Molecular biology and proteomics of medically interesting fungi: Paracoccidioides brasiliensis and Sporothrix schenckii

• Grant number: 09/54024-2
• Support Opportunities: Research Projects - Thematic Grants
• Duration: November 01, 2010 - December 31, 2016
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Zoilo Pires de Camargo
• Grantee: Zoilo Pires de Camargo
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Pesquisadores principais: Leila Maria Lopes Bezerra ; Luciano dos Santos Feitosa
• Associated scholarship(s): 13/05405-9 - Analysis of genetic diversity in agents of paracoccidioidomycosis using AFLP (Amplified fragment length polymorphism), BP.MS; 12/06593-0 - Paracoccidioides lutzii: Antigenic and Molecular studies., BP.MS

Abstract

The fungi Paracoccidioides brasiliensis and Sporothrix schenckii are of a great interest in medical and biological areas. The first is the agent of paracoccidioidomycosis, an endemic disease in many regions of Latin America, mainly in Brazil. The second is the etiological agent of sporotrichosis, subcutaneous mycosis, a re-emergent disease in our environment, and nowadays constitutes a zoonosis, because once infects cats and they are transmitters to humans. The proteomics, cromossomics and biochemicals characteristics of these fungi are not so studied. Although, in our study we will focus these aspects in these two agents for a better understanding of its biology. In the S. schenckii complex we will look for molecular evidences of intra-specific variations, through proteomic differential analysis among the species S. brasiliensis, S. globosa, S. Mexicana and S. schenckii, availing the differentially expressed protein and also, through immunoproteomic to determine the immunogenic proteins in the fix and lymphocutaneous sporotrichosis types. Also, in relation to S. schenckii, the variation of chromosomal profiles from different strains will be studied, mapping some of gene. Proteomic profiles of different P. brasiliensis isolates will be studied, and through immunoproteomic, the reactive proteomic map related with recognizing specifics antibodies from patients on the stage of acute and chronic paracoccidioidomycosis will be determined, as well as the characterization of a calnexin by the detailed study of its cellular location during the dimorphic transition and after stress conditions. (AU)

Scientific publications

(26)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DO AMARAL, CRISTIANE CANDIDA; FERNANDES, GEISA FERREIRA; RODRIGUES, ANDERSON MESSIAS; BURGER, EVA; DE CAMARGO, ZOILO PIRES. Proteomic analysis of Paracoccidioides brasiliensis complex isolates: Correlation of the levels of differentially expressed proteins with in vivo virulence. PLoS One, v. 14, n. 7, JUL 2 2019. (09/54024-2, 17/27265-5)

RODRIGUES, ANDERSON MESSIAS; KUBITSCHEK-BARREIRA, PAULA H.; FERNANDES, GEISA FERREIRA; DE ALMEIDA, SANDRO ROGERIO; LOPES-BEZERRA, LEILA M.; DE CAMARGO, ZOILO PIRES. Two-dimensional gel electrophoresis data for proteomic profiling of Sporothrix yeast cells. DATA IN BRIEF, v. 2, p. 32-38, MAR 2015. (09/54024-2)

DE ARAUJO, MARICELI L.; RODRIGUES, ANDERSON M.; FERNANDES, GEISA F.; DE CAMARGO, ZOILO P.; DE HOOG, G. SYBREN. Human sporotrichosis beyond the epidemic front reveals classical transmission types in Espirito Santo, Brazil. MYCOSES, v. 58, n. 8, p. 485-490, AUG 2015. (11/07350-1, 09/54024-2)

DOS SANTOS, PRISCILA OLIVEIRA; RODRIGUES, ANDERSON MESSIAS; FERNANDES, GEISA FERREIRA; MARQUES DA SILVA, SILVIA HELENA; BURGER, EVA; DE CAMARGO, ZOILO PIRES. Immunodiagnosis of Paracoccidioidomycosis due to Paracoccidioides brasiliensis Using a Latex Test: Detection of Specific Antibody Anti-gp43 and Specific Antigen gp43. PLoS Neglected Tropical Diseases, v. 9, n. 2, FEB 2015. (11/07350-1, 09/54024-2)

HAHN, ROSANE CHRISTINE; RODRIGUES, ANDERSON MESSIAS; FERNANDES FONTES, COR JESUS; NERY, ANDREIA FERREIRA; TADANO, TOMOKO; QUEIROZ JUNIOR, LUIZ DE PADUA; DE CAMARGO, ZOILO PIRES. Case Report: Fatal Fungemia due to Paracoccidioides lutzii. American Journal of Tropical Medicine and Hygiene, v. 91, n. 2, p. 394-398, AUG 2014. (11/07350-1, 09/54024-2)

GEGEMBAUER, GREGORY; ARAUJO, LETICIA MENDES; PEREIRA, EDY FIRMINA; RODRIGUES, ANDERSON MESSIAS; MIRANDA PANIAGO, ANAMARIA MELLO; HAHN, ROSANE CHRISTINE; DE CAMARGO, ZOILO PIRES. Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii. PLoS Neglected Tropical Diseases, v. 8, n. 7, JUL 2014. (09/54024-2, 12/06593-0)

