Standardized herbal medicines for the treatment of chronic diseases

• Grant number: 09/52237-9
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• Duration: April 01, 2011 - March 31, 2016
• Field of knowledge: Physical Sciences and Mathematics - Chemistry
• Principal Investigator: Wagner Vilegas
• Grantee: Wagner Vilegas
• Host Institution: Instituto de Biociências (IB-CLP). Universidade Estadual Paulista (UNESP). Campus Experimental do Litoral Paulista. São Vicente , SP, Brazil
• Pesquisadores principais: Clélia Akiko Hiruma Lima
• Associated scholarship(s): 15/21479-8 - Standardization of plant extracts of Mimosa spp. development phytomedicines, BP.PD; 14/23951-3 - Standardized phytotherapics for the treatment of chronicle diseases: Rhizophora mangle, BP.MS; 14/26247-5 - Study of Chemical composition of pulp and fiber of Cocos nucifera fruits, BP.IC; + associated scholarships 13/06188-1 - Arrabidaea sp: studies to enable the development of phytomedicines, BP.PD; 13/10708-0 - INVESTIGATION OF THE MECHANISMS OF ACTION OF CRUDE EXTRACT OF BAUHINIA HOLOPHYLLA IN LIVER, SQUELETIC MUSCLE AND ADIPOSE TISSUE OF STREPTOZOTOCIN DIABETIC MICE, BP.DR; 12/18760-9 - Standardized plant extracts for the treatment of chronic diseases: Mimosa spp., BP.DR; 12/22140-6 - Modulating action of Solanum lycocarpum on the carcinogenesis of colon and liver in Wistar rats, BP.PD; 12/23605-2 - Investigation of mechanism involved in hypoglycemic action of crude extract of Myrcia bella in liver, skeletal muscle and adipose tissue in model type 1 diabetes by streptozotocin, BP.DR; 12/50333-3 - Standardized herbal medicines for the treatment of chronic diseases: biomonitoring and standardized chemical studies of Petroselinum crispum (Mill.) Nyman (Apiaceae), BP.PD; 12/05305-1 - Determination of the antiulcerogenic activity of Crotalaria pallida Aiden (FABACEAE), BP.IC; 12/01996-0 - Standardized phytoterapics for the treatment of chronic diseases: Cytotoxic, mutagenic and protective properties and the modulation of gene expression, BP.PD; 11/15801-3 - Gastroprotective Effect, Evaluation of Mechanisms of Antiulcer Action and Healing of Extract and Partitions of Leaves and Fruits of Solanum paniculatum., BP.MS; 11/08880-4 - Standardized extracts for the treatment of chronic diseases, BP.DR; 11/09980-2 - Phytotherapics standard for the treatment of chronic diseases: pharmacognostic quality control, microbiological and pharmacological study of medicinal plants of the Brazilian cerrado., BP.PD; 10/12901-4 - Evaluation of hypoglycemic effect of the extract of Bauhinia holophylla in diabetic mice induced by streptozotocin, BP.MS; 11/03355-9 - Hypoglycemic effetct of Myrcia bella extract in streptozotocin-induced diabetic mice, BP.MS; 11/06529-8 - Phytochemical and pharmacological study of the fruits of Solanum paniculatum (Solanaceae)., BP.IC; 10/09740-9 - Standardized Vegetable extracts for the treatment of chronic diseases: Solanum paniculatum (Solanaceae), BP.PD; 09/17877-7 - Standardized herbal medicines for the treatment of chronic diseases: Arrabidaea spp, BP.DR - associated scholarships

