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Center for Structural Molecular Biotechnology

Grant number: 98/14138-2
Support type:Research Grants - Research, Innovation and Dissemination Centers - RIDC
Duration: October 01, 2000 - December 31, 2012
Field of knowledge:Interdisciplinary Subjects
Principal Investigator:Glaucius Oliva
Grantee:Glaucius Oliva
Home Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Co-Principal Investigators:Heloisa Sobreiro Selistre de Araújo ; Leila Maria Beltramini ; Otavio Henrique Thiemann ; Paulo Cézar Vieira ; Richard Charles Garratt ; Rogerio Meneghini
Associated grant(s):03/02879-8 - Structural characterization of jackin and frutakin, new chitin binding lectins, that inhibit fungi growing, AR.EXT
Associated scholarship(s):11/20863-8 - Functional studies of SECIS elements in Trypanossomatideos, BP.IC
11/23302-7 - Expression, purification and crystallization of a septins complex, BP.IC
10/19390-5 - Aging-related alterations in extracellular matrix and its adaptations to strength training in calcaneal tendon of rats: molecular, cellular, and biomechanical approach, BP.PD
+ associated scholarships 11/19886-3 - Heterologous production and functional characterization of a beta-fructocuranosidase identified in a cDNA library of Sphenophorus levis, a sugarcane pest, BP.IC
11/04537-3 - Understanding the structure of proteins for college students, in view of mental models, BP.MS
11/07165-0 - Effects of resistance training on the adipocytes of ovariectomized rats: morphometric analysis and gene expression of myostatin and its receptor, BP.IC
10/20290-5 - Structural studies of human septins complex, BP.PD
10/19493-9 - Cloning and expression of the disintegrin and Cys-rich domains of human ADAM9 (a disintegrin and Metalloproteinase)in Pichia Pastoris, BP.IC
10/12331-3 - Evaluation of Tryponocidal activity of auranofin against diferent DTU of Trypanosoma Cruzi, BP.IC
10/10201-5 - The role of ADAM9 on tumor spreading via lymphatic system: a possible pharmacological target, BP.DR
10/01024-2 - Prospection of integrin-binding proteins in the venom of Bothropos alternatus, BP.IC
09/17698-5 - Structural investigation of the bovine enzyme diacylglyceroltransferase1 (DGAT1) and its interaction with lipid systems, BP.PD
09/16901-1 - Study of the effect of alternagin-C on matrix metalloproteases (MMPs) expression in fibroblasts and breast tumor cells, BP.IC
09/15320-5 - Biological screening, identification and design of new anticancer candidates from natural products and synthetic compounds, BP.DR
09/06692-6 - Structural and kinetic studies of the enzymes adenosine kinase and Hypoxanthine-guanine phosphoribosyltranferase from Schistosoma mansoni, BP.MS
09/00918-2 - Mutagenesis of the human septins and analysis of their interaction with other septins, BP.IC
08/09852-1 - Formulation and application of solubilization kits for proteins for structural studies by nuclear magnetic resonance, BP.IC
08/07676-1 - Structural and functional studies of the enzymes SsuD and SsuE of the alkane sulfonate transport system of Xanthomonas axonopodis pv.citri, BP.MS
08/09442-8 - Interaction between the human proteins SEPT3 and PM-Scl75/hRrp45: analysis of the connection between septin filaments and the exosome complex, BP.IC
07/06564-2 - Study of the interaction between the lectins pulchelin (RIP-2, Abrus puchellus), frutalin (Artocarpus incisa) and camptosemin (camptosema ellipticum) with biomembrane model systems, BP.PD
06/60279-5 - Molecular & structural biology and spectroscopy of septins, BP.PD
06/57573-9 - Biochemical and structural studies of human SEPT3 and SEPT5 septins and identification of protein partners , BP.DR
06/01534-5 - Studies of the interactions of thyroid hormone receptors with DNA and the coregulator proteins using X-ray crystallography, BP.PD
06/51973-5 - Structural studies of the GTPase domain isolated from human SEPT4 septin , BP.MS
06/53355-7 - Biochemical and structural studies of human SEPT2 septin: determining factors of the aggregates formation, BP.DD
04/10245-1 - Study of natural products seeking specific inhibitors of cysteine proteases, BP.PD
03/12588-0 - Study for obtaining bioactive natural products from plants, BP.DD
03/06895-8 - Center for Structural Molecular Biotechnology, BP.TT - associated scholarships

Abstract

