Bioguided prospection of chemoprevention from Eugenia jambolana, Garcinia xanthochymus e Alchomea glandulosa in hepatoma cell lines (Hepa 1c1c7 and HepG2)

• Grant number: 09/52716-4
• Support Opportunities: Regular Research Grants
• Duration: November 01, 2009 - March 31, 2012
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Christiane Pienna Soares
• Grantee: Christiane Pienna Soares
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The Brazilian geographic variability favors the development of different vegetal species, which stimulates the study in search of new bioactive agents. Among the species chosen for investigation of its possible chemopreventive effects are those that belong to Eugenia and Alchomea genus, that are widely known to contain phenolic substances with potential antioxidant, anti-inflammatory, analgesic, antipyretic, antifungal and cytotoxic effects in development of some cancer cell lines. Another specie chosen (Garcinia) for the present study belong to the Clusiaceae, and its chemical profile reveals the presence of xanthones, flavonoids, phenols and acridones as major groups of special metabolites, showing analgesic, antibacterial, antioxidant, antiviral and antitumor activity. The present study has the objective to make a bioguided prospection for chemoprevention activity in extracts, semi-purified fractions and mainly, in pure substances isolated from Eugenia jambolana, Garcinia xanthochymus and Alchomea glandulosa as part of the bioprospection of natural products. So far there are no studies about the chemoprevention capacity of these vegetal species. In the present study it'll be assessed the quinone-reductase enzyme induction in murine hepatoma cell line (Hepa 1c1c7); and the genotoxicity/antigenotoxicity in human hepatoma cells (HepG2), competent to xenobiotic metabolization, through the Comet assay. (AU)