Abstract

Increased life expectancy has increased the demand for drugs useful in the treatment of chronic diseases, especially those affecting the digestive tract (gastric and duodenal ulcers, ulcerative colitis and Crohn's disease), diabetes, cancer and other inflammatory diseases. The use of standardized herbal drugs with efficacy and safety has presented some surprising results. In Brazil, there are deficiencies in the correct characterization of chemical raw materials, as well as the evaluation of pharmacological and toxicological activities of these materials, essential steps to ensure efficacy and safety of herbal medicines. Recently, the Ministry of Health released a list of 71 plants for the development of herbal medicines, however, points out that the listed plant species are still not sufficient to meet the needs of government health plans, including within the National Policy on Medicinal Plants and Phytomedicines. As pointed out by external expertise to our previous project, the integrated chemical and pharmacological approach ‘contributes to the development of new drugs, which is important to reducing the national dependency on the import of drugs and medicine’. Thus, this project proposes to standardize new herbal medicines to treat chronic diseases in the Brazilian population: gastrointestinal (ulcers, colitis, inflammatory bowel disease), inflammation, chronic pain, cancer and diabetes. Extracts will be prepared in accordance with pharmacopoeial standards and evaluated by pharmacological aspects (ulcers, cancer, diarrhea, inflammation, diabetes, immunomodulation) and toxicological (acute and chronic toxicity, genotoxicity). The most promising extracts will be standardized according to international standards. The work will be interdisciplinary, with participants from several campi of UNESP (Institute of Chemistry of Araraquara, Faculty of Pharmaceutical Sciences of Araraquara, Biosciences Institute of Botucatu, College of Agricultural Sciences and Veterinary Medicine of Botucatu, Faculty of Sciences of Assis, Faculty of Science of Bauru, University of Campinas, Universidade Estadual de Londrina, Federal University of Santa Catarina and Universidade Federal de Belo Horizonte), consolidating a network of chemical and pharmacological studies of medicinal plants in Brazil. Our research group has always been focused on chemical and pharmacological study of plants with potential biological activity. The studies include the isolation and identification of substances in plant extracts, accompanied by appropriate pharmacological biomonitoring. This approach led to the study of hundreds of plant extracts of various Brazilian plant species, with large volume of results and significant human resources training, all recorded in their Curriculum Lattes. We have isolated and identified sesquiterpene lactones, alkaloids, flavonoids, catechins, tannins, terpenoids, phenolic acids, nafiopironas, glycolipids and saponins, many with proven anti-ulcer activity, anti-venom serum, anti-inflammatory and healing effect. A patent has been applied along with a national pharmaceutical industry (INPI-PI0401592-4). Therefore, the involvement of a multidisciplinary team encourages wide dissemination of knowledge and complete integrated study of medicinal plants and promotes the optimization of financial resources and ultimately contributes to the country to obtain technological and scientific expertise for greater autonomy in the area of drugs. (AU)

Scientific publications

(46)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BONIFACIO, BRUNA VIDAL; DOS SANTOS RAMOS, MATHEUS APARECIDO; DA SILVA, PATRICIA BENTO; SILVEIRA NEGRI, KAMILA MARIA; LOPES, ERICA DE OLIVEIRA; DE SOUZA, LEONARDO PEREZ; VILEGAS, WAGNER; PAVAN, FERNANDO ROGERIO; CHORILLI, MARLUS; BAUAB, TAIS MARIA. Nanostructured lipid system as a strategy to improve the anti-Candida albicans activity of Astronium sp.. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 10, p. 5081-5092, 2015. (09/52237-9, 13/25121-5, 13/25432-0)

DE SOUZA MESQUITA, LEONARDO MENDES; BITTENCOURT RODRIGUES, CAROLINE FABRI; DA ROCHA, CLAUDIA QUINTINO; BIANCHIM, MAYARA SILVEIRA; RODRIGUES, CLENILSON MARTINS; DE OLIVEIRA, VANDA MARIA; GAETA, HENRIQUE HESSEL; BELCHOR, MARIANA NOVO; TOYAMA, MARCOS HIKARI; VILEGAS, WAGNER. LC-ESI-IT-MS/MS and MALDI-TOF Approach: Identification of Natural Polymers from Rhizophora mangle Barks and Determination of Their Analgesic and Anti-inflammatory Properties. NATURAL PRODUCTS AND BIOPROSPECTING, v. 9, n. 1, p. 23-34, FEB 2019. (14/23951-3, 09/52237-9)

DE SOUZA MESQUITA, LEONARDO MENDES; DELEVATI COLPO, KARINE; DA ROCHA, CLAUDIA QUINTINO; GATTE-PICCHI, DOUGLAS; PEREIRA TANGERINA, MARCELO MARUCCI; ZACHELLO-NUNES, BEATRIZ; GONCALVES MARTINS, MARIA BERNADETE; VILEGAS, WAGNER. Anatomical differentiation and metabolomic profiling: a tool in the diagnostic characterization of some medicinal Plantago species. BRAZILIAN JOURNAL OF BOTANY, v. 40, n. 3, p. 801-810, SEP 2017. (11/23113-0, 09/52237-9)

