Adult stem cells versus induced pluripotent stem cells

• Grant number: 10/51051-6
• Support Opportunities: Research Projects - Thematic Grants
• Start date: February 01, 2011
• End date: January 31, 2016
• Field of knowledge: Biological Sciences - Biology
• Principal Investigator: Irina Kerkis
• Grantee: Irina Kerkis
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Pesquisadores principais: Lygia da Veiga Pereira
• Associated scholarship(s): 14/02768-6 - Immature stem cells of human dental pulp: a new therapeutic strategy for the bone marrow aplasia treatment in animal models, BP.MS; 13/26925-0 - Molecular characterization and functional analysis of simile-neural cells obtained from stem cells from immature dental pulp, BP.MS; 12/14674-0 - Pluripotency analysis of stem cells-like isolated from fetal tissue, BP.IC

Abstract

Recent findings on heterogeneity, flexibility and plasticity of stem cells are challenging the traditional concept of stem cells as pre-programmed entities. The concept of self-organizing systems offers an alternative, more elegant and comprehensive explanation for the experimental data. In recent decades, biological studies of stem cells have evolved a broad spectrum of phenomena, ranging from cellular level to tissue level, suggesting we need to abandon the classical concept of a rigid hierarchy of stem cells development and organization, in order to better understand the dynamic and functional issues involving these cells. This perspective has implications for the characterization of stem cells from specific tissues (for example, on gene expression profiles and patterns of gene regulation and epigenetic changes). The proposed project is composed of seven different sub-projects that aim to deeply investigate the current concept of adult stem cells niche using as a model: human immature dental pulp stem cells, described by our group. In addition, the project proposes to investigate the existence of pluripotent stem cell niche during embryonic and fetal development. We will also analyze how immature dental pulp stem cells will respond to environmental changes when reintroduced in a "strange-niche". Next, we will study the limits of pluripotency of both: mouse immature dental pulp stem cells, which have already been obtained by us, and induced pluripotent cells, originated from these mouse immature dental pulp stem cells by the method of chimeras generation and germ line transmission. Using cellular reprogramming tool, we will analyze morphological diversity that we observed after isolation of induced pluripotent cells clones from a single cell, focusing on genetic and epigenetic mechanisms, which are responsible for this diversity. Using this same tool, we intend to study reprogramming potential of tumorigenic cells by checking the decrease or loss of malignancy and to provide a model to investigate the control of cancer cells. Thus, this project is challenging and will contribute with scientific and technological results of high-impact to the advancement of stem cells biology. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DIAS CAMARA, DIANA APARECIDA; MAMBELLI, LISLEY INATA; PORCACCHIA, ALLAN SAJ; KERKIS, IRINA. Advances and Challenges on Cancer Cells Reprogramming Using Induced Pluripotent Stem Cells Technologies. JOURNAL OF CANCER, v. 7, n. 15, p. 2296-2303, 2016. (10/51051-6)

DIAS CAMARA, DIANA APARECIDA; DE SA JUNIOR, PAULO LUIZ; DE AZEVEDO, RICARDO ALEXANDRE; FIGUEIREDO, CARLOS ROGERIO; ARALDI, RODRIGO PINHEIRO; LEVY, DEBORA; DE SOUZA, DENER MADEIRO; KERKIS, IRINA. Identification of very small cancer stem cells expressing hallmarks of pluripotency in B16F10 melanoma cells and their reoccurrence in B16F10-derived clones. Experimental Cell Research, v. 391, n. 2, JUN 15 2020. (10/51051-6)

ARALDI, RODRIGO PINHEIRO; D