Clinical, microbiological and imunological analyses in individuals with genetic susceptibility to periodontal disease

• Grant number: 09/08773-3
• Support Opportunities: Regular Research Grants
• Duration: September 01, 2009 - February 29, 2012
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Raquel Mantuaneli Scarel Caminaga
• Grantee: Raquel Mantuaneli Scarel Caminaga
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Periodontal disease is a multifactorial disease which is influenced by factors such as the presence of periodontopathogenic microorganisms, the reaction of the immune system, smoking, genetic susceptibility, stress and the occurrence of systemic diseases. The inflamed periodontal tissue produces several cytokines like Interleukin 4 (IL-4) and Interleukin 8 (IL-8). Recent studies carried out by this group investigated IL8 and IL4 gene polymorphisms in patients with and without periodontitis. In those studies were observed individuals with a genetic predisposition to periodontitis even after adjusting for covariates such as age, gender, skin color and smoking (results under submission to publication). The purpose of this study is to test the hypothesis that the major genetic susceptibility to periodontitis due to the carriage of some IL8 and IL4 haplotypes would be linked to differences in the cytokine levels and increased rates of periodontopathogens. One hundred and sixty individuals will be selected and grouped according to their genetic susceptibility into "Susceptible" and "Non-susceptible" groups, while into each group there will be patients With and Without Periodontitis. Also, we intend to verify whether individuals carrying a specific haplotype will show a better response to periodontal treatment and whether this would be influenced by the production of cytokines and/or by the presence of periodontopathogens. The selected individuals will be submitted to new periodontal exams, and the gingival fluid will be collected before the periodontal treatment and again 45 days after it. The IL-8 and IL-4 cytokines will be quantified through immunoenzymatic assays. It will be analyzed by absolute quantification the levels and proportions of Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola by Real Time PCR. This study is expected to contribute with a better understanding of the potential inter-relationship of different factors such as genetic, immunological and microbiological with periodontitis, since this disease is multifactorial. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FINOTI, LIVIA S.; CORBI, SAMIA C. T.; ANOVAZZI, GIOVANA; TEIXEIRA, SILVIA R. L.; CAPELA, MARISA V.; TANAKA, MARCIA H.; KIM, YEON J.; ORRICO, SILVANA R. P.; CIRELLI, JONI A.; MAYER, MARCIA P. A.; et al. Pathogen levels and clinical response to periodontal treatment in patients with Interleukin 8 haplotypes. PATHOGENS AND DISEASE, v. 69, n. 1, p. 21-28, OCT 2013. (09/11371-4, 09/08773-3, 03/10424-0)

FINOTI, L. S.; CORBI, S. C. T.; ANOVAZZI, G.; TEIXEIRA, S. R. L.; STEFFENS, J. P.; SECOLIN, R.; KIM, Y. J.; ORRICO, S. R. P.; CIRELLI, J. A.; MAYER, M. P. A.; et al. Association between IL8 haplotypes and pathogen levels in chronic periodontitis. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, v. 32, n. 10, p. 1333-1340, OCT 2013. (09/11371-4, 09/08773-3, 03/10424-0)

ANOVAZZI, GIOVANA; FINOTI, LIVIA S.; CORBI, SAMIA C. T.; KIM, YEON J.; MARCACCINI, ANDREA M.; GERLACH, RAQUEL F.; CAPELA, MARISA V.; ORRICO, SILVANA R. P.; CIRELLI, JONI A.; SCAREL-CAMINAGA, RAQUEL M.. Interleukin 4 haplotypes of susceptibility to chronic periodontitis are associated with IL-4 protein levels but not with clinical outcomes of periodontal therapy. HUMAN IMMUNOLOGY, v. 74, n. 12, p. 1688-1695, DEC 2013. (09/08773-3, 09/11321-7, 03/10424-0)

FINOTI, L. S.; ANOVAZZI, G.; PIGOSSI, S. C.; CORBI, S. C. T.; TEIXEIRA, S. R. L.; BRAIDO, G. V. V.; KIM, Y. J.; ORRICO, S. R. P.; CIRELLI, J. A.; MAYER, M. P. A.; et al. Periodontopathogens levels and clinical response to periodontal therapy in individuals with the interleukin-4 haplotype associated with susceptibility to chronic periodontitis. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, v. 32, n. 12, p. 1501-1509, DEC 2013. (09/11371-4, 09/08773-3, 03/10424-0)

CORBI, SAMIA C. T.; ANOVAZZI, GIOVANA; FINOTI, LIVIA S.; KIM, YEON J.; CAPELA, MARISA V.; SECOLIN, RODRIGO; MARCACCINI, ANDREA M.; GERLACH, RAQUEL F.; ORRICO, SILVANA R. P.; CIRELLI, JONI A.; et al. Haplotypes of susceptibility to chronic periodontitis in the Interleukin 8 gene do not influence protein level in the gingival crevicular fluid. ARCHIVES OF ORAL BIOLOGY, v. 57, n. 10, p. 1355-1361, OCT 2012. (09/08773-3, 03/10424-0)

CORBI, S. C. T.; FINOTI, L. S.; ANOVAZZI, G.; TANAKA, M. H.; KIM, Y. J.; SECOLIN, R.; MARCACCINI, A. M.; GERLACH, R. F.; ORRICO, S. R. P.; CIRELLI, J. A.; et al. Clinical outcomes of periodontal therapy are not influenced by the ATC/TTC haplotype in the IL8 gene. JOURNAL OF PERIODONTAL RESEARCH, v. 49, n. 4, p. 489-498, AUG 2014. (09/08773-3, 03/10424-0)

CIRELLI, THAMIRIS; FINOTI, LIVIA S.; CORBI, SAMIA C. T.; ANOVAZZI, GIOVANA; NEPOMUCENO, RAFAEL; ORRICO, SILVANA R. P.; CIRELLI, JONI A.; MAYER, MARCIA P. A.; SCAREL-CAMINAGA, RAQUEL M.. Absolute quantification of Aggregatibacter actinomycetemcomitans in patients carrying haplotypes associated with susceptibility to chronic periodontitis: multifaceted evaluation with periodontitis covariants. PATHOGENS AND DISEASE, v. 75, n. 7, OCT 2017. (09/11371-4, 13/06365-0, 09/11321-7, 09/08773-3)