The Center for Structural Molecular Biotechnology (CBME) is a joint initiative resulting from existing collaborative research projects involving: the (I) Laboratory of Protein Crystallography and Structural Biology of the Institute of Physics of Sao Carlos (IFSC), University of Sao Paulo; (II) the National Synchrotron Light Laboratory (LNLS), Campinas; (III) the Laboratory of Natural Products and Organic Synthesis of the Department of Chemistry, Federal University of Sao Carlos (DQ-UFSCar); (IV) researchers from the Department of Genetics and Evolution (DGE-UFSCar) and Department of Physiological Sciences (DCF-UFSCar). The major goal of this Center is to perform both applied and basic research as well as technological development in all areas of biotechnology that depend on Structure Based Molecular Design, specifically in the rational design of new structure-based compounds (drugs, vaccines, pesticides, herbicides) and in protein engineering. In order to achieve this goal the CBME promotes an integrated multidisciplinary approach including the application of the techniques of Molecular Biology, Biochemistry, Structural Biology (Protein Crystallography, Multidimensional NMR, Spectroscopy, Molecular Modeling and Bioinformatics), Medicinal Chemistry based on both Synthetic and Natural Product Chemistry, Molecular Immunology, Cell Biology and Pharmacology. The projects undertaken by the CBME are selected on the basis of social, industrial and medical demand. Maximum integration and collaboration with the private sector is always sought, particularly with pharmaceutical and biotechnology companies and research institutes within the health and agricultural sectors. The integration of biological sciences with the unique facilities of the LNLS represents a major advantage for the Center. The Brazilian pharmaceutical market is currently worth US$12 billion a year and yet there is no proprietary drug that has ever been developed within the country. The dependence on foreign technology will become critical in sensitive areas such as human health, agriculture and the environment. Areas which are specifically of national interest to Brazil and which are socially highly sensitive as they affect millions of individuals, such as infections tropical diseases for example, run the risk of becoming totally neglected in the research and development priorities of international industry. In direct contrast, Brazil currently possesses one of the richest natural sources of incalculable potential wealth in the form of its biodiversity, up until now the principal source of lead compounds used in drug development. The scope of the activities of the CBME is to contribute to the bridging of this gap, with an integrated research program involving biologists, physicists, chemists, experts in bioinformatics, molecular modeling, pharmacologists, etc…,focused on the elucidation of macromolecular structure and function and its application to the development of useful compounds or newly engineered proteins. Rational Structure Based Drug Design is nowadays the most efficient and cost-effective technology for the development of new drugs, capable of contributing at all stages of the process, from the discovery of new lead compounds, their optimization (in terms of affinity, specificity, efficacy, side-effects) and their approval by the relevant bodies. It is a methodology that is based on the inhibition or stimulation of the biological activity of macromolecules, proteins or nucleic acids (DNA and RNA), responsible for different diseases. The three-dimensional structural information on target molecules permits the discovery and synthesis of complementary compounds which may become potent drugs specifically directed at the targeted disease. Without exception, all of the largest international pharmaceutical companies nowadays include research and development divisions which employ such technology. Recently developed drugs such as the HIV protease inhibitors are a clear example of the power of this technology. This is an intrinsically interdisciplinary science. Once the target disease, parasite or molecular genetics factor responsible has been selected, an integrated effort involving different specialized researchers is required for the discovery of a new drug: Molecular Biology (cloning and expression of the recombinant target proteins); Biochemistry (purification and characterization of the targets); Crystallography (crystallization, synchrotron data collection, structure determination); NMR (high resolution solution studies); Spectroscopic techniques (EPR, CD, fluorescence, FTIR); Drug design (docking, de novo design, theoretical calculations); Medicinal Chemistry (synthesis of designed compounds, search for leads in natural products); Pharmacology (in vitro and in vivo biological assays of potential compounds, activity and toxicity tests, optimal doses, efficacy, side-effects, etc...). The above process is not necessarily linear; frequently it is necessary that many stages are repeated in a cyclical fashion. However, the possibility