ROZZA, A. L.; CESAR, D. A. S.; PIERONI, L. G.; SALDANHA, L. L.; DOKKEDAL, A. L.; DE-FARIA, F. M.; SOUZA-BRITO, A. R. M.; VILEGAS, W.; TAKAHIRA, R. K.; PELLIZZON, C. H.. Antiulcerogenic Activity and Toxicity of Bauhinia holophylla Hydroalcoholic Extract. Evidence-based Complementary and Alternative Medicine, 2015. (12/05323-0, 09/52237-9)

MININEL, FRANCISCO JOSE; LEONARDO JUNIOR, CARLOS SERGIO; ESPANHA, LIVIA GREGHI; RESENDE, FLAVIA APARECIDA; VARANDA, ELIANA APARECIDA; FUJIMURA LEITE, CLARICE QUEICO; VILEGAS, WAGNER; DOS SANTOS, LOURDES CAMPANER. Characterization and Quantification of Compounds in the Hydroalcoholic Extract of the Leaves from Terminalia catappa Linn. (Combretaceae) and Their Mutagenic Activity. Evidence-based Complementary and Alternative Medicine, 2014. (09/52237-9)

M.E. GALLON; B.S.P. BARROS; M.A. SILVA; S.H.M. DIAS; G. ALVES-DA-SILVA. Determinação dos parâmetros anatômicos, físico-químico e fitoquímicos das folhas de Solanum lycocarpum A. St.- Hill. Revista Brasileira de Plantas Medicinais, v. 17, n. 4, p. 937-944, 2015-00-00. (09/52237-9)

DA ROCHA, CLAUDIA QUINTINO; DE-FARIA, FELIPE MEIRA; MARCOURT, LAURENCE; EBRAHIMI, SAMAD NEJAD; KITANO, BRUNA TIEMI; GHILARDI, AMANDA FRANCESCHINI; FERREIRA, ANDERSON LUIZ; DE ALMEIDA, ANA CRISTINA ALVES; DUNDER, RICARDO JOSE; MONTEIRO SOUZA-BRITO, ALBA REGINA; et al. Gastroprotective effects of hydroethanolic root extract of Arrabidaea brachypoda: Evidences of cytoprotection and isolation of unusual glycosylated polyphenols. Phytochemistry, v. 135, p. 93-105, MAR 2017. (12/01868-1, 14/03728-8, 09/52237-9)

VALERINO-DIAZ, ALEXANDER B.; GAMIOTEA-TURRO, DAYLIN; ZANATTA, ANA C.; VILEGAS, WAGNER; GOMES MARTINS, CARLOS HENRIQUE; SILVA, THAYNA DE SOUZA; RASTRELLI, LUCA; DOS SANTOS, LOURDES CAMPANER. New Polyhydroxylated Steroidal Saponins from Solanum paniculatum L. Leaf Alcohol Tincture with Antibacterial Activity against Oral Pathogens. Journal of Agricultural and Food Chemistry, v. 66, n. 33, p. 8703-8713, AUG 22 2018. (15/04899-3, 09/52237-9)

PERICO, LARISSA LUCENA; RODRIGUES, VINICIUS PEIXOTO; OHARA, RIE; ALFIERI NUNES, VANIA VASTI; MACHADO DA ROCHA, LUCIA REGINA; VILEGAS, WAGNER; DOS SANTOS, CATARINA; HIRUMA-LIMA, CLELIA AKIKO. Can the gastric healing effect of Eugenia punicifolia be the same in male and female rats?. Journal of Ethnopharmacology, v. 235, p. 268-278, MAY 10 2019. (09/52237-9)

RESENDE, FLAVIA APARECIDA; NOGUEIRA, CATARINE HAIDE; ESPANHA, LIVIA GREGHI; BOLDRIN, PAULA KARINA; OLIVEIRA-HOHNE, ANA PAULA; DE CAMARGO, MARIANA SANTORO; DA ROCHA, CLAUDIA QUINTINO; VILEGAS, WAGNER; VARANDA, ELIANA APARECIDA. In vitro toxicological assessment of Arrabidaea brachypoda (DC.) Bureau: Mutagenicity and estrogenicity studies. REGULATORY TOXICOLOGY AND PHARMACOLOGY, v. 90, p. 29-35, NOV 2017. (09/52237-9)

