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Evaluation of pregnant women genotoxicity with positive diagnosis of mild gestational hyperglycaemia

Abstract

The group IB corresponds to pregnant women bearers of mild hiperglycaemia, in other words, pregnant women who present positive screening, but negative diagnosis for diabetes normal gestacional(OGTT100g), and answer altered in the glycaemic profile (GP). This group was, accidentally, identified in 1983, when the project prospective was developed for standardization of PG comparing it with OGTT 100g in the diagnosis of the diabetes in the gestation (GDM). The hiperglicemia, main clinical manifestation of the Diabetes mellitus, generates species reactivate of oxygen (ROS) that alter proteins, lipids and DNA. As diabetic pregnant women present exacerbated oxidative stress, the present work has as objective to investigate the levels of oxidative damages caused by ROS in maternal and fetal DNA (8-OHdG). Besides, it will be evaluated the levels of 8-OHdG presented in the urine of those patients. They will be appraised women assisted at the Hospital of the Clinics of University of Medicine - UNESP, Botucatu - SP in the period of 1 year. 1) women with normal OGTT and GP (control group), 2) women with normal OGTT and abnormal PG (mild hiperglycaemia group), 3) women with abnormal OGTT and normal PG (gestational diabetes group), 4) women with abnormal OGTT and PG (overt gestational diabetes group). The newborn will be selected with base in the age gestacional (37 to 42 weeks). Samples of each adult individual's outlying blood, umbilical cord blood and urine will be collected, by experienced professional. The samples of blood collected will be processed immediately for determination of oxidative DNA damage(8-OHdG) while urine samples will be stored for future dosages of 8-OHdG by ELISA.The significance level for all test will be considered p<0,05. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GELALETI, RAFAEL BOTTARO; DAMASCENO, DEBORA C.; SANTOS, DANIELE P.; CALDERON, IRACEMA M. P.; RUDGE, MARILZA V. C. Increased DNA Damage is Related to Maternal Blood Glucose Levels in the Offspring of Women With Diabetes and Mild Gestational Hyperglycemia. REPRODUCTIVE SCIENCES, v. 23, n. 3, p. 318-323, MAR 2016. Web of Science Citations: 4.
GELALETI, RAFAEL B.; DAMASCENO, DEBORA C.; SALVADORI, DAISY M. F.; MARCONDES, JOAO PAULO C.; LIMA, PAULA H. O.; MORCELI, GLILCIANE; CALDERON, IRACEMA M. P.; RUDGE, MARILZA V. C. IRS-1 gene polymorphism and DNA damage in pregnant women with diabetes or mild gestational hyperglycemia. DIABETOLOGY & METABOLIC SYNDROME, v. 7, APR 2 2015. Web of Science Citations: 1.
GELALETI, RAFAEL BOTTARO; DAMASCENO, DEBORA CRISTINA; ORTIZ LIMA, PAULA HELENA; FAVERO SALVADORI, DAISY MARIA; PARANHOS CALDERON, IRACEMA DE MATTOS; PERACOLI, JOSE CARLOS; CUNHA RUDGE, MARILZA VIEIRA. Oxidative DNA damage in diabetic and mild gestational hyperglycemic pregnant women. DIABETOLOGY & METABOLIC SYNDROME, v. 7, JAN 15 2015. Web of Science Citations: 9.

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