GOMPERTZ, OLGA FISCHMAN; RODRIGUES, ANDERSON M.; FERNANDES, GEISA F.; BENTUBO, HENRI D. L.; DE CAMARGO, ZOILO PIRES; PETRI, VALERIA. Case Report: Atypical Clinical Presentation of Sporotrichosis Caused by Sporothrix globosa Resistant to Itraconazole. American Journal of Tropical Medicine and Hygiene, v. 94, n. 6, p. 1218-1222, JUN 2016. (09/54024-2)

RODRIGUES, ANDERSON MESSIAS; DE HOOG, G. SYBREN; DE CAMARGO, ZOILO PIRES. Sporothrix Species Causing Outbreaks in Animals and Humans Driven by Animal-Animal Transmission. PLOS PATHOGENS, v. 12, n. 7, JUL 2016. (15/19746-8, 09/54024-2)

RODRIGUES, ANDERSON MESSIAS; FERNANDES, GEISA FERREIRA; ARAUJO, LETICIA MENDES; DELLA TERRA, PAULA PORTELLA; DOS SANTOS, PRISCILA OLIVEIRA; PEREIRA, SANDRO ANTONIO; PACHECO SCHUBACH, TANIA MARIA; BURGER, EVA; LOPES-BEZERRA, LEILA MARIA; DE CAMARGO, ZOILO PIRES. Proteomics-Based Characterization of the Humoral Immune Response in Sporotrichosis: Toward Discovery of Potential Diagnostic and Vaccine Antigens. PLoS Neglected Tropical Diseases, v. 9, n. 8, AUG 2015. (11/07350-1, 09/54024-2)

RODRIGUES, ANDERSON MESSIAS; SYBREN DE HOOG, G.; DE CAMARGO, ZOILO PIRES. Molecular Diagnosis of Pathogenic Sporothrix Species. PLoS Neglected Tropical Diseases, v. 9, n. 12, DEC 2015. (09/54024-2)

RODRIGUES, ANDERSON MESSIAS; DE HOOG, G. SYBREN; DE CAMARGO, ZOILO PIRES. Genotyping species of the Sporothrix schenckii complex by PCR-RFLP of calmodulin. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 78, n. 4, p. 383-387, APR 2014. (11/07350-1, 09/54024-2)

MONTENEGRO, HILDEBRANDO; RODRIGUES, ANDERSON MESSIAS; GALVAO DIAS, MARIA ADELAIDE; DA SILVA, ELISABETE APARECIDA; BERNARDI, FERNANDA; DE CAMARGO, ZOILO PIRES. Feline sporotrichosis due to Sporothrix brasiliensis: an emerging animal infection in Sao Paulo, Brazil. BMC Veterinary Research, v. 10, NOV 19 2014. (11/07350-1, 09/54024-2)

MARQUES-DA-SILVA, SILVIA HELENA; RODRIGUES, ANDERSON MESSIAS; SYBREN DE HOOG, G.; SILVEIRA-GOMES, FABIOLA; DE CAMARGO, ZOILO PIRES. Case Report: Occurrence of Paracoccidioides lutzii in the Amazon Region: Description of Two Cases. American Journal of Tropical Medicine and Hygiene, v. 87, n. 4, p. 710-714, OCT 2012. (11/07350-1, 09/54024-2)

FERNANDES, GEISA FERREIRA; DOS SANTOS, PRISCILA OLIVEIRA; RODRIGUES, ANDERSON MESSIAS; SASAKI, ALEXANDRE AUGUSTO; BURGER, EVA; DE CAMARGO, ZOILO PIRES. Characterization of virulence profile, protein secretion and immunogenicity of different Sporothrix schenckii sensu stricto isolates compared with S. globosa and S. brasiliensis species. VIRULENCE, v. 4, n. 3, p. 241-249, APR 2013. (09/54024-2)

RODRIGUES, ANDERSON M.; NAJAFZADEH, MOHAMMAD J.; DE HOOG, G. SYBREN; DE CAMARGO, ZOILO P.. Rapid Identification of Emerging Human-Pathogenic Sporothrix Species with Rolling Circle Amplification. FRONTIERS IN MICROBIOLOGY, v. 6, DEC 8 2015. (11/07350-1, 09/54024-2)

RODRIGUES, ANDERSON MESSIAS; KUBITSCHEK-BARREIRA, PAULA H.; FERNANDES, GEISA FERREIRA; DE ALMEIDA, SANDRO ROGERIO; LOPES-BEZERRA, LEILA M.; DE CAMARGO, ZOILO PIRES. Immunoproteomic analysis reveals a convergent humoral response signature in the Sporothrix schenckii complex. JOURNAL OF PROTEOMICS, v. 115, p. 8-22, FEB 6 2015. (09/54024-2)

