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Repair of damaged DNA and biological consequences

Grant number: 98/11119-7
Support type:Research Projects - Thematic Grants
Duration: January 01, 1999 - February 29, 2004
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Carlos Frederico Martins Menck
Grantee:Carlos Frederico Martins Menck
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Co-Principal Investigators:Márcio de Castro Silva Filho ; Marie-Anne van Sluys
Associated scholarship(s):01/01728-0 - Complementation of the DNA repair deficiency in human cells by transducing the gene XPA cDNA with recombinant adenovirus, BP.PD
01/00963-6 - Complementation of the DNA repair deficiency in human cells by transducing the gene XPC cDNA with recombinant adenovirus, BP.MS
99/03419-3 - Complementation of the DNA repair deficiency in human cells by transferring the gene XPC with recombinant adenovirus, BP.IC

Abstract

The proposal aims to study different aspects of the Molecular Biology of the repair of injured DNA in various systems. In animal cells we are developing genetic vectors to intervene in the repair system and thus obtain information on how these act on some levels, such as, for example, apoptosis, mutagenesis and recombination. In plants we are trying to identify functions of two genes (araXPB and thi1) cloned by us in the repair of DNA, either through in vitro trials or in the construction of transgenetic plants modified by these genes. Finally, we are continuing the previous work in which we studied the repair mechanisms of bacterial DNA responsible for the protection of the genome against lesions induced by singlet oxygen. (AU)

Articles published in Revista Pesquisa FAPESP about the project
Subtle damage from the sun 
Subtle damage from the sun 

Scientific publications (8)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CHABREGAS, SABRINA M.; LUCHE, DOUGLAS D.; VAN SLUYS, MARIE-ANNE; MENCK, CARLOS F. M.; SILVA-FILHO, MARCIO C. Differential usage of two in-frame translational start codons regulates subcellular localization of Arabidopsis thaliana THI1. Journal of Cell Science, v. 116, n. 2, p. 285-291, Jan. 2003.
CAVALCANTE‚ A.K.D.; MARTINEZ‚ G.R.; DI MASCIO‚ P.; MENCK‚ C.F.M.; AGNEZ-LIMA‚ L.F. Cytotoxicity and mutagenesis induced by singlet oxygen in wild type and DNA repair deficient Escherichia coli strains. DNA Repair, v. 1, n. 12, p. 1051-1056, 2002.
JORGE‚ S.A.C.; MENCK‚ C.F.M.; SIES‚ H.; OSBORNE‚ M.R.; PHILLIPS‚ D.H.; SARASIN‚ A.; STARY‚ A. Mutagenic fingerprint of ozone in human cells. DNA Repair, v. 1, n. 5, p. 369-378, 2002.
MUOTRI‚ A.R.; MARCHETTO‚ M.C.N.; SUZUKI‚ M.F.; OKAZAKI‚ K.; LOTFI‚ C.F.P.; BRUMATTI‚ G.; AMARANTE-MENDES‚ G.P.; MENCK‚ C.F.M. Low amounts of the DNA repair XPA protein are sufficient to recover UV-resistance. Carcinogenesis, v. 23, n. 6, p. 1039-1046, 2002.
MUOTRI‚ A.R.; MARCHETTO‚ M.C.N.; ZERBINI‚ L.F.C.; LIBERMANN‚ T.A.; VENTURA‚ A.M.; SARASIN‚ A.; MENCK‚ C.F.M. Complementation of the DNA repair deficiency in human xeroderma pigmentosum group A and C cells by recombinant adenovirus-mediated gene transfer. Human Gene Therapy, v. 13, n. 15, p. 1833-1844, 2002.
AGNEZ-LIMA‚ LF; NAPOLITANO‚ RL; FUCHS‚ RPP; DI MASCIO‚ P.; MUOTRI‚ AR; MENCK‚ CFM. DNA repair and sequence context affect 1O2-induced mutagenesis in bacteria. Nucleic Acids Research, v. 29, n. 13, p. 2899-2903, 2001.
CHABREGAS, SABRINA M.; LUCHE, DOUGLAS D.; FARIAS, LEONARDO P.; RIBEIRO, ALBERTO F.; VAN SLUYS, MARIE-ANNE; MENCK, CARLOS F. M.; SILVA-FILHO, MARCIO C. Dual targeting properties of the N-terminal signal sequence of Arabidopsis thaliana THI1 protein to mitochondria and chloroplasts. Plant Molecular Biology, v. 46, n. 6, p. 639-650, 2001.
CHIGANÇAS‚ V.; MIYAJI‚ E.N.; MUOTRI‚ A.R.; JACYSYN‚ J.F.; AMARANTE-MENDES‚ G.P.; YASUI‚ A.; MENCK‚ C.F.M. Photorepair prevents ultraviolet-induced apoptosis in human cells expressing the marsupial photolyase gene. Cancer Research, v. 60, n. 9, p. 2458, 2000.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.