SPECIAN, ANA FLAVIA L.; SERPELONI, JULIANA M.; TUTTIS, KATIUSKA; RIBEIRO, DIEGO L.; CILIAO, HELOISA L.; VARANDA, ELIANA A.; SANNOMIYA, MIRIAM; MARTINEZ-LOPEZ, WILNER; VILEGAS, WAGNER; COLUS, ILCE M. S.. LDH, proliferation curves and cell cycle analysis are the most suitable assays to identify and characterize new phytotherapeutic compounds. Cytotechnology, v. 68, n. 6, p. 2729-2744, DEC 2016. (09/52237-9)

TUTTIS, KATIUSKA; DA COSTA, DARYNE LU MALDONADO GOMES; SERPELONI, JULIANA MARA; DOS SANTOS, LOURDES CAMPANER; VARANDA, ELIANA APARECIDA; VILEGAS, WAGNER; MARTINEZ-LOPEZ, WILNER; COLUS, ILCE MARA DE SYLLOS. Phytochemical Profile, and Antiproliferative and Proapoptotic Effects ofPouteria ramiflora(Mart.) Radlk. Leaf Extract, and Its Synergism with Cisplatin in HepG2 Cells. JOURNAL OF MEDICINAL FOOD, v. 24, n. 5, p. 452-463, MAY 1 2021. (09/52237-9)

SALDANHA, LUIZ LEONARDO; DELGADO, AISLAN QUINTILIANO; MARCOURT, LAURENCE; DE PAULA CAMAFORTE, NATHALIA APARECIDA; PONCE VAREDA, PRISCILLA MARIA; EBRAHIMI, SAMAD NEJAD; VILEGAS, WAGNER; DOKKEDAL, ANNE LIGIA; QUEIROZ, EMERSON FERREIRA; WOLFENDER, JEAN-LUC; et al. ypoglycemic active principles from the leaves of Bauhinia holophylla: Comprehensive phytochemical characterization and in vivo activity profil. PLoS One, v. 16, n. 9, SEP 24 2021. (16/18628-4, 09/52237-9)

SERPELONI, J. M.; SPECIAN, A. F. L.; RIBEIRO, D. L.; BENICIO, L. M.; NUNES, H. L.; FRANCHI, L. P.; ROCHA, C. Q.; VILEGAS, W.; VARANDA, E. A.; COLUS, I. M. S.. Fridericia platyphylla (Cham.) LG Lohmann root extract exerts cytotoxic and antiproliferative effects on gastric tumor cells and downregulates BCL-XL, BIRC5, and MET genes. HUMAN & EXPERIMENTAL TOXICOLOGY, v. 39, n. 3, NOV 2019. (12/01996-0, 09/52237-9)

VALERINO-DIAZ, ALEXANDER B.; ZANATTA, ANA C.; GAMIOTEA-TURRO, DAYLIN; BOLELA BOVO CANDIDO, ANA CAROLINA; MAGALHAES, LIZANDRA GUIDI; VILEGAS, WAGNER; DOS SANTOS, LOURDES CAMPANER. An enquiry into antileishmanial activity and quantitative analysis of polyhydroxylated steroidal saponins from Solanum paniculatum L. leaves. Journal of Pharmaceutical and Biomedical Analysis, v. 191, NOV 30 2020. (09/52237-9, 16/21044-4)

FRANCO, D. M.; SALDANHA, L. L.; SILVA, E. M.; NOGUEIRA, F. T. S.; DOKKEDAL, A. L.; SANTOS, C.; ROLIM DE ALMEIDA, L. F.. Effects of leaf extracts of Myrcia guianensis (Aubl.) DC. on growth and gene expression during root development of Sorghum bicolor (L.) Moench. ALLELOPATHY JOURNAL, v. 35, n. 2, p. 237-248, APR 2015. (09/52237-9)

SPECIAN, ANA FLAVIA L.; TUTTIS, KATIUSKA; SERPELONI, JULIANA M.; RIBEIRO, DIEGO L.; NUNES, HIGOR LOPES; TANGERINA, MARCELO M. P.; SANNOMIYA, MIRIAM; VARANDA, ELIANA A.; VILEGAS, WAGNER; COLUS, ILCE MARA S.. Chemical characterization of Brazilian savannah Byrsonima species (muricis) and their impact on genomic instability and chemopreventive effects. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v. 887, p. 15-pg., 2023-01-27. (09/52237-9)

