and ovarian innervation on the secretion of steroidal hormones and c) the effects of stressor stimuli in the neonatal period or in adulthood on the control of reproductive functions. It will be employed techniques like: vaginal smearing, to follow the estrus cycle; egg counting; ovariectomy associated or not with hormonal replacement to compare the action of ovarian steroids and correlate them to the estrus cycle; adrenalectomy. to evaluate the action of adrenal-originated progesterone; electrolytic or chemical lesions and chemical stimulation, to study the function of brain structures in the control of the hypothalamus pituitary-ovary axis; jugular vein cannulation for serial blood sampling; radioimmunoassay for hormonal and neuromediators measurement in plasma and tissues; cerebral structures microdialysis and microdissection to collect material for hormones and neuromediators measurement; HPLC/ED for measurement of cathecolamines in brain structures; NO synthase activity measurement, to evaluate nitric oxide formation; immunohistochemistry to evaluate neuronal activity, localization and co-localization of neuromediators and hormones receptors; stressful stimuli (neonatal manipulation, restraint, immobilization, cold, ether or predator exposure); morphometry (total number of neurons in brain structures or the number of neurons expressing selected neuromodulators or receptors); RT-PCR, western-blotting, immunobloting e in situ hybridization, to evaluate the neuronal expression of neuromediators, receptors and intracellular messengers; computerized behavioral registers to evaluate the effects of hormones and stressful stimuli on behavioral;.electrophvsiologic registers to evaluate the effect of ovarian steroids on neuronal electrical activity in brain structures. (AU)

Articles published in Pesquisa FAPESP Magazine about the research grant:
Bedroom findings 

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DEL BIANCO-BORGES, B.; FRANCI, C. R. Estrogen-dependent post-translational change in the nitric oxide system may mediate the leptin action on LH and prolactin secretion. Brain Research, v. 1604, p. 62-73, APR 16 2015. Web of Science Citations: 2.
LUCIO-OLIVEIRA, F.; TRASLAVINA, G. A. A.; BORGES, B. D. B.; FRANCI, C. R. MODULATION OF THE ACTIVITY OF VASOPRESSINERGIC NEURONS BY ESTROGEN IN RATS REFED WITH NORMAL OR SODIUM-FREE FOOD AFTER FASTING. Neuroscience, v. 284, p. 325-336, JAN 22 2015. Web of Science Citations: 3.
LIMA, FERNANDA BARBOSA; OTA, FABIO HONDA; CABRAL, FERNANDA JANLZUR; BORGES, BRUNO DEL BIANCO; FRANCI, CELSO RODRIGUES. Estrogen, but not progesterone, induces the activity of nitric oxide synthase within the medial preoptic area in female rats. Brain Research, v. 1578, p. 23-29, AUG 26 2014. Web of Science Citations: 4.
KALIL, B.; LEITE, C. M.; CARVALHO-LIMA, M.; ANSELMO-FRANCI, J. A. Role of sex steroids in progesterone and corticosterone response to acute restraint stress in rats: sex differences. STRESS-THE INTERNATIONAL JOURNAL ON THE BIOLOGY OF STRESS, v. 16, n. 4, p. 452-460, JUL 2013. Web of Science Citations: 27.
DONATO, JR., JOSE; SILVA, RENATA J.; SITA, LUCIANE V.; LEE, SYANN; LEE, CHARLOTTE; LACCHINI, SILVIA; BITTENCOURT, JACKSON C.; FRANCI, CELSO R.; CANTERAS, NEWTON S.; ELIAS, CAROL F. The Ventral Premammillary Nucleus Links Fasting-Induced Changes in Leptin Levels and Coordinated Luteinizing Hormone Secretion. JOURNAL OF NEUROSCIENCE, v. 29, n. 16, p. 5240-5250, APR 22 2009. Web of Science Citations: 72.
CAMOZZATO, TATIANE S. C.; WINKELMANN-DUARTE, ELISA C.; PADILHA, CAMILA B.; MIGUEL, SANDRO P. R.; BONZANINI, LAISA; ANSELMO-FRANCI, JANETE A.; FERNANDES, MARILDA C.; LUCION, ALDO B. Neonatal handling reduces the number of cells in the medial preoptic area of female rats. Brain Research, v. 1247, p. 92-99, Jan. 2009. Web of Science Citations: 14.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.