Characterization of carlavirus infecting garlic (Allium sativum L.) in South and Southeast of Brazil

• Grant number: 07/03485-4
• Support Opportunities: Regular Research Grants
• Duration: September 01, 2007 - August 31, 2009
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Marcelo Agenor Pavan
• Grantee: Marcelo Agenor Pavan
• Host Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Garlic is propagated by bulbs thats why garlic are frequently infected by viruses. Garlic can be infected by virus begonging to the genus: Allexivirus, Carlavirus and Potyvirus. Garlic common latent virus (GCLV) and Shallot latent virus (SLV) are the most important carlavirus species infecting garlic around the world, and they are also aphid transmitted. In Brazil there is no information about the species of carlavirus occurring in garlic, so the objective of this work is to verify the occurrence and to identify the species of carlavirus infecting garlic in South and Southeast of Brazil, as well as developing molecular tools for the diagnosis of these species. Nucleotide alignment sequences between carlavirus species were used to design primers. This primers will be tested by RT-PCR on garlic samples containing virus particles typicalls of carlavirus. The fragments will be sequenced and specifics primers will be design for the species of carlavirus occurring on the garlic champ. (AU)