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Functional activity of polymorphonuclear cells from blood of newborn kids, in vitro for E. coli and S. aureus: influence of age and colostrum management


The aim of this study is to evaluate the in vitro functional activity of polymorphonuclear cells from periphertal blood of newborn kids by flow citometry- oxidative metabolism and phagocytosis induced by Staphylococcus aureus and Escherichia coli. Third two Saanen kids will be used. The healthy status will also assessed during the first fifteen days of life. These animals were shared in four groups with 8 animals each, as described: G1 - will received goat colostrum in nature; G2- goat colostrum heated at 56ºC for 60 minutes; G3 - cow colostrum and G4 - cow milk. Therefore, the animals of all groupus will received only cow milk equivalent of 10% of their body weight divided in two feeding. Pheripheral blood samples will be take to evaluate the influence of the manegement, age , and the polymorphonuclears responde induced by S. aureus and E. coli in the followinf periods: time 0 (before the colostrum intake), time 1 (48h after birth), time 2 (72-96h after birth), time 3 (168-192h after birth) e time 4 (336-360h after birth). The statistical analysis will be performed by variance analysis to do comparison among groups and inner group at the significance level of 5%. (AU)

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