DE SOUZA MESQUITA, LEONARDO MENDES; BITTENCOURT RODRIGUES, CAROLINE FABRI; DA ROCHA, CLAUDIA QUINTINO; BIANCHIM, MAYARA SILVEIRA; RODRIGUES, CLENILSON MARTINS; DE OLIVEIRA, VANDA MARIA; GAETA, HENRIQUE HESSEL; BELCHOR, MARIANA NOVO; TOYAMA, MARCOS HIKARI; VILEGAS, WAGNER. LC-ESI-IT-MS/MS and MALDI-TOF Approach: Identification of Natural Polymers from Rhizophora mangle Barks and Determination of Their Analgesic and Anti-inflammatory Properties. NATURAL PRODUCTS AND BIOPROSPECTING, v. 9, n. 1, p. 12-pg., 2019-02-01. (09/52237-9, 14/23951-3)

ROZZA, A. L.; CESAR, D. A. S.; PIERONI, L. G.; SALDANHA, L. L.; DOKKEDAL, A. L.; DE-FARIA, F. M.; SOUZA-BRITO, A. R. M.; VILEGAS, W.; TAKAHIRA, R. K.; PELLIZZON, C. H.. Antiulcerogenic Activity and Toxicity of Bauhinia holophylla Hydroalcoholic Extract. Evidence-based Complementary and Alternative Medicine, v. 2015, p. 9-pg., 2015-01-01. (09/52237-9, 12/05323-0)

MININEL, FRANCISCO JOSE; LEONARDO JUNIOR, CARLOS SERGIO; ESPANHA, LIVIA GREGHI; RESENDE, FLAVIA APARECIDA; VARANDA, ELIANA APARECIDA; FUJIMURA LEITE, CLARICE QUEICO; VILEGAS, WAGNER; DOS SANTOS, LOURDES CAMPANER. Characterization and Quantification of Compounds in the Hydroalcoholic Extract of the Leaves from Terminalia catappa Linn. (Combretaceae) and Their Mutagenic Activity. Evidence-based Complementary and Alternative Medicine, v. 2014, p. 11-pg., 2014-01-01. (09/52237-9)

BONIFACIO, BRUNA VIDAL; DOS SANTOS RAMOS, MATHEUS APARECIDO; DA SILVA, PATRICIA BENTO; SILVEIRA NEGRI, KAMILA MARIA; LOPES, ERICA DE OLIVEIRA; DE SOUZA, LEONARDO PEREZ; VILEGAS, WAGNER; PAVAN, FERNANDO ROGERIO; CHORILLI, MARLUS; BAUAB, TAIS MARIA. Nanostructured lipid system as a strategy to improve the anti-Candida albicans activity of Astronium sp.. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 10, p. 12-pg., 2015-01-01. (09/52237-9, 13/25432-0, 13/25121-5)

SALDANHA, LUIZ L.; VILEGAS, WAGNER; DOKKEDAL, ANNE L.. Characterization of Flavonoids and Phenolic Acids in Myrcia bella Cambess. Using FIA-ESI-IT-MSn and HPLC-PAD-ESI-IT-MS Combined with NMR. Molecules, v. 18, n. 7, p. 8402-8416, JUL 2013. (09/52237-9)

TUTTIS, KATIUSKA; GOMES DA COSTA, DARYNE LU MALDONADO; NUNES, HIGOR LOPES; LEAL SPECIAN, ANA FLAVIA; SERPELONI, JULIANA MARA; DOS SANTOS, LOURDES CAMPANER; VARANDA, ELIANA APARECIDA; VILEGAS, WAGNER; MARTINEZ-LOPEZ, WILNER; DE SYLLOS COLUS, ILCE MARA. Pouteria ramiflora (Mart.) Radlk. extract: Flavonoids quantification and chemopreventive effect on HepG2 cells. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 81, n. 16, p. 792-804, 2018. (09/52237-9)

DIAS SILVA, MARCELO J.; SIMONET, ANA M.; SILVA, NAIARA C.; DIAS, AMANDA L. T.; VILEGAS, WAGNER; MACIAS, FRANCISCO A.. Bioassay-Guided Isolation of Fungistatic Compounds from Mimosa caesalpiniifolia Leaves. Journal of Natural Products, v. 82, n. 6, p. 1496-1502, JUN 2019. (15/21479-8, 12/18760-9, 09/52237-9)

