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Development of cationic liposomes shielded by hyaluronic acid for DNA vaccination against leishmaniasis

Grant number: 10/02203-8
Support type:Regular Research Grants
Duration: July 01, 2010 - June 30, 2012
Field of knowledge:Engineering - Chemical Engineering
Principal researcher:Lucimara Gaziola de la Torre
Grantee:Lucimara Gaziola de la Torre
Home Institution: Faculdade de Engenharia Química (FEQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Assoc. researchers: Gabriela de Sá Cavalcanti Corrêa

Abstract

This project focuses the technological development of cationic liposomes carrying LACK DNA and shielded by hyaluronic acid as intranasal gene vaccine against Leishmaniasis. The in vivo and intranasal LACK DNA efficiency was demonstrated by the Instituto de Biofísica Carlos Chagas Filho-RJ. The hyaluronic acid is a natural polisacaride with mucoadesive properties. It had been used in intranasal vaccines and it also increases the DNA delivery into cells when incorporated in cationic liposomes. The liposomes will be composed by egg phosphatidylcholine, L-alpha-dioleoyl phosphatidylethanolamine and 1,2-dioleoyloxy-3-trimethylammonium propane. The hyaluronic acid that will be used for liposomes shielding will have low molecular weight. The physico-chemical characterization will be zeta potential, morphology, mean diameter, polydispersity and size distribution. The LACK DNA incorporation will be evaluated using fluorescence probe accessibility, molar charge ratio for complete DNA incorporation into the liposome, plasmid integrity after processing, using agarose gel electrophoresis technique. The best formulation will be produced and send for in vivo evaluation at the Instituto de Biofísica Carlos Chagas - RJ. Our previous experience with cationic liposomes focused the development of a DNA vaccine against tuberculosis (one patent, one addition certificate and 4 publications) and scale up studies for liposome production and its complexation with DNA (FAPESP PIPE II). In this context, this project will contribute to the development of new nanostructures useful for intranasal gene vaccination against Leishmaniasis. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GASPERINI, ANTONIO A. M.; PUENTES-MARTINEZ, XIMENA E.; BALBINO, TIAGO ALBERTINI; RIGOLETTO, THAIS DE PAULA; CAVALCANTI CORREA, GABRIELA DE SA; CASSAGO, ALEXANDRE; PORTUGAL, RODRIGO VILLARES; DE LA TORRE, LUCIMARA GAZIOLA; CAVALCANTI, LEIDE P. Association between Cationic Liposomes and Low Molecular Weight Hyaluronic Acid. Langmuir, v. 31, n. 11, p. 3308-3317, MAR 24 2015. Web of Science Citations: 12.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.