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Studies on emerging viruses including arbovirus, robovirus, respiratory viruses and congenital transmission, at the Centro de Pesquisa em Virologia da Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo

Grant number: 08/50617-6
Support type:Research Projects - Thematic Grants
Duration: September 01, 2008 - December 31, 2012
Field of knowledge:Biological Sciences - Microbiology
Cooperation agreement: CNPq - PPSUS
Principal Investigator:Luiz Tadeu Moraes Figueiredo
Grantee:Luiz Tadeu Moraes Figueiredo
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Co-Principal Investigators:Aparecida Yulie Yamamoto ; Benedito Antônio Lopes da Fonseca ; Eurico de Arruda Neto ; Victor Hugo Aquino Quintana
Associated scholarship(s):11/20447-4 - Infections by arbovirus Oropouche, Mayaro, Chikungunya and West Nile Virus in wild birds in the State of São Paulo, BP.PD
09/14552-0 - Prospective study of dengue virus infection in children in Ribeirão Preto, São Paulo, BP.DR

Abstract

This 4 year-long thematic project, includes 7 main subprojects-sections subdivided into 42 research subprojects. Five principal researchers and 58 colaborator researchers will participate in the project, which will be developed in the virus research unit of the School of Medicine of the University of São Paulo in Ribeirão Preto, Brazil. Arboviruses and roboviruses of great importance in public health such as dengue, yellow fever, hantaviruses, aernaviruses, and viruses that cause infection of the central nervous system such as Rocio virus and Saint Louis encephalitis virus will be studied as part of this research project. Other studies will include agents that cause large outbreaks in Brazil such as Oropouche virus. Besides, other viruses will be part of the study because they represent an emergent threat of outbreaks in the most populated areas of the country. These are yellow fever. Oropouche, Saint Louis encephalitis and Mayaro viruses. Foreign viruses, such as West Nile virus, that probably will be introduced into Brazil and Bocavirus, a respiratory transmitted virus recently described also will be studied as part of this project. For studying these viruses, serologic ELISAs using recombinant proteins of Oropouche virus, Hantavirus and Arenavirus as antigens will be standardized and recombinant peptides of the E protein of Saint Louis encephalitis, Rocio and West Nile viruses will be produced. We will also standardize an enzyme immunoassay using arbovirus (dengue, Mayaro, Oropouche virus) infected cells as antigen and an unprecedented neutralization test for Hantavirus read by real time RT-PCR. Furthermore, we will detect arbovirus, robovirus, human bocavirus and human herpesvirus type 6 genomes by real time RT-PCR, and will try to isolate a Hantavirus for the first time in Brazil, in our biosafety level 3 laboratories. Studies on physiopathology of diseases will include a search for dengue and Hantavirus infections in platelets, a study on cardiac function in Hantavirus Cardiopulmonary Syndrome patients, a search for apoptosis in Oropouche virus infections and a study of the central nervous infection by Rocio virus in a mouse experimental model. We will also look for target tissues of infection and replication of human Bocavirus. Epidemiologic studies will be carried out searching for rodent reservoirs of Hantavirus and looking for antibodies to arbovirus and robovirus in Cássia dos Coqueiros County, SP, in the southern state of Santa Catarina and in the Amazonas state, in the North of Brazil. Equines also will be studied by a serologic survey looking for Saint Louis encephalitis, Rocio and West Nile virus infection. Molecular epidemiology studies will include genotyping dengue virus type 3 isolated in different regions of Brazil and Paraguay, as well as detected in patients with diseases of different severities. This part will include the analysis of genomes and phylogenetic studies of Saint Louis encephalitis virus, as well as other arboviruses and roboviruses that eventually will be isolated in our BSL3 facility. The full-genome phylogenesis of Brazilian Bocavirus also will be studied. Finally, we will perform studies on vaccines and antiviral drugs, evaluating the immunogenetic ability of recombinant peptides of the E protein of Rocio and Saint Louis encephalitis viruses; isolating and testing the immunogenicity of a cold-adapted mutant of Oropouche virus and testing the potential antiviral action of chloroquine against this virus. (AU)