to visualize accurately the target site of the drug and of complexes of intermediate compounds can lead rapidly to the convergence of the drug design process, with all the advantages previously mentioned: speed, low relative cost, greater specific activity and as a consequence a reduction in side-effects. Similarly, in the international context, the modern biological sciences have become characterized by the interdisciplinary nature of the theoretical and experimental approaches employed. In all of the large research groups of the world one can readily recognize the total integration between specific researches areas involved in Structural Biology. In Brazil, one can still see a marked segregation among these areas, with the sporadic collaboration that exists limited by the very structure of the university departments. The establishment of an inter-institutional research nucleus, dedicated to Structural Molecular Biotechnology, unique within the country and even within Latin America, which could count on the participation of researchers from different institutions, centre on common projects chosen on the basis, of demand, including a strong interaction and partnership with the production sector, would be the greatest benefit of the centre. With the implementation of a patent law for pharmaceutical and biotechnological products in Brazil, in effect since May/1997, strong interest from the industry in establishing collaborative research projects centered on the development of new compounds is emerging. One example is a research project currently being undertaken by the IFSC group in conjunction with and financed by the company Eurofarma Laboratorios Ltda (2nd in the national ranking), entitled ‘Definition of strategies for research and development of new drugs in Brazil’. What is already clear in this study is that the academic community has to take the initiative in establishing the connection university-industry, with an active role in the prospection of realistic projects for industrial investment. Therefore, the CBME plans to select its projects based on demand and actively pursue integration with industry. The final goal is always the complete transfer of the technology developed to the industrial sector, while naturally respecting intellectual property rights and community ownership of natural products when applicable. On the educational front, the Center will benefit from the strong training programs for students and researchers in the area of Structural Biology, including undergraduate and graduate research training, in all institutions involved. Furthermore, the Center will closely work with the Center for Scientific and Cultural Diffusion, the arm of the university in Sao Carlos for interaction with the community, through strong programs directed towards high school students, further education of school teachers, the extension of libraries of experiments for school demonstrations, education at a distance via the Internet, videos, science fairs, lectures etc. The LNLS has also several activities devoted to the community, including courses, lectures, guided visits and summer schools. This will all contribute to a better understanding of the importance of molecular biology, genetic engineering and biotechnology, key scientific areas for the next century. The research groups involved have demonstrated their capacity for collaborative research in the field, with publications, PhD and MSc theses, and above all state of the art facilities which represent the core of the center. The LNLS represents a US$ 50 million counterpart investment; the IFSC/USP has had an investment of about US$ 3 million in its facilities over the past 8 years; the groups from UFSCar have well established laboratories. The LNLS has approved the construction of a new building to accommodate the whole of their biological research program. The IFSC group has over 1500 m2 of continuous lab. space, including a new area of about 500 m2 allocated for expansion within the next 6 months which will accommodate much of the new equipment envisaged. The salary of staff researchers and technicians, administrative support and general infrastructure is also considerable. (AU)

Articles published in Pesquisa FAPESP Maganize about the research grant:
The essence of molecules 
A promising forecast 
The essence of molecules 

Scientific publications (53)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
QUEIROZ DE CAVALHO, JULIO CESAR; BELTRAMINI, LEILA MARIA; SEGNINI BOSSOLAN, NELMA REGINA. Using a board game to teach protein synthesis to high school students. JOURNAL OF BIOLOGICAL EDUCATION, v. 53, n. 2, p. 205-216, MAR 15 2019. Web of Science Citations: 0.
