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Construction of mutant gene exoz involved in the synthesis of repeating subunits of EPS and the genes phbA, phbB and phbC responsible for the synthesis of PHB in Rhizobium tropici SEMIA 4080

Grant number: 10/11957-6
Support type:Regular Research Grants
Duration: December 01, 2010 - November 30, 2012
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Eliana Gertrudes de Macedo Lemos
Grantee:Eliana Gertrudes de Macedo Lemos
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
Assoc. researchers:Lúcia Maria Carareto Alves ; Manoel Victor Franco Lemos ; Silvana Pompeia Do Val de Moraes

Abstract

Previous work (Proc. FAPESP 01/05720-4 and 05/53170-4) allowed to verify differences in the compositions of exopolysaccharides (EPS) produced by 26 strains of rhizobia patterns, and analyze polysaccharides required for nodulation of beans. The species of nitrogen fixing bacteria belonging to the families Rhizobiaceae and Phyllobacteriaceae synthesize large amounts of EPS. As the production of EPS is inversely proportional to the synthesis of polyhydroxybutyrate (PHB), research related to the production of PHB in different strains of rhizobia are underway in our laboratory, since this is a non-pathogenic bacteria, and was able to accumulate high concentrations of PHB when its cultivation was optimized (Proc. FAPESP 04/03344-3). Studies cited in the literature showed that with the advent of molecular biology techniques, and advances in genome sequencing have allowed a new approach for elucidating gene functions, by evaluating the phenotypes of mutations by obtaining from a specific gene. In this project we intend to build exoZ mutant gene involved in the synthesis of the repeating subunits of EPS and the genes phbA, phbB and phbC responsible for the synthesis of PHB in Rhizobium tropici SEMIA 4080. Thus, research related to genetic modification of this bacterium, both for understanding the biological role of these polymers, such as to analyze production and physicochemical characterizations are the initial steps for the biotechnological use of these biopolymers economically viable, since new techniques of gene manipulation tools are potentially useful in the production of EPS modified and adaptable to specific industrial uses. (AU)

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
KIELAK, ANNA M.; CASTELLANE, TEREZA C. L.; CAMPANHARO, JOAO C.; COLNAGO, LUIZ A.; COSTA, OHANA Y. A.; CORRADI DA SILVA, MARIA L.; VAN VEEN, JOHANNES A.; LEMOS, ELIANA G. M.; KURAMAE, EIKO E. Characterization of novel Acidobacteria exopolysaccharides with potential industrial and ecological applications. SCIENTIFIC REPORTS, v. 7, JAN 24 2017. Web of Science Citations: 8.
LUQUE CASTELLANE, TEREZA CRISTINA; MACHADO BUENO OTOBONI, ALDA MARIA; DE MACEDO LEMOS, ELIANA GERTRUDES. CHARACTERIZATION OF EXOPOLYSACCHARIDES PRODUCED BY RHIZOBIA SPECIES. Revista Brasileira de Ciência do Solo, v. 39, n. 6, p. 1566-1575, NOV-DEC 2015. Web of Science Citations: 11.
MORETTO, CRISTIANE; LUQUE CASTELLANE, TEREZA CRISTINA; LOPES, ERICA MENDES; OMORI, WELLINGTON PINE; SACCO, LAIS POSTAI; DE MACEDO LEMOS, ELIANA GERTRUDES. Chemical and rheological properties of exopolysaccharides produced by four isolates of rhizobia. International Journal of Biological Macromolecules, v. 81, p. 291-298, NOV 2015. Web of Science Citations: 11.
LUQUE CASTELLANE, TEREZA CRISTINA; PERSONA, MICHELLI ROMANOLI; CAMPANHARO, JOAO CARLOS; DE MACEDO LEMOS, ELIANA GERTRUDES. Production of exopolysaccharide from rhizobia with potential biotechnological and bioremediation applications. International Journal of Biological Macromolecules, v. 74, p. 515-522, MAR 2015. Web of Science Citations: 21.

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