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Periodontal treatment in controlled and uncontrolled Diabetic subjects: clinical, glycemic and cytokine and bone resorption related factors evaluations

Grant number: 08/09687-0
Support type:Regular Research Grants
Duration: February 01, 2009 - November 30, 2010
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal researcher:Poliana Mendes Duarte
Grantee:Poliana Mendes Duarte
Home Institution: Universidade de Guarulhos (UNG). Campus Guarulhos-Centro. Guarulhos , SP, Brazil

Abstract

The aims of this study will be: 1- to compare the levels of cytokines and bone resorption related factors (RANKL, OPG, TNF-alpha, IL-4, IFN-gamma, IL-23 and IL-17) in gingival crevicular fluid from type 2 diabetic subjects with chronic periodontitis before and at 3 and 6 months after non-surgical periodontal therapy; 2- to evaluate in these subjects and experimental times, the effects of this therapy on glycemic control (levels of glycated hemoglobin) and periodontal clinical parameters; 3- to correlate the glycemic, clinical and immunological parameters. Forty subjects with chronic periodontitis and type 2 diabetes mellitus will be included in this study. First, peripheral blood samples from all subjects will be analyzed for glycated hemoglobin. Based on the levels of glycated hemoglobin, the subjects will be divided into one of the following groups: Controlled diabetic subjects (n=20): glycated hemoglobin levels between 4.5% and 8%; Uncontrolled diabetic subjects (n=20): glycated hemoglobin levels higher than 8%. The subjects will be submitted to periodontal clinical examinations and gingival gingival fluid sampling to assess the total amounts and concentrations of RANKL, OPG, TNF-alpha, IL-4, IFN-gamma, IL-23 and IL-17 by ELISA. Subsequently, all subjects will be submitted to non-surgical periodontal therapy, including supragingival plaque control and scaling and root planing. Glycated hemoglobin, clinical and immunological evaluations will be also performed at 3 and 6 months after treatment. The intra-group comparisons (before and after treatment) for immunological, glycemic and clinical data will be sought by the Friedman test ou ANOVA. The inter-group comparisons (controlled/uncontrolled) will be sought by Mann-Whitney or t Student tests in all experimental times. Spearman's analysis was used to test the possible correlations between glycemic, clinical and immunological data. The significance level established for all analyses will be 5% (p< 0.05). (AU)

Scientific publications (9)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SANTOS, VANESSA R.; LIMA, JADSON A.; MIRANDA, TAMIRES S.; GONCALVES, TIAGO E. D.; FIGUEIREDO, LUCIENE C.; FAVERI, MARCELO; DUARTE, POLIANA M. Full-mouth disinfection as a therapeutic protocol for type-2 diabetic subjects with chronic periodontitis: Twelve-month clinical outcomes. A randomized controlled clinical trial. Journal of Clinical Periodontology, v. 40, n. 2, p. 155-162, FEB 2013. Web of Science Citations: 20.
MENDONCA, ADRIANA CUTRIM; SANTOS, VANESSA RENATA; RIBEIRO, FERNANDA VIEIRA; LIMA, JADSON ALMEIDA; MIRANDA, TAMIRES SZEREMESKE; FERES, MAGDA; DUARTE, POLIANA MENDES. Surgical and non-surgical therapy with systemic antimicrobials for residual pockets in type 2 diabetics with chronic periodontitis: a pilot study. Journal of Clinical Periodontology, v. 39, n. 4, p. 368-376, APR 2012. Web of Science Citations: 9.
SANTOS, V. R.; RIBEIRO, F. V.; LIMA, J. A.; MIRANDA, T. S.; FERES, M.; BASTOS, M. F.; DUARTE, P. M. Partial- and full-mouth scaling and root planing in type 2 diabetic subjects: a 12-mo follow-up of clinical parameters and levels of cytokines and osteoclastogenesis-related factors. JOURNAL OF PERIODONTAL RESEARCH, v. 47, n. 1, p. 45-54, FEB 2012. Web of Science Citations: 16.
NAPIMOGA, M. H.; NUNES, L. H. A. C.; MACIEL, A. A. B.; DEMASI, A. P. D.; BENATTI, B. B.; SANTOS, V. R.; BASTOS, M. F.; DE MIRANDA, T. S.; DUARTE, P. M. Possible Involvement of IL-21 and IL-10 on Salivary IgA Levels in Chronic Periodontitis Subjects. Scandinavian Journal of Immunology, v. 74, n. 6, p. 596-602, DEC 2011. Web of Science Citations: 9.
RIBEIRO, FERNANDA VIEIRA; DE MENDONCA, ADRIANA CUTRIM; SANTOS, VANESSA RENATA; BASTOS, MARTA FERREIRA; FIGUEIREDO, LUCIENE CRISTINA; DUARTE, POLIANA MENDES. Cytokines and Bone-Related Factors in Systemically Healthy Patients With Chronic Periodontitis and Patients With Type 2 Diabetes and Chronic Periodontitis. Journal of Periodontology, v. 82, n. 8, p. 1187-1196, AUG 2011. Web of Science Citations: 53.
DUARTE, POLIANA M.; SANTOS, VANESSA R.; DOS SANTOS, FERNANDA A.; DE LIMA PEREIRA, SANIVIA A.; RODRIGUES, DENISE B. R.; NAPIMOGA, MARCELO H. Role of Smoking and Type 2 Diabetes in the Immunobalance of Advanced Chronic Periodontitis. Journal of Periodontology, v. 82, n. 3, p. 429-438, MAR 2011. Web of Science Citations: 16.
SANTOS, VANESSA RENATA; RIBEIRO, FERNANDA VIEIRA; LIMA, JADSON ALMEIDA; NAPIMOGA, MARCELO HENRIQUE; BASTOS, MARTA FERREIRA; DUARTE, POLIANA MENDES. Cytokine levels in sites of chronic periodontitis of poorly controlled and well-controlled type 2 diabetic subjects. JOURNAL OF CLINICAL PERIODONTOLOGY, v. 37, n. 12, p. 1049-1058, DEC 2010. Web of Science Citations: 41.
SANTOS, VANESSA RENATA; LIMA, JADSON ALMEIDA; DIAS GONCALVES, TIAGO EDUARDO; BASTOS, MARTA FERREIRA; FIGUEIREDO, LUCIENE CRISTINA; SHIBLI, JAMIL AWAD; DUARTE, POLIANA MENDES. Receptor Activator of Nuclear Factor-Kappa B Ligand/Osteoprotegerin Ratio in Sites of Chronic Periodontitis of Subjects With Poorly and Well-Controlled Type 2 Diabetes. Journal of Periodontology, v. 81, n. 10, p. 1455-1465, OCT 2010. Web of Science Citations: 35.
DUARTE, POLIANA MENDES; DA ROCHA, MARCELO; SAMPAIO, EDUARDO; MESTNIK, MARIA JOSEFA; FERES, MAGDA; FIGUEIREDO, LUCIENE CRISTINA; BASTOS, MARTA FERREIRA; FAVERI, MARCELO. Serum Levels of Cytokines in Subjects With Generalized Chronic and Aggressive Periodontitis Before and After Non-Surgical Periodontal Therapy: A Pilot Study. Journal of Periodontology, v. 81, n. 7, p. 1056-1063, JUL 2010. Web of Science Citations: 68.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.