Denture stomatitis in the senescent population: evaluation of activation and production of pro- and anti-inflammatory cytokines by monocytes

• Grant number: 08/03539-0
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2008
• End date: April 30, 2011
• Field of knowledge: Health Sciences - Dentistry
• Agreement: CNPq - PPSUS
• Principal Investigator: Vanessa Soares Lara
• Grantee: Vanessa Soares Lara
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

Denture stomatitis, the most frequent lesion in elderly removable prothesis wearers, is characterized by an inflammation of the denture-bearing mucosa, especially underlying maxillary prostheses. It is related with the presence of Candida albicans (C. albicans) and local and systemic factors, such as the impaired immune response, which is common in elderly people. Monocytes play an important role in the development of a protective immune response against fungal infections since they release cytokines which recruit and activate leukocytes, collaborating to the fungal clearance. There are no literature data regarding the immunomodulatory function of monocytes by elderly people presenting denture stomatitis. Therefore, the aim of the present study is to evaluate the activation and production of pro- and anti-inflammatory cytokines by monocytes isolated from peripheral blood, challenged in vitro with C. albicans, in elderly people, wearing complete maxillary denture, with or not denture stomatitis, in comparison to elderly people non-denture wearers. Peripheral blood will be obtained from volunteer donors and the monocytes will be purified and cultured in culture plates presenting glass coverslip inside the wells, with and without lipopolysaccharide (LPS) or viable C. albicans ATCC 10231. After incubation for 18 hours, supernatants will be harvested for measurement of the pro-inflammatory cytokines interleukin-1 alpha (IL-1±), interleukin-1 beta (IL-1²), IL-6, IL-8, monocyte chemoattractant protein (MCP-1) and tumor necrosis factor-alpha (TNF-±), and the anti-inflammatory cytokines IL-10 and transforming growth factor-beta (TGF-²), by enzyme immunoassay (ELISA).The expression of the monocyte marker, CD-14, and MCP-1 receptor and granulocyte-monocyte colony-stimulating factor (GM-CSF) receptor will be evaluated in the coverslip presenting adherent monocytes, by means of immunofluorescence. The results will be expressed as mean ± standard deviation (SD) of the values obtained by each group and the statistical analysis will be performed using analysis of variance (ANOVA), followed by the Tukey test. P values less than 0.05 will be considered statistically significant. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TOBOUTI, PRISCILA LIE; CASAROTO, ANA REGINA; COUTO DE ALMEIDA, RICARDO SERGIO; RAMOS, SOLANGE DE PAULA; DIONISIO, THIAGO JOSE; PORTO, VINICIUS CARVALHO; SANTOS, CARLOS FERREIRA; LARA, VANESSA SOARES. Expression of Secreted Aspartyl Proteinases in an Experimental Model of Candida albicans-Associated Denture Stomatitis. JOURNAL OF PROSTHODONTICS-IMPLANT ESTHETIC AND RECONSTRUCTIVE DENTISTRY, v. 25, n. 2, p. 127-134, FEB 2016. (08/03539-0)

PINKE, KAREN HENRIETTE; FREITAS, PATRICIA; VIERA, NARCISO ALMEIDA; HONORIO, HEITOR MARQUES; PORTO, VINICIUS CARVALHO; LARA, VANESSA SOARES. Decreased production of proinflammatory cytokines by monocytes from individuals presenting Candida-associated denture stomatitis. CYTOKINE, v. 77, p. 145-151, JAN 2016. (08/03539-0)