Taxonomic revision, phylogeny and phylogeography of the Hypostomus ancistroides (Ihering, 1911) species complex from the upper Rio Paraná and Rio Ribeira de Iguape Basins (Siluriformes, Loricariidae)

• Grant number: 10/07149-1
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2010
• End date: February 29, 2012
• Field of knowledge: Biological Sciences - Zoology - Taxonomy of Recent Groups
• Principal Investigator: Mario Cesar Cardoso de Pinna
• Grantee: Mario Cesar Cardoso de Pinna
• Host Institution: Museu de Zoologia (MZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Claudio de Oliveira ; Cláudio Henrique Zawadzki ; Pedro Hollanda Carvalho

Abstract

The so-called species complex Hypostomus ancistroides (Ihering 1911) occurs in rivers of the upper Rio Paraná system and in the costal drainage of Rio Ribeira de Iguape. Recent papers on the taxonomy of fishes show evidence of considerable degree of differentiation and endemism among different rivers basins of the upper Rio Paraná system, suggesting an interesting evolutionary history. However, such issues have not yet been studied in depth. The goals of this project are i) to reveal and describe new species of the genus Hypostomus related to the Hypostomus ancistroides species complex, by means of fine-scale phylogenetic analysis and taxonomic revision; ii) to search for natural processes related to the diversification of this species complex. As a step towards those goals, 400 tissue samples, cross-referenced with 1000 whole preserved specimens of Hypostomus ancistroides, were gathered. The sampling so far gathered covers 170 localities from the entire distribution area recognized in literature for the group. This project will be carried out in two phases. First, phylogenetic and phylogeographic analysis will be performed on the basis of nucleotide sequences of the following molecular markers: ATP synthase 6 & 8 (mitochondrial marker) and F-Reticulon-4 (nuclear marker). In this case, phylogenetic results are critical to unveil the different species-level taxa and their geographical distribution. Analyses will be conducted under maximum parsimony and maximum likelihood methods of tree topology estimation. Phylogeographic analysis aim to reveal natural processes linked to the disruption of gene flow and further speciation, either vicariant or from dispersion and colonization. A second major phase of the project is the morphological-taxonomic analysis of the taxa involved in the problem, with the description of new species and the design of an identification key. The availability of an extensive series of molecular samples properly correlated with carefully preserved and accessioned morphological specimens provides a unique opportunity for systematic research. It is expected that the molecular analysis will provide a map of geographically-structured variation which, in turn, will facilitate the search for morphologically-informative variants, thus providing a case for constructive interaction between molecular and morphological data in the study of biodiversity of neotropical fishes. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARVALHO, PEDRO HOLLANDA; QUEIROZ LIMA, SERGIO MAIA; ZAWADZKI, CLAUDIO HENRIQUE; OLIVEIRA, CLAUDIO; DE PINNA, MARIO. Phylogeographic patterns in suckermouth catfish Hypostomus ancistroides (Loricariidae): dispersion, vicariance and species complexity across a Neotropical biogeographic region. MITOCHONDRIAL DNA, v. 27, n. 5, p. 3590-3596, 2016. (10/07149-1)

CARVALHO, PEDRO HOLLANDA; QUEIROZ LIMA, SERGIO MAIA; ZAWADZKI, CLAUDIO HENRIQUE; OLIVEIRA, CLAUDIO; DE PINNA, MARIO. Phylogeographic patterns in suckermouth catfish Hypostomus ancistroides (Loricariidae): dispersion, vicariance and species complexity across a Neotropical biogeographic region. MITOCHONDRIAL DNA PART A, v. 27, n. 5, p. 7-pg., 2016-01-01. (10/07149-1)