HABRYLO, OLIVIER; EVANGELISTA, DANILO ELTON; CASTILHO, PRISCILA VASQUES; PELLOUX, JEROME; HENRIQUE-SILVA, FLAVIO. The pectinases from Sphenophorus levis: Potential for biotechnological applications. International Journal of Biological Macromolecules, v. 112, p. 499-508, JUN 2018. Web of Science Citations: 1.
SANTIAGO, A. C.; KHAN, Z. N.; MIGUEL, M. C.; GIRONDA, C. C.; SOARES-COSTA, A.; PELA, V. T.; LEITE, A. L.; EDWARDSON, J. M.; BUZALAF, M. A. R.; HENRIQUE-SILVA, F. A New Sugarcane Cystatin Strongly Binds to Dental Enamel and Reduces Erosion. JOURNAL OF DENTAL RESEARCH, v. 96, n. 9, p. 1051-1057, AUG 2017. Web of Science Citations: 3.
SCHNEIDER, VANESSA KARINE; SOARES-COSTA, ANDREA; CHAKRAVARTHI, MOHAN; RIBEIRO, CAROLINA; CHABREGAS, SABRINA MOUTINHO; FALCO, MARIA CRISTINA; HENRIQUE-SILVA, FLAVIO. Transgenic sugarcane overexpressing CaneCPI-1 negatively affects the growth and development of the sugarcane weevil Sphenophorus levis. Plant Cell Reports, v. 36, n. 1, p. 193-201, JAN 2017. Web of Science Citations: 4.
ALVES DA SILVA, MARCO TULIO; SILVA-JARDIM, IZALTINA; PORTAPILLA, GISELE BULHOES; ALVARES DE LIMA, GUSTAVO MACHADO; COSTA, FERNANDA CRISTINA; ANIBAL, FERNANDA DE FREITAS; THIEMANN, OTAVIO HENRIQUE. In vivo and in vitro auranofin activity against Trypanosoma cruzi: Possible new uses for an old drug. Experimental Parasitology, v. 166, p. 189-193, JUL 2016. Web of Science Citations: 7.
EVANGELISTA, DANILO ELTON; PEREIRA DE PAULA, FERNANDO FONSECA; RODRIGUES, ANDRE; HENRIQUE-SILVA, FLAVIO. Pectinases From Sphenophorus levis Vaurie, 1978 (Coleoptera: Curculionidae): Putative Accessory Digestive Enzymes. JOURNAL OF INSECT SCIENCE, v. 15, FEB 11 2015. Web of Science Citations: 10.
PEDEZZI, RAFAEL; FONSECA, FERNANDO P. P.; SANTOS JUNIOR, CELIO DIAS; KISHI, LUCIANO T.; TERRA, WALTER R.; HENRIQUE-SILVA, FLAVIO. A novel beta-fructofuranosidase in Coleoptera: Characterization of a beta-fructofuranosidase from the sugarcane weevil, Sphenophorus levis. Insect Biochemistry and Molecular Biology, v. 55, p. 31-38, DEC 2014. Web of Science Citations: 8.
PEREIRA DE PAULA, FERNANDO FONSECA; RIBEIRO, JULIANA UEMA; SANTOS, LIVIA MARA; FERREIRA DE SOUZA, DULCE HELENA; LEONARDECZ, EDUARDO; HENRIQUE-SILVA, FLAVIO; SELISTRE-DE-ARAUJO, HELOISA SOBREIRO. Molecular characterization of metalloproteases from Bothrops alternatus snake venom. Comparative Biochemistry and Physiology D-Genomics & Proteomics, v. 12, p. 74-83, DEC 2014. Web of Science Citations: 2.
DA SILVA, M. T. A.; SILVA-JARDIM, I.; THIEMANN, O. H. Biological Implications of Selenium and its Role in Trypanosomiasis Treatment. Current Medicinal Chemistry, v. 21, n. 15, p. 1772-1780, MAY 2014. Web of Science Citations: 12.
