Identification and localization of ²-lactam and tetracycline resistance genes, in clinical and environmental Enterobacteriaceae and Aeromonas hydrophila strains

• Grant number: 10/12841-1
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2010
• End date: October 31, 2012
• Field of knowledge: Health Sciences - Collective Health - Public Health
• Principal Investigator: Maria Helena Matte
• Grantee: Maria Helena Matte
• Host Institution: Faculdade de Saúde Pública (FSP). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Glavur Rogerio Matté ; Milena Dropa

Abstract

Antimicrobial resistance, a global public health problem, is facilitated by the selective pressure of antimicrobial use in clinical infections and other activities, as agriculture and animal feeding. The main way of resistance spread is the horizontal gene transfer between bacterial cells, by mobile genetic elements. Objective. To identify and characterize the genetic environment and the mobilization means of ²-lactam and tetracycline resistance genes in Enterobacteriaceae and Aeromonas hydrophila from clinical and environmental sources. Material and Methods. The study begins with 164 clinical and 85 environmental strains, which will be screened for ²-lactam and tetracycline phenotypic resistance, and for the presence of the respective resistance genes by PCR and sequencing. After their identification, the resistance genes will be investigated in regard to their plasmidial or chromosomal location, through pulsed field electrophoresis followed by hybridization, and the genetic environment will be searched by integron and transposon mapping by PCR. Expected Results. This study intends to determine the phenotypic ²-lactam and tetracycline resistance characteristics of strains, and also to define the identification and localization of the related antimicrobial resistance genes. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DROPA, MILENA; BALSALOBRE, LIVIA C.; LINCOPAN, NILTON; MATTE, GLAVUR R.; MATTE, MARIA H.. Complex class 1 integrons harboring CTX-M-2-encoding genes in clinical Enterobacteriaceae from a hospital in Brazil. JOURNAL OF INFECTION IN DEVELOPING COUNTRIES, v. 9, n. 8, p. 890-897, AUG 2015. (10/12841-1, 08/08312-3)

DROPA, MILENA; GHIGLIONE, BARBARA; MATTE, MARIA HELENA; BALSALOBRE, LIVIA CARMINATO; LINCOPAN, NILTON; MATTE, GLAVUR ROGERIO; GUTKIND, GABRIEL; POWER, PABLO. Molecular and Biochemical Characterization of CTX-M-131, a Natural Asp240Gly Variant Derived from CTX-M-2, Produced by a Providencia rettgeri Clinical Strain in Sao Paulo, Brazil. Antimicrobial Agents and Chemotherapy, v. 59, n. 3, p. 1815-1817, MAR 2015. (13/07943-8, 10/12841-1, 08/08312-3)

DROPA, MILENA; LINCOPAN, NILTON; BALSALOBRE, LIVIA C.; OLIVEIRA, DANIELLE E.; MOURA, RODRIGO A.; FERNANDES, MIRIAM RODRIGUEZ; DA SILVA, QUEZIA MOURA; MATTE, GLAVUR R.; SATO, MARIA I. Z.; MATTE, MARIA H.. Genetic background of novel sequence types of CTX-M-8-and CTX-M-15-producing Escherichia coli and Klebsiella pneumoniae from public wastewater treatment plants in Sao Paulo, Brazil. Environmental Science and Pollution Research, v. 23, n. 5, p. 4953-4958, MAR 2016. (10/12841-1)

BALSALOBRE, LIVIA CARMINATO; DROPA, MILENA; MATTE, MARIA HELENA. An overview of antimicrobial resistance and its public health significance. Brazilian Journal of Microbiology, v. 45, n. 1, p. 1-5, 2014. (10/08695-0, 10/12841-1)