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Assessment of genetic diversity of populations of Odontophrynus moratoi (Anura: Cycloramphidae) threatened species by microsatellite markers: basis for conservation actions

Grant number: 10/08291-6
Support type:Regular Research Grants
Duration: August 01, 2010 - July 31, 2013
Field of knowledge:Biological Sciences - Genetics - Animal Genetics
Principal Investigator:Shirlei Maria Recco-Pimentel
Grantee:Shirlei Maria Recco-Pimentel
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil


The toad endemic Odontophrynus moratoi of Botucatu region is now critically endangered due to restricted geographical distribution and evidence of declining populations. The preservation programs operate in the recovery of endangered populations and, in general, are based on maintaining the maximum amount of genetic diversity. So the first stages of these programs is to assess the genetic variability and distribution of this among populations. This genetic diversity have been assessed by molecular methods, and particularly the microsatellite markers has successfully accessed the genetic variability of populations. The proposed project aims at the development of polymorphic microsatellite loci for the O. moratoi species as well as evaluation and comparison of the genetic variability of three populations recorded for this species (Ecological Station Itirapina, Botucatu and São Carlos), in order to verify the levels of genetic structure, inbreeding and gene flow, and relate the impact of the anthropogenic processes in genetic diversity. The results may provide measures for conservation programs and management of this endangered species. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MAURICIO P. ARRUDA; WILLIAM P. COSTA; SHIRLEI M. RECCO-PIMENTEL. Genetic diversity of Morato's Digger Toad, Proceratophrys moratoi: spatial structure, gene flow, effective size and the need for differential management strategies of populations. GENETICS AND MOLECULAR BIOLOGY, v. 40, n. 2, p. 502-514, Jun. 2017. Web of Science Citations: 0.
ARRUDA, MAURICIO PAPA; COSTA, WILLIAM PINHEIRO; SILVA, CARLA CRISTINA; RECCO PIMENTEL, SHIRLEI MARIA. Development of 22 Polymorphic Microsatellite Loci for the Critically Endangered Morato's Digger Toad, Proceratophrys moratoi. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 13, n. 10, p. 12259-12267, OCT 2012. Web of Science Citations: 3.

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