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Effects of photodynamic therapy and essential oils of the Cymbopogon citratus (DC.) Stapf, Casearia sylvestris SW and Curcuma zedoaria Roscoe, in vitro, in biofilms of Candida albicans, Staphylococcus aureus and Streptococcus mutans

Abstract

The aim of this study is to evaluate the effects of photodynamic therapy (TFP) and antimicrobial activity of essential oils from Cymbopogon citratus (DC.) Stapf, Curcuma zedoaria Roscoe and Casearia sylvestris SW in the development of biofilm, produced by C. albicans, S. aureus and S. mutans and associations. In the assessment of essential oils effects and photodynamic therapy, a biofilm will be produced with standard microorganisms of C. albicans (ATCC 18804), S. aureus (ATCC 6538) and S. mutans (ATCC 35688) in acrylic disks for 5 days in sterile brain heart infusion both (BHI) containing 5 % sucrose. After this time it will be performed the photodynamic therapy with AsGaAl laser and photosensitivity methylene blue and the essential oils will be tested by a period of 5 minutes. The biofilm will be dispersed by ultrasound and serial dilutions will be carried out and aliquots plated in selective agar to the numbers of colony-forming units by milliliters (CFU/mL). Also, the phytoterapy and TFP effects will be evaluated in microrganisms of planktonic form. For the minimal inhibition concentration (MIC) of the essential oils it will be utilized 10 C. albicans, 10 S. aureus and 10 5. mutans clinical samples. The minimal inhibition concentration (MIC) will be made by serial dilutions. The result will be converted in logarithms of base 10 (logio) and analyzed statistically by the t Student (p³0,05) test. To illustrate the adherence of microorganisms C. albicans, S. aureus and S. mutans and associations a Scanning Electron Microscopy images (SEM) will be performed. (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FREIRE, FERNANDA; BORGES PEREIRA COSTA, ANNA CAROLINA; PEREIRA, CRISTIANE APARECIDA; BELTRAME JUNIOR, MILTON; JUNQUEIRA, JULIANA CAMPOS; CARDOSO JORGE, ANTONIO OLAVO. Comparison of the effect of rose bengal- and eosin Y-mediated photodynamic inactivation on planktonic cells and biofilms of Candida albicans. Lasers in Medical Science, v. 29, n. 3, p. 949-955, MAY 2014. Web of Science Citations: 24.
COELHO OLIVEIRA, SARAH ALMEIDA; MINA ZAMBRANA, JESSICA RABELO; REIS DI IORIO, FERNANDA BISPO; PEREIRA, CRISTIANE APARECIDA; CARDOSO JORGE, ANTONIO OLAVO. The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms. Brazilian Oral Research, v. 28, n. 1, p. 22-27, JAN-MAR 2014. Web of Science Citations: 7.
PEREIRA, CRISTIANE APARECIDA; BORGES PEREIRA COSTA, ANNA CAROLINA; CARREIRA, CLAUDIA MOURA; JUNQUEIRA, JULIANA CAMPOS; CARDOSO JORGE, ANTONIO OLAVO. Photodynamic inactivation of Streptococcus mutans and Streptococcus sanguinis biofilms in vitro. Lasers in Medical Science, v. 28, n. 3, p. 859-864, MAY 2013. Web of Science Citations: 32.
BORGES PEREIRA COSTA, ANNA CAROLINA; CAMPOS RASTEIRO, VANESSA MARIA; HALTER DA SILVA HASHIMOTO, EMILY SETSUKO; ARAUJO, CASSIA FERNANDES; PEREIRA, CRISTIANE APARECIDA; JUNQUEIRA, JULIANA CAMPOS; CARDOSO JORGE, ANTONIO OLAVO. Effect of erythrosine- and LED-mediated photodynamic therapy on buccal candidiasis infection of immunosuppressed mice and Candida albicans adherence to buccal epithelial cells. ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY, v. 114, n. 1, p. 67-74, JUL 2012. Web of Science Citations: 31.
BORGES PEREIRA COSTA, ANNA CAROLINA; DE CAMPOS RASTEIRO, VANESSA MARIA; PEREIRA, CRISTIANE APARECIDA; HALTER DA SILVA HASHIMOTO, EMILY SETSUKO; BELTRAME JUNIOR, MILTON; JUNQUEIRA, JULIANA CAMPOS; CARDOSO JORGE, ANTONIO OLAUO. Susceptibility of Candida albicans and Candida dubliniensis to erythrosine- and LED-mediated photodynamic therapy. ARCHIVES OF ORAL BIOLOGY, v. 56, n. 11, p. 1299-1305, NOV 2011. Web of Science Citations: 39.
PEREIRA, CRISTIANE APARECIDA; ROMEIRO, ROGERIO LIMA; BORGES PEREIRA COSTA, ANNA CAROLINA; SILVA MACHADO, ANA KARINA; JUNQUEIRA, JULIANA CAMPOS; CARDOSO JORGE, ANTONIO OLAVO. Susceptibility of Candida albicans, Staphylococcus aureus, and Streptococcus mutans biofilms to photodynamic inactivation: an in vitro study. Lasers in Medical Science, v. 26, n. 3, p. 341-348, MAY 2011. Web of Science Citations: 103.

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