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Modulation of immune response by oral zinc supplementation in adjuvant chemotherapy for colon cancer: a study of global gene expression and function of humoral immunity and neutrophils

Grant number: 10/08787-1
Support type:Regular Research Grants
Duration: November 01, 2010 - August 31, 2013
Field of knowledge:Health Sciences - Medicine - Medical Clinics
Principal Investigator:Selma Freire de Carvalho da Cunha
Grantee:Selma Freire de Carvalho da Cunha
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Assoc. researchers:Camila Bitu Moreno Braga ; Eduardo Magalhães Rego ; Fernanda Maris Peria ; Helio Vannucchi ; Isabel Kinney Ferreira de Miranda Santos


In leukocytes of patients undergoing adjuvant chemotherapy for colon cancer treatment: a) identify genes modulated by oral supplementation of zinc; b) evaluate the effects of oral zinc supplementation on humoral immunity and neutrophil function. The study will be conducted on 30 adult patients aged less than or equal to 65 years, of both genders who have undergone surgical resection of colonic neoplastic lesions without metastatic lesion. Patients will be randomized into two groups, with the first (Group QT Zn, n = 15) receive 70 mg/d of zinc for 24 weeks and the second will receive placebo (QT Placebo Group, n = 15). The study will also include 30 healthy volunteers who receive supplementation of 70 mg/d of Zn (C Zn group, n = 15) or placebo (Group C Placebo, n = 15). Zinc supplementation or placebo for all study groups will start two days before the volunteers received the pneumococcal vaccine, polyvalent 23. Fifteen days after vaccination, patients begin chemotherapy as pre-established criteria by the Oncology Service. Will be monitored the parameters of nutritional status (anthropometry, bioelectrical impedance, food intake, and laboratory tests) adverse effects, according to rules of the CTCAE. In the evaluation of humoral imunity, antibodies opsonization and in the pneumococcal polysaccharide will be measured. Will be evaluated the function of neutrophils by measuring DNA NETs and quantified calprotectin and elastase released in the culture supernatants of activated neutrophils. RT-qPCR will be done of genes differentially expressed (DEGS) on activated leukocytes. In six volunteers from each group will be analyzed global gene expression from RNA extracted from leukocytes by microarray; will be detected and correlated the molecular pathways modulated by zinc by MetaCore software (GeneGo). The DEGS will be validated by RT-qPCR. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MORENO BRAGA, CAMILA BITU; FERREIRA DE MIRANDA SANTOS, ISABEL KINNEY; PALMEIRA, PATRICIA; PERIA, FERNANDA MARIS; DE FIGUEIREDO RIBEIRO, SOFIA MIRANDA; MARTINEZ, EDSON ZANGIACOMI; RIBEIRO DA ROCHA, JOSE JOAQUIM; DE CARVALHO DA CUNHA, SELMA FREIRE. Effect of Zinc Supplementation on Serological Response to Vaccination Against Streptococcus Pneumoniae in Patients Undergoing Chemotherapy for Colorectal Cancer. NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, v. 67, n. 6, p. 926-932, AUG 18 2015. Web of Science Citations: 3.

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