| Grant number: | 09/17275-7 |
| Support Opportunities: | Regular Research Grants |
| Start date: | March 01, 2010 |
| End date: | February 29, 2012 |
| Field of knowledge: | Health Sciences - Medicine - Maternal and Child Health |
| Principal Investigator: | Regina Célia de Menezes Succi |
| Grantee: | Regina Célia de Menezes Succi |
| Host Institution: | Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil |
Abstract
Concerned about the presence of co-infections between 2002-2003, we studied the response to vaccination against hepatitis A in children exposed to and infected by HIV (HIV) and exposed and HIV-uninfected (ENI). We observed that the rate of seroconversion after the second dose of vaccine was 100% for both groups of patients. Objective: To evaluate the 1-persistent levels of antibodies against hepatitis A in children previously vaccinated in the groups HIV and ENI. 2-To evaluate factors associated with the persistence of antibodies to hepatitis A vaccination induced by the group of children infected with HIV: HIV viral load and count of CD4 + T cells in the last six to eight years, immunophenotypic profile now. 3-In children who did not present levels of protective antibodies (<20 mIU / mL) in both groups re-vaccinated them with up to two additional doses and determine the antibody levels after each dose. Methods: 40 children will be studied that are in regular follow-up at the Care Center in the Department of Pediatric Infectious Diseases (CEADIPe) Federal University of São Paulo (UNIFESP), belonging to the following groups: 30 HIV-infected children and that 10 ENI already received in 2002-2003, two doses of vaccine against HAV. The total antibodies against hepatitis A virus will be detected and quantified in human plasma by a chemiluminescence immunoassay. The absolute numbers of various lymphocyte subsets detected by flow cytometry will be obtained from that total lymphocyte count. Immunophenotyping of lymphocytes will be performed to evaluate CD4 +, CD8 +, NK cells and B cells T cells, NK and B will also be evaluated for maturation and activation. Analysis of results: There will be comparisons: 1-The percentage of immune individuals and mean levels of antibodies to hepatitis A virus in both groups (HIV, ENI). 2 - The immunological profile assessed in the HIV vac. 3-HIV group will be assessed the behavior of the HIV viral load and the count of CD4 + T cells and clinical outcome during the period after the primary vaccination against HAV and maintenance of antibody titers against HAV. 4-Among children not immune against the virus of hepatitis A in two groups, will be evaluated for their response to revaccination. (AU)
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