DE SOUZA, MARCOS MICHEL; MANZINE, LIVIA REGINA; DA SILVA, MARCOS VINICIUS G.; BETTINI, JEFFERSON; PORTUGAL, RODRIGO VILARES; CRUZ, ANGELA KAYSEL; ARRUDA, EURICO; THIEMANN, OTAVIO HENRIQUE. An improved purification procedure for Leishmania RNA virus (LRV). Brazilian Journal of Microbiology, v. 45, n. 2, p. 695-698, APR-JUN 2014. Web of Science Citations: 1.
SOARES-COSTA, ANDREA; NAKAYAMA, DARLAN GONCALVES; ANDRADE, LETICIA DE FREITAS; CATELLI, LUCAS FERIOLI; GUARNIERI BASSI, ANA PAULA; CECCATO-ANTONINI, SANDRA REGINA; HENRIQUE-SILVA, FLAVIO. Industrial PE-2 strain of Saccharomyces cerevisiae: from alcoholic fermentation to the production of recombinant proteins. NEW BIOTECHNOLOGY, v. 31, n. 1, p. 90-97, JAN 25 2014. Web of Science Citations: 0.
FAIM, LIVIA MARIA; ROSA E SILVA, IVAN; BERTACINE DIAS, MARCIO VINICIUS; PEREIRA, HUMBERTO D'MUNIZ; BRANDAO-NETO, JOSE; ALVES DA SILVA, MARCO TULIO; THIEMANN, OTAVIO HENRIQUE. Crystallization and preliminary X-ray diffraction analysis of selenophosphate synthetases from Trypanosoma brucei and Leishmania major. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, v. 69, n. 8, p. 864-867, AUG 2013. Web of Science Citations: 1.
MANZINE, LIVIA REGINA; BALASCO SERRAO, VITOR HUGO; TRAMBAIOLI DA ROCHA E LIMA, LUIS MAURICIO; DE SOUZA, MARCOS MICHEL; BETTINI, JEFFERSON; PORTUGAL, RODRIGO VILLARES; VAN HEEL, MARIN; THIEMANN, OTAVIO HENRIQUE. Assembly stoichiometry of bacterial selenocysteine synthase and SelC (tRNA(sec)). FEBS Letters, v. 587, n. 7, p. 906-911, APR 2 2013. Web of Science Citations: 4.
DA SILVA, M. T. A.; CALDAS, V. E. A.; COSTA, F. C.; SILVESTRE, D. A. M. M.; THIEMANN, O. H. Selenocysteine biosynthesis and insertion machinery in Naegleria gruberi. Molecular and Biochemical Parasitology, v. 188, n. 2, p. 87-90, APR 2013. Web of Science Citations: 8.
MACEDO, JOCI N. A.; VALADARES, NAPOLEAO F.; MARQUES, IVO A.; FERREIRA, FREDERICO M.; DAMALIO, JULIO C. P.; PEREIRA, HUMBERTO M.; GARRATT, RICHARD C.; ARAUJO, ANA P. U. The structure and properties of septin 3: a possible missing link in septin filament formation. Biochemical Journal, v. 450, n. 1, p. 95-105, FEB 15 2013. Web of Science Citations: 19.
LEITE, NEY RIBEIRO; FARO, ALINE REGIS; OLIVA DOTTA, MARIA AMELIA; FAIM, LIVIA MARIA; GIANOTTI, ANDREIA; SILVA, FLAVIO HENRIQUE; OLIVA, GLAUCIUS; THIEMANN, OTAVIO HENRIQUE. The crystal structure of the cysteine protease Xylellain from Xylella fastidiosa reveals an intriguing activation mechanism. FEBS Letters, v. 587, n. 4, p. 339-344, FEB 14 2013. Web of Science Citations: 2.