PERICO, LARISSA LUCENA; RODRIGUES, VINICIUS PEIXOTO; OHARA, RIE; BUENO, GABRIELA; ALFIERI NUNES, VANIA VASTI; DOS SANTOS, RAQUEL DE CASSIA; LIMA CAMARGO, ANA CAROLINA; JUSTULIN JUNIOR, LUIS ANTONIO; DE ANDRADE, SERGIO FALONI; BISPO STEIMBACH, VIVIANE MIRANDA; et al. Sex-specific effects of Eugenia punicifolla extract on gastric ulcer healing in rats. WORLD JOURNAL OF GASTROENTEROLOGY, v. 24, n. 38, p. 4369-4383, OCT 14 2018. (09/52237-9, 15/14797-3)

DE SOUZA MESQUITA, LEONARDO MENDES; RABELO E PAIVA CARIA, CINTIA; SANTOS, PAOLA SOUZA; RUY, CAIO CESAR; LIMA, NATALIA DA SILVA; TAKETA MOREIRA, DEBORA KONO; DA ROCHA, CLAUDIA QUINTINO; MURADOR, DANIELLA CARISA; DE ROSSO, VERIDIANA VERA; GAMBERO, ALESSANDRA; et al. Modulatory Effect of Polyphenolic Compounds from the Mangrove Tree Rhizophora mangle L. on Non-Alcoholic Fatty Liver Disease and Insulin Resistance in High-Fat Diet Obese Mice. Molecules, v. 23, n. 9, SEP 2018. (14/23951-3, 09/52237-9)

RODRIGUES, VINICIUS PEIXOTO; DA ROCHA, CLAUDIA QUINTINO; PERICO, LARISSA LUCENA; DOS SANTOS, RAQUEL DE CASSIA; OHARA, RIE; NISHIJIMA, CATARINE MASSUCATO; QUEIROZ, EMERSON FERREIRA; WOLFENDER, JEAN-LUC; MACHADO DA ROCHA, LUCIA REGINA; SOARES SANTOS, ADAIR ROBERTO; et al. Involvement of Opioid System, TRPM8, and ASIC Receptors in Antinociceptive Effect of Arrabidaea brachypoda (DC) Bureau. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 18, n. 11, NOV 2017. (09/52237-9)

DE SOUZA MESQUITA, LEONARDO MENDES; DELEVATI COLPO, KARINE; DA ROCHA, CLAUDIA QUINTINO; GATTE-PICCHI, DOUGLAS; PEREIRA TANGERINA, MARCELO MARUCCI; ZACHELLO-NUNES, BEATRIZ; GONCALVES MARTINS, MARIA BERNADETE; VILEGAS, WAGNER. Anatomical differentiation and metabolomic profiling: a tool in the diagnostic characterization of some medicinal Plantago species. Rev. bras. Bot., v. 40, n. 3, p. 10-pg., 2017-09-01. (11/23113-0, 09/52237-9)

DE SOUZA MESQUITA, LEONARDO MENDES; DA ROCHA, CLAUDIA QUINTINO; LIMA AFFONSO, LUIZ HENRIQUE; CERULLI, ANTONIETTA; PIACENTE, SONIA; PEREIRA TANGERINA, MARCELO MARUCCI; GONCALVES MARTINS, MARIA BERNADETE; VILEGAS, WAGNER. Phenolic Isomers from Plantago catharinea Leaves: Isolation, Identification, Quantification and in vitro Antioxidant Activity. NATURAL PRODUCT COMMUNICATIONS, v. 12, n. 3, p. 409-412, MAR 2017. (09/52237-9, 11/23113-0, 13/07681-3)

BONIFACIO, BRUNA VIDAL; MACHADO VILA, TAISSA VIEIRA; MASIERO, ISADORA FANTACINI; DA SILVA, PATRICIA BENTO; DA SILVA, ISABEL CRISTIANE; LOPES, ERICA DE OLIVEIRA; DOS SANTOS RAMOS, MATHEUS APARECIDO; DE SOUZA, LEONARDO PEREZ; VILEGAS, WAGNER; PAVAN, FERNANDO ROGERIO; et al. Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata. FRONTIERS IN MICROBIOLOGY, v. 10, NOV 15 2019. (13/25432-0, 09/52237-9, 15/19011-8, 13/25121-5)

SANNOMIYA, MIRIAM; RODRIGUES, CLENILSON MARTINS; ARAUJO OLIVEIRA, GIOVANNA CASTRO; SOUZA CARVALHO, JULIANA CAJADO; DA COSTA, LETICIA SERAFIM; SPADARI, CRISTINA DE CASTRO; PENA FERREIRA, MARCELO JOSE; VILEGAS, WAGNER; ISHIDA, KELLY. Galloylquinic acid derivatives from Byrsonima fagifolia leaf extract and potential antifungal activity. Journal of Ethnopharmacology, v. 297, p. 7-pg., 2022-10-28. (09/52237-9, 20/16554-9, 21/01279-5)