Articles published in Agência FAPESP Newsletter about the research grant
Scientists investigate the role of the human bocavirus in the tonsils of children  
A Brazilian group is working to develop a vaccine for hantavirus 

Scientific publications (12)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
KHAN, MOHD JASEEM; TRABUCO, AMANDA CRISTINA; ALFONSO, HELDA LIZ; FIGUEIREDO, MARIO LUIS; BATISTA, WEBER CHELI; BADRA, SORAYA JABUR; FIGUEIREDO, LUIZ TADEU; LAVRADOR, MARCO AURELIO; AQUINO, VICTOR HUGO. DNA Microarray Platform for Detection and Surveillance of Viruses Transmitted by Small Mammals and Arthropods. PLoS Neglected Tropical Diseases, v. 10, n. 9 SEP 2016. Web of Science Citations: 5.
SABINO-SANTOS, JR., GILBERTO; MAIA, FELIPE G. M.; JONSSON, COLLEEN B.; GOODIN, DOUGLAS G.; SALAZAR-BRAVO, JORGE; FIGUEIREDO, LUIZ TADEU M. Serologic Evidence of Mammarenaviruses among Wild Rodents in Brazil. JOURNAL OF WILDLIFE DISEASES, v. 52, n. 3, p. 766-769, JUL 2016. Web of Science Citations: 2.
WILLIAM MARCIEL DE SOUZA; ALEX MARTINS MACHADO; LUIZ TADEU MORAES FIGUEIREDO. Experimental infection of Rio Mamore hantavirus in Sigmodontinae rodents. Memórias do Instituto Oswaldo Cruz, v. 111, n. 6, p. 399-402, Jun. 2016. Web of Science Citations: 1.
DE PADUA, MICHELLY; DE SOUZA, WILLIAM MARCIEL; LAURETTI, FLAVIO; FIGUEIREDO, LUIZ TADEU MORAES. Development of a novel plaque reduction neutralisation test for hantavirus infection. Memórias do Instituto Oswaldo Cruz, v. 110, n. 5, p. 624-628, AUG 2015. Web of Science Citations: 2.
SABINO-SANTOS, JR., GILBERTO; MOTTA MAIA, FELIPE GONCALVES; VIEIRA, THALLYTA MARIA; MUYLAERT, RENATA DE LARA; LIMA, SABRINA MIRANDA; GONCALVES, CRISTIELI BARROS; BARROSO, PATRICIA DOERL; MELO, MARIA NORMA; JONSSON, COLLEEN B.; GOODIN, DOUGLAS; SALAZAR-BRAVO, JORGE; MORAES FIGUEIREDO, LUIZ TADEU. Evidence of Hantavirus Infection among Bats in Brazil. American Journal of Tropical Medicine and Hygiene, v. 93, n. 2, p. 404-406, AUG 2015. Web of Science Citations: 7.
MULLER, VANESSA DANIELLE; SOARES, RICARDO OLIVEIRA; DOS SANTOS-JUNIOR, NILTON NASCIMENTO; TRABUCO, AMANDA CRISTINA; CINTRA, ADELIA CRISTINA; FIGUEIREDO, LUIZ TADEU; CALIRI, ANTONIO; SAMPAIO, SUELY VILELA; AQUINO, VICTOR HUGO. Phospholipase A(2) Isolated from the Venom of Crotalus durissus terrificus Inactivates Dengue virus and Other Enveloped Viruses by Disrupting the Viral Envelope. PLoS One, v. 9, n. 11 NOV 10 2014. Web of Science Citations: 20.
