Molecular markers of the P. vivax associates the genotypes of the circumsporozoite protein

• Grant number: 06/00982-4
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2006
• End date: March 31, 2009
• Field of knowledge: Biological Sciences - Parasitology - Protozoology of Parasites
• Principal Investigator: Ricardo Luiz Dantas Machado
• Grantee: Ricardo Luiz Dantas Machado
• Host Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). São José do Rio Preto , SP, Brazil
• City of the host institution: São José do Rio Preto

Abstract

The malaria infections caused by P. vivax have reached great ratios, in certain regions of the world. However, impediments as culture unfeasibility of this parasite in vitro, presence of parasitemias decreases associates with its natural infections and the adaptation difficulty of these isolated ones in monkeys harm the works development associates to the malaria vivax. Some studies have been considered in intention to test this hypothesis, from attempts of origin phylogenetics reconstruction of the malaria parasites, also regarding the P. vivax origin. The similarity between P. simiovale and P. vivax-like has also provoked intense quarrels regarding the evolution origin of this variant, as well as the one that if it relates to its clinical epidemiology and the involved vectors in the transmission. Of this form, the main objective of this work is to study the association of P. vivax molecular markers with its variant types VK210, VK247 and P. vivax-like, contributing for one better knowledge of the phylogenetics relations between Plasmodium species that infect the human and other primates. Blood samples of 266 malaria patients, diagnosised for the blood thick film and the technique of Nested-PCR will be used as P. vivax, of four Brazilian Amazon regions. P. ovale, P. simium and P. simiovale isolated also will be analyzed. The variant types of this parasite will be identified by means of the PCR-RFLP technique. The neutral molecular markers, ACP, L35C, NUAC, NUN53, PMSTPK, 18S RNAr and CSP, will be amplified, cloned and sequenced through specific primers. The amino acid sequences will be lined up by means of software’s specific, in order to favor the elaboration of possible phylogenetics relations between the analyzed parasites. (AU)

Scientific publications

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STORTI-MELO, LUCIANE M.; DE SOUZA-NEIRAS, WANESSA C.; CASSIANO, GUSTAVO C.; JOAZEIRO, ANA C. P.; FONTES, COR J.; BONINI-DOMINGOS, CLAUDIA R.; D'ALMEIDA COUTO, ALVARO A. R.; POVOA, MARINETE M.; DE MATTOS, LUIZ C.; CAVASINI, CARLOS E.; et al. Plasmodium vivax circumsporozoite variants and Duffy blood group genotypes in the Brazilian Amazon region. Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 103, n. 7, p. 672-678, JUL 2009. (06/00982-4)