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Dinamic of antibodies against hepatitis A virus (HAV) in HIV-infected and HIV-exposed uninfected children and its association with lymphocyte immunophenotyping six to eight years after immunization

Abstract

Background: Concerned about the presence of co-infections between 2002-2003, we studied the response to vaccination against hepatitis A in children exposed to and infected by HIV (HIV) and exposed and HIV-uninfected (ENI). We observed that the rate of seroconversion after the second dose of vaccine was 100% for both groups of patients. Objective: To evaluate the 1-persistent levels of antibodies against hepatitis A in children previously vaccinated in the groups HIV and ENI. 2-To evaluate factors associated with the persistence of antibodies to hepatitis A vaccination induced by the group of children infected with HIV: HIV viral load and count of CD4 + T cells in the last six to eight years, immunophenotypic profile now. 3-In children who did not present levels of protective antibodies (<20 mIU / mL) in both groups re-vaccinated them with up to two additional doses and determine the antibody levels after each dose. Methods: 40 children will be studied that are in regular follow-up at the Care Center in the Department of Pediatric Infectious Diseases (CEADIPe) Federal University of São Paulo (UNIFESP), belonging to the following groups: 30 HIV-infected children and that 10 ENI already received in 2002-2003, two doses of vaccine against HAV. The total antibodies against hepatitis A virus will be detected and quantified in human plasma by a chemiluminescence immunoassay. The absolute numbers of various lymphocyte subsets detected by flow cytometry will be obtained from that total lymphocyte count. Immunophenotyping of lymphocytes will be performed to evaluate CD4 +, CD8 +, NK cells and B cells T cells, NK and B will also be evaluated for maturation and activation. Analysis of results: There will be comparisons: 1-The percentage of immune individuals and mean levels of antibodies to hepatitis A virus in both groups (HIV, ENI). 2 - The immunological profile assessed in the HIV vac. 3-HIV group will be assessed the behavior of the HIV viral load and the count of CD4 + T cells and clinical outcome during the period after the primary vaccination against HAV and maintenance of antibody titers against HAV. 4-Among children not immune against the virus of hepatitis A in two groups, will be evaluated for their response to revaccination. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
AÍDA DE FÁTIMA THOMÉ BARBOSA GOUVÊA; MARIA ISABEL DE MORAES PINTO; MARISTELA MIYAMOTO; DAISY MARIA MACHADO; SILVANA DUARTE PESSOA; FABIANA BONONI DO CARMO; SUÊNIA CORDEIRO DE VASCONCELOS BELTRÃO; REGINA CÉLIA DE MENEZES SUCCI. Persistence of hepatitis A virus antibodies after primary immunization and response to revaccination in children and adolescents with perinatal HIV exposure. Revista Paulista de Pediatria, v. 33, n. 2, p. -, Jun. 2015.

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