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New stationary phases with polymers sorbed and immobilized on pure and metallized silicas: preparation, properties and applications


This project involves the development of a basic understanding of the preparation and the utilization of new stationary phases for reversed-phase high performance liquid chromatography (RP-HPLC). These new stationary phases are prepared by sorbing, and then immobilizing, polymers on appropriate supports, such as ultra-pure silicas or metal oxide modified silicas. The project will emphasize the use of chemical variables, such as different reagents, different concentrations, different temperatures, and kinetic determinations to develop a better comprehension of the nature of the metal-oxide modified supports and of the processes which are involved in the sorption and immobilization of the polymeric phases within the pore systems of the different HPLC support particles. Physical, chemical and chromatographic methods will be used to characterize the resulting phases. In this way, the chromatographic properties will be optimized to give more stable and more efficient columns. The most promising of the new phases will also be evaluated using samples of environmental or industrial importance. It is expected that a fundamental understanding of the physical and chemical processes involved in the preparation of these new stationary phases for use in RP-HPLC will result: in products with applications in both analytical and preparative separations of complex mixtures of real samples. (AU)