MOTTA MAIA, FELIPE GONCALVES; CHAVEZ, JULIANA HELENA; DE SOUZA, WILLIAM MARCIEL; ROMEIRO, MARILIA FARIGNOLI; DE CASTRO-JORGE, LUIZA ANTUNES; LOPES DA FONSECA, BENEDITO ANTONIO; MORAES FIGUEIREDO, LUIZ TADEU. Infection with Saint Louis encephalitis virus in the city of Ribeirao Preto, Brazil: report of one case. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, v. 26, p. 96-97, SEP 2014. Web of Science Citations: 6.
SILVA, JAQUELINE RAYMONDI; ROMEIRO, MARILIA FARIGNOLI; DE SOUZA, WILLIAM MARCIEL; MUNHOZ, THIAGO DEMARCHI; BORGES, GUSTAVO PUIA; BRIOSCHI SOARES, OTAVIO AUGUSTO; COELHO DE CAMPOS, CARLOS HENRIQUE; MACHADO, ROSANGELA ZACARIAS; CRISTINY RODRIGUES SILVA, MARIA LUANA; MAIA FARIA, JOICE LARA; CHAVEZ, JULIANA HELENA; MORAES FIGUEIREDO, LUIZ TADEU. A Saint Louis encephalitis and Rocio virus serosurvey in Brazilian horses. Revista da Sociedade Brasileira de Medicina Tropical, v. 47, n. 4, p. 414-417, JUL-AUG 2014. Web of Science Citations: 11.
MACHADO, ALEX MARTINS; DE SOUZA, WILLIAM MARCIEL; DE PADUA, MICHELLY; DA SILVA RODRIGUES MACHADO, ALINE RAFAELA; MORAES FIGUEIREDO, LUIZ TADEU. Development of a One-Step SYBR Green I Real-Time RT-PCR Assay for the Detection and Quantitation of Araraquara and Rio Mamore Hantavirus. Viruses-Basel, v. 5, n. 9, p. 2272-2281, SEP 2013. Web of Science Citations: 12.
SANTOS, RODRIGO I.; ALMEIDA, MARIANA F. P.; PAULA, FLAVIA E.; RODRIGUES, ALCIR HUMBERTO; SARANZO, ARIANE MATTIOLI; PAULA, ANDRE E.; SILVA, MARIA LUCIA; ALVES CORREA, VANI MARIA; ACRANI, GUSTAVO OLSZANSKI; NEDER, LUCIANO; ARRUDA, EURICO. Experimental infection of suckling mice by subcutaneous inoculation with Oropouche virus. VIRUS RESEARCH, v. 170, n. 1-2, p. 25-33, DEC 2012. Web of Science Citations: 11.
BADRA, SORAYA JABUR; MOTA MAIA, FELIPE GONCALVES; FIGUEIREDO, GLAUCIANE GARCIA; DOS SANTOS JUNIOR, GILBERTO SABINO; CAMPOS, GELSE MAZZONI; MORAES FIGUEIREDO, LUIZ TADEU; COSTA PASSOS, AFONSO DINIS. A retrospective serologic survey of hantavirus infections in the county of Cassia dos Coqueiros, state of Sao Paulo, Brazil. Revista da Sociedade Brasileira de Medicina Tropical, v. 45, n. 4, p. 468-470, JUL-AUG 2012. Web of Science Citations: 13.
PROENCA-MODENA, JOSE LUIZ; GAGLIARDI, TALITA BIANCA; DE PAULA, FLAVIA ESCREMIM; IWAMOTO, MARISA AKIKO; CRIADO, MIRIA FERREIRA; CAMARA, ATAIDE A.; ACRANI, GUSTAVO OLSZANSKI; LEITE CINTRA, OTAVIO AUGUSTO; CERVI, MARIA CELIA; DE PAULA ARRUDA, LUISA KARLA; ARRUDA, EURICO. Detection of Human Bocavirus mRNA in Respiratory Secretions Correlates with High Viral Load and Concurrent Diarrhea. PLoS One, v. 6, n. 6 JUN 20 2011. Web of Science Citations: 27.

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