Evaluation of yerba maté (Ilex paruaguariensis) phenolic acids bioavailability

• Grant number: 09/14853-0
• Support Opportunities: Regular Research Grants
• Duration: May 01, 2010 - April 30, 2012
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Deborah Helena Markowicz Bastos
• Grantee: Deborah Helena Markowicz Bastos
• Host Institution: Faculdade de Saúde Pública (FSP). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Yerba Maté is a plant rich in polyphenols. In order to determine mechanisms by which yerba mate beverages exert biological activities correlated with health maintenance (antioxidant, antinflamatory and antiglycation, among others), studies of these bioactive compounds bioavailability and derivatives metabolites are necessary. The aim of this project is to evaluate yerba mate phenolic acids bioavailability in vivo (wistar rats) and in vitro. Animal groups will receive either yerba mate, water or phenolic acid standard. The identification and quantification of phenolic acids and metabolites in the in vivo and in vitro studies will be carried out by HPLC-diode array and HPLC-ESI-MS. For the in vivo study, the animals will be divided into 3 groups: Yerba Mate (extract of yerba mate), Standard (solution of 5-CQA) and control (saline). The doses will be defined after a pilot study. 7 days after receiving the corresponding drink through orogastric cannula (gavage), animals will be sacrificed at times 30, 60, 120, 240 and 480 minutes after the last gavage (n = 5 animals per group at each time) and their tissues will be submitted to chromatographic analysis. The following in vitro study will be done: identification and quantification of phenolic acids and metabolites formed after incubation of solutions of yerba mate (Test), 5-CQA (Standard) and saline (Control) with the cytosolic and microsomal fractions of liver and intestinal cells of Wistar rats. The data will be analyzed by ANOVA with one or two factors and Tukey post hoc test (for one-factor ANOVA). The significance level ± is 5%. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, DANIELA M.; PINTO, CAROLINA B.; SAMPAIO, GENI R.; YONEKURA, LINA; CATHARINO, RODRIGO R.; BASTOS, DEBORAH H. M.. Development and Validation of Methods for the Extraction of Phenolic Acids from Plasma, Urine, and Liver and Analysis by UPLC-MS. Journal of Agricultural and Food Chemistry, v. 61, n. 25, p. 6113-6121, JUN 26 2013. (08/10308-4, 09/01275-8, 09/14853-0)