MARQUES, IVO DE ALMEIDA; ROMANELLO, LARISSA; DEMARCO, RICARDO; PEREIRA, HUMBERTO D'MUNIZ. Structural and kinetic studies of Schistosoma mansoni adenylate kinases. Molecular and Biochemical Parasitology, v. 185, n. 2, p. 157-160, OCT 2012. Web of Science Citations: 10.
SANTOS-SILVA, LUDIER K.; SOARES-COSTA, ANDREA; GERALD, LEE T. S.; MENEGHIN, SILVANA P.; HENRIQUE-SILVA, FLAVIO. Recombinant expression and biochemical characterization of sugarcane legumain. Plant Physiology and Biochemistry, v. 57, p. 181-192, AUG 2012. Web of Science Citations: 11.
FONSECA, FERNANDO P. P.; SOARES-COSTA, ANDREA; RIBEIRO, ALBERTO F.; ROSA, JOSE CESAR; TERRA, WALTER R.; HENRIQUE-SILVA, FLAVIO. Recombinant expression, localization and in vitro inhibition of midgut cysteine peptidase (Sl-CathL) from sugarcane weevil, Sphenophorus levis. Insect Biochemistry and Molecular Biology, v. 42, n. 1, p. 58-69, JAN 2012. Web of Science Citations: 8.
SOARES-COSTA, A.; SILVEIRA, R. S.; NOVO, M. T. M.; ALVES, M. F. M.; CARMONA, A. K.; BELASQUE, JR., J.; HENRIQUE-SILVA, F. Recombinant expression and characterization of a cysteine peptidase from Xanthomonas citri subsp citri. Genetics and Molecular Research, v. 11, n. 4, p. 4043-4057, 2012. Web of Science Citations: 3.
BALASCO SERRAO, VITOR HUGO; ALESSANDRO, FERNANDO; ARMINI CALDAS, VICTOR EMANOEL; MARCAL, RAFAELA LEITE; PEREIRA, HUMBERTO D'MUNIZ; THIEMANN, OTAVIO HENRIQUE; GARRATT, RICHARD CHARLES. Promiscuous interactions of human septins: The GTP binding domain of SEPT7 forms filaments within the crystal. FEBS Letters, v. 585, n. 24, p. 3868-3873, DEC 15 2011. Web of Science Citations: 14.
COSTA, F. C.; OLIVA, M. A. V.; DE JESUS, T. C. L.; SCHENKMAN, S.; THIEMANN, O. H. Oxidative stress protection of Trypanosomes requires selenophosphate synthase. Molecular and Biochemical Parasitology, v. 180, n. 1, p. 47-50, NOV 2011. Web of Science Citations: 8.
FONSECA, FERNANDO P. P.; IKE, PRISCILA T. L.; ASSIS, DIEGO M.; ICIMOTO, MARCELO Y.; JULIANO, MARIA A.; JULIANO, LUIZ; PUZER, LUCIANO; HENRIQUE-SILVA, FLAVIO. Leviserpin: A Serine Peptidase Inhibitor (Serpin) from the Sugarcane Weevil Sphenophorus levis. The Protein Journal, v. 30, n. 6, p. 404-412, AUG 2011. Web of Science Citations: 4.
GIUSEPPE, PRISCILA O.; VON ATZINGEN, MARINA; NASCIMENTO, ANA LUCIA T. O.; ZANCHIN, NILSON I. T.; GUIMARAES, BEATRIZ G. The crystal structure of the leptospiral hypothetical protein LIC12922 reveals homology with the periplasmic chaperone SurA. Journal of Structural Biology, v. 173, n. 2, p. 312-322, FEB 2011. Web of Science Citations: 6.
MORELLO, LUIS G.; HESLING, CEDRIC; COLTRI, PATRICIA P.; CASTILHO, BEATRIZ A.; RIMOKH, RUTH; ZANCHIN, NILSON I. T. The NIP7 protein is required for accurate pre-rRNA processing in human cells. Nucleic Acids Research, v. 39, n. 2, p. 648-665, JAN 2011. Web of Science Citations: 14.
