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Association between early childhood caries and clinical, biochemical, microbiological, environmental and behavioral factors in pre-school children aged 36 months


Early childhood caries (ECC) is defined as the presence of one or more decayed, missing or filled dental surfaces in preschool children younger than 06 years. Epidemiologic studies have shown that in Brazil early childhood caries is a public health problem, and presents a high prevalence, that ranges from 24,2 to 43.4%. The literature review evidences that in spite of existing an extensive number of studies that have considered the subject above, none of them analyzed all the factors related to ECC etiology simultaneously. Thus, the aim of the present study is to define the early childhood caries prevalence in brazilian preschool children of 36 months. The biochemical and microbiological biofilm composition will also be evaluated as well as ambiental and behavioral factors. One hundred fifty children of both sex, attemding to public nursery schools and preschools in the city of Itatiba-SP will take part in the study. The children will be submitted to a clinical examination for the decayed, missing or filled-teeth (including initial lesions) determination and to a biofilm collection. The children responsible will have to fill a questionnaire about ambiental and behavioral factors. In the biochemcal analyses of the biofilm, calcium, fluoride, phosphorus and the insoluble polysaccharide will be determined though the following methods: atomic absorption spectrophotometry, an ion-selective electrode and colorimetry. These variables will be expressed through the dry biofilm weight. Quantitative microbiological culture techniques will be use to determine the number of mutans streptococci. The results of prevalence will be express in percentage and average caries index. The data will be statistically analyzed trough multiple logistic regression with 95% confidence interval. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PARISOTTO, THAIS M.; STEINER-OLIVEIRA, CAROLINA; DE SOUZA-E-SILVA, CINTIA M.; PERES, REGINA C. R.; RODRIGUES, LIDIANY K. A.; NOBRE-DOS-SANTOS, MARINES. Assessment of cavitated and active non-cavitated caries lesions in 3-to 4-year-old preschool children: a field study. International Journal of Paediatric Dentistry, v. 22, n. 2, p. 92-99, MAR 2012. Web of Science Citations: 9.
PARISOTTO, THAIS MANZANO; STEINER-OLIVEIRA, CAROLINA; DUQUE, CRISTIANE; ROCHA PERES, REGINA CELIA; AZEVEDO RODRIGUES, LIDIANY KARLA; NOBRE-DOS-SANTOS, MARINES. Relationship among microbiological composition and presence of dental plaque, sugar exposure, social factors and different stages of early childhood caries. ARCHIVES OF ORAL BIOLOGY, v. 55, n. 5, p. 365-373, MAY 2010. Web of Science Citations: 47.

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