RODRIGUES, ANDERSON MESSIAS; DE HOOG, SYBREN; DE CAMARGO, ZOILO PIRES. Emergence of pathogenicity in the Sporothrix schenckii complex. Medical Mycology, v. 51, n. 4, p. 405-412, MAY 2013. (11/07350-1, 09/54024-2)

RODRIGUES, ANDERSON MESSIAS; BAGAGLI, EDUARDO; DE CAMARGO, ZOILO PIRES; GIMENES BOSCO, SANDRA DE MORAES. Sporothrix schenckii sensu stricto Isolated from Soil in an Armadillo's Burrow. Mycopathologia, v. 177, n. 3-4, p. 199-206, APR 2014. (11/07350-1, 09/54024-2)

RODRIGUES, ANDERSON MESSIAS; CRUZ CHOAPPA, RODRIGO; FERNANDES, GEISA FERREIRA; DE HOOG, G. SYBREN; DE CAMARGO, ZOILO PIRES. Sporothrix chilensis sp nov (Ascomycota: Ophiostomatales), a soil-borne agent of human sporotrichosis with mild-pathogenic potential to mammals. FUNGAL BIOLOGY, v. 120, n. 2, SI, p. 246-264, FEB 2016. (09/54024-2)

RODRIGUES, ANDERSON MESSIAS; DE HOOG, G. SYBREN; PIRES, DEBORA DE CASSIA; NOGUEIRA BRIHANTE, RAIMUNDA SAMIA; DA COSTA SIDRIM, JOSE JULIO; GADELHA, MARCOS FABIO; COLOMBO, ARNALDO LOPES; DE CAMARGO, ZOILO PIRES. Genetic diversity and antifungal susceptibility profiles in causative agents of sporotrichosis. BMC INFECTIOUS DISEASES, v. 14, APR 23 2014. (09/54024-2)

TEIXEIRA, MARCUS DE MELO; RODRIGUES, ANDERSON MESSIAS; TSUI, CLEMENT K. M.; PAULO DE ALMEIDA, LUIZ GONZAGA; VAN DIEPENINGEN, ANNE D.; VAN DEN ENDE, BERT GERRITS; FERNANDES, GEISA FERREIRA; KANO, RUI; HAMELIN, RICHARD C.; LOPES-BEZERRA, LEILA MARIA; et al. Asexual Propagation of a Virulent Clone Complex in a Human and Feline Outbreak of Sporotrichosis. Eukaryotic Cell, v. 14, n. 2, p. 158-169, FEB 2015. (11/07350-1, 09/54024-2)

ROBERTO, THIAGO NUNES; RODRIGUES, ANDERSON MESSIAS; HAHN, ROSANE CHRISTINE; DE CAMARGO, ZOILO PIRES. Identifying Paracoccidioides phylogenetic species by PCR-RFLP of the alpha-tubulin gene. Medical Mycology, v. 54, n. 3, p. 240-247, MAR 2016. (11/07350-1, 13/05405-9, 09/54024-2)

NOGUEIRA BRILHANTE, RAIMUNDA SAMIA; RODRIGUES, ANDERSON MESSIAS; COSTA SIDRIM, JOSE JULIO; GADELHA ROCHA, MARCOS FABIO; PEREIRA, SANDRO ANTONIO; FERREIRA GREMIAO, ISABELLA DIB; PACHECO SCHUBACH, TANIA MARIA; DE CAMARGO, ZOILO PIRES. In vitro susceptibility of antifungal drugs against Sporothrix brasiliensis recovered from cats with sporotrichosis in Brazil. Medical Mycology, v. 54, n. 3, p. 275-279, MAR 2016. (11/07350-1, 09/54024-2)

RODRIGUES, ANDERSON MESSIAS; SYBREN DE HOOG, G.; ZHANG, YU; DE CAMARGO, ZOILO PIRES. Emerging sporotrichosis is driven by clonal and recombinant Sporothrix species. EMERGING MICROBES & INFECTIONS, v. 3, MAY 7 2014. (11/07350-1, 09/54024-2)

DELLA TERRA, PAULA PORTELLA; RODRIGUES, ANDERSON MESSIAS; FERNANDES, GEISA FERREIRA; NISHIKAKU, ANGELA SATIE; BURGER, EVA; DE CAMARGO, ZOILO PIRES. Exploring virulence and immunogenicity in the emerging pathogen Sporothrix brasiliensis. PLoS Neglected Tropical Diseases, v. 11, n. 8, AUG 2017. (15/19746-8, 09/54024-2)

HAHN, ROSANE CHRISTINE; RODRIGUES, ANDERSON MESSIAS; DELLA TERRA, PAULA PORTELLA; NERY, ANDREIA FERREIRA; HOFFMANN-SANTOS, HUGO DIAS; GOIS, HELLEN MEIRA; FERNANDES FONTES, COR JESUS; DE CAMARGO, ZOILO PIRES. Clinical and epidemiological features of paracoccidioidomycosis due to Paracoccidioides lutzii. PLoS Neglected Tropical Diseases, v. 13, n. 6, JUN 2019. (09/54024-2, 17/27265-5)