BLEICHER, LUCAS; LEMKE, NEY; GARRATT, RICHARD CHARLES. Using Amino Acid Correlation and Community Detection Algorithms to Identify Functional Determinants in Protein Families. PLoS One, v. 6, n. 12, p. e27786, 2011. Web of Science Citations: 18.
RINKE, R.; COSTA, A. S.; FONSECA, F. P. P.; ALMEIDA, L. C.; DELALIBERA JUNIOR, I.; HENRIQUE-SILVA, F. Microbial diversity in the larval gut of field and laboratory populations of the sugarcane weevil Sphenophorus levis (Coleoptera, Curculionidae). Genetics and Molecular Research, v. 10, n. 4, p. 2679-2691, 2011. Web of Science Citations: 12.
VALADARES, NAPOLEAO F.; DELLAMANO, MARCIA; SOARES-COSTA, ANDREA; HENRIQUE-SILVA, FLAVIO; GARRATT, RICHARD C. Molecular determinants of improved cathepsin B inhibition by new cystatins obtained by DNA shuffling. BMC STRUCTURAL BIOLOGY, v. 10, SEP 30 2010. Web of Science Citations: 4.
DOMINGUES, MARIANE NORONHA; DE SOUZA, TIAGO ANTONIO; CERNADAS, RAUL ANDRES; PEIXOTO DE OLIVEIRA, MARIA LUIZA; DOCENA, CASSIA; FARAH, CHUCK SHAKER; BENEDETTI, CELSO EDUARDO. The Xanthomonas citri effector protein PthA interacts with citrus proteins involved in nuclear transport, protein folding and ubiquitination associated with DNA repair. MOLECULAR PLANT PATHOLOGY, v. 11, n. 5, p. 663-675, SEP 2010. Web of Science Citations: 23.
CORDEIRO, ARTUR T.; THIEMANN, OTAVIO H. 16-Bromoepiandrosterone, an activator of the mammalian immune system, inhibits glucose 6-phosphate dehydrogenase from Trypanosoma cruzi and is toxic to these parasites grown in culture. Bioorganic & Medicinal Chemistry, v. 18, n. 13, p. 4762-4768, JUL 1 2010. Web of Science Citations: 19.
SOUZA, T. A. C. B.; BARBOSA, J. A. R. G. Cloning, Overexpression, Purification and Preliminary Characterization of Human Septin 8. The Protein Journal, v. 29, n. 5, p. 328-335, JUL 2010. Web of Science Citations: 1.
PEREIRA, HUMBERTO M.; BERDINI, VALERIO; FERRI, MARIANA R.; CLEASBY, ANNE; GARRATT, RICHARD C. Crystal structure of Schistosoma purine nucleoside phosphorylase complexed with a novel monocyclic inhibitor. Acta Tropica, v. 114, n. 2, p. 97-102, MAY 2010. Web of Science Citations: 14.
DE OLIVEIRA, JULIANA FERREIRA; SFORCA, MAURICIO L.; BLUMENSCHEIN, THARIN M. A.; GOLDFEDER, MAURICIO B.; GUIMARAES, BEATRIZ G.; OLIVEIRA, CARLA COLUMBANO; ZANCHIN, NILSON I. T.; ZERI, ANA-CAROLINA. Structure, Dynamics, and RNA Interaction Analysis of the Human SBDS Protein. Journal of Molecular Biology, v. 396, n. 4, p. 1053-1069, MAR 5 2010. Web of Science Citations: 17.
AKAO, P. K.; TONOLI, C. C. C.; NAVARRO, M. S.; CINTRA, A. C. O.; NETO, J. R.; ARNI, R. K.; MURAKAMI, M. T. Structural studies of BmooMP alpha-I, a non-hemorrhagic metalloproteinase from Bothrops moojeni venom. Toxicon, v. 55, n. 2-3, p. 361-368, FEB-MAR 2010. Web of Science Citations: 25.