PERICO, LARISSA LUCENA; DOS SANTOS, RAQUEL DE CASSIA; RODRIGUES, VINICIUS PEIXOTO; ALFIERI NUNES, VANIA VASTI; VILEGAS, WAGNER; MACHADO DA ROCHA, LUCIA REGINA; DOS SANTOS, CATARINA; HIRUMA-LIMA, CLELIA AKIKO. Role of the antioxidant pathway in the healing of peptic ulcers induced by ischemia-reperfusion in male and female rats treated with Eugenia punicifolia. INFLAMMOPHARMACOLOGY, v. 30, n. 4, p. 12-pg., 2022-04-20. (17/15610-0, 09/52237-9)

SALDANHA, LUIZ LEONARDO; ALLARD, PIERRE-MARIE; AFZAN, ADLIN; DE MELO, FERNANDA PEREIRA DE SOUZA ROSA; MARCOURT, LAURENCE; QUEIROZ, EMERSON FERREIRA; VILEGAS, WAGNER; FURLAN, CLAUDIA MARIA; DOKKEDAL, ANNE LIGIA; WOLFENDER, JEAN-LUC. Metabolomics ofMyrcia bellaPopulations in Brazilian Savanna Reveals Strong Influence of Environmental Factors on Its Specialized Metabolism. Molecules, v. 25, n. 12, JUN 2020. (09/52237-9)

DE ANDRADE, CAROLINA GOMES; DE SOUZA MESQUITA, LEONARDO MENDES; MURADOR, DANIELLA CARISA; CAVALCANTE BRAGA, ANNA RAFAELA; DE ROSSO, VERIDIANA VERA; GARCIA DE ALMEIDA, ODAIR JOSE; VILEGAS, WAGNER. Application of electrospray ionization mass spectrometry fingerprinting associated with macroscopic and histological analysis for Plantago major herbal infusions quality control. Food Research International, v. 107, p. 314-324, MAY 2018. (16/04394-1, 11/23113-0, 09/52237-9)

DOS SANTOS, RAQUEL DE CASSIA; BONAMIN, FLAVIA; PERICO, LARISSA LUCENA; PEIXOTO RODRIGUES, VINICIUS; ZANATTA, ANA CAROLINE; RODRIGUES, CLENILSON MARTINS; SANNOMIYA, MIRIAM; DOS SANTOS RAMOS, MATHEUS APARECIDO; BONIFACIO, BRUNA VIDAL; BAUAB, THIS MARIA; et al. Byrsonima intermedia A. Juss partitions promote gastroprotection against peptic ulcers and improve healing through antioxidant and anti-inflammatory activities. BIOMEDICINE & PHARMACOTHERAPY, v. 111, p. 1112-1123, MAR 2019. (09/52237-9)

DA ROCHA, CLAUDIA QUINTINO; QUEIROZ, EMERSON FERREIRA; MEIRA, CASSIO SANTANA; MAGALHAES MOREIRA, DIOGO RODRIGO; PEREIRA SOARES, MILENA BOTELHO; MARCOURT, LAURENCE; VILEGAS, WAGNER; WOLFENDER, JEAN-LUC. Dimeric Flavonoids from Arrabidaea brachypoda and Assessment of Their Anti-Trypanosoma cruzi Activity. Journal of Natural Products, v. 77, n. 6, p. 1345-1350, JUN 2014. (09/17877-7, 09/52237-9)

BASTING, ROSANNA T.; NISHIJIMA, CATARINE M.; LOPES, JULIANA A.; SANTOS, RAQUEL C.; PERICO, LARISSA LUCENA; LAUFER, STEFAN; BAUER, SILKE; COSTA, MIRIAM F.; SANTOS, LOURDES C.; ROCHA, LUCIA R. M.; et al. Antinociceptive, anti-inflammatory and gastroprotective effects of a hydroalcoholic extract from the leaves of Eugenia punicifolia (Kunth) DC. in rodents. Journal of Ethnopharmacology, v. 157, p. 257-267, NOV 18 2014. (11/20145-8, 09/52237-9)

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Filed patent(s) as a result of this research project