PEREIRA, HUMBERTO M.; REZENDE, MARTHA M.; CASTILHO, MARCELO SANTOS; OLIVA, GLAUCIUS; GARRATT, RICHARD C. Adenosine binding to low-molecular-weight purine nucleoside phosphorylase: the structural basis for recognition based on its complex with the enzyme from Schistosoma mansoni. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, v. 66, n. 1, p. 73-79, JAN 2010. Web of Science Citations: 14.
VALADARES, NAPOLEAO F.; SALUM, LIVIA B.; POLIKARPOV, IGOR; ANDRICOPULO, ADRIANO D.; GARRATT, RICHARD C. Role of Halogen Bonds in Thyroid Hormone Receptor Selectivity: Pharmacophore-Based 3D-QSSR Studies. JOURNAL OF CHEMICAL INFORMATION AND MODELING, v. 49, n. 11, p. 2606-2616, NOV 2009. Web of Science Citations: 35.
CERNADAS, RAUL ANDRES; BENEDETTI, CELSO EDUARDO. Role of auxin and gibberellin in citrus canker development and in the transcriptional control of cell-wall remodeling genes modulated by Xanthomonas axonopodis pv. citri. Plant Science, v. 177, n. 3, p. 190-195, SEP 2009. Web of Science Citations: 24.
DE OLIVEIR, JULIANA FERREIRA; CASTILHO, BEATRIZ A.; SFORCA, MAURICIO L.; KRIEGER, MARCO AURELIO; ZERI, ANA CAROLINA; GUIMARAES, BEATRIZ G.; ZANCHIN, NILSON I. T. Characterization of the Trypanosoma cruzi ortholog of the SBDS protein reveals an intrinsically disordered extended C-terminal region showing RNA-interacting activity. Biochimie, v. 91, n. 4, p. 475-483, APR 2009. Web of Science Citations: 2.
CORDEIRO, ARTUR T.; THIEMANN, OTAVIO H.; MICHELS, PAUL A. M. Inhibition of Trypanosoma brucei glucose-6-phosphate dehydrogenase by human steroids and their effects on the viability of cultured parasites. Bioorganic & Medicinal Chemistry, v. 17, n. 6, p. 2483-2489, MAR 15 2009. Web of Science Citations: 35.
MESQUITA-FERRARI, R. A.; DE MORAES, C. K.; MICOCCI, K. C.; SELISTRE-DE-ARAUJO, H. S. ALT-C, A DISINTEGRIN-LIKE CYS-RICH PROTEIN FROM Bothrops alternatus, INCREASES SKELETAL MYOBLAST VIABILITY. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 15, n. 2, p. 325-339, 2009. Web of Science Citations: 6.
RINALDI, FABIO C.; MEZA, ANDREIA N.; GUIMARAES, BEATRIZ G. Structural and Biochemical Characterization of Xylella fastidiosa DsbA Family Members: New Insights into the Enzyme-Substrate Interaction. BIOCHEMISTRY, v. 48, n. 15, p. 3508-3518, 2009. Web of Science Citations: 14.
CERNADAS, RAUL ANDRES; CAMILLO, LUCIANA RODRIGUES; BENEDETTI, CELSO EDUARDO. Transcriptional analysis of the sweet orange interaction with the citrus canker pathogens Xanthomonas axonopodis pv. citri and Xanthomonas axonopodis pv. aurantifolii. MOLECULAR PLANT PATHOLOGY, v. 9, n. 5, p. 609-631, SEP 2008. Web of Science Citations: 52.
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Please report errors in scientific publications list by writing to: cdi@fapesp.br.
Filed patent(s) as a result of this research project

MODELO TRIDIMENSIONAL PARA REPRESENTAR MOLÉCULA OU PARTE DE MOLÉCULA DE ÁCIDO NUCLÉICO E KIT PI0301512-2 - Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) ; Universidade de São Paulo (USP) . Leila Maria Beltramini; Ana Paula Ulian de Araujo; Luciano Douglas dos Santos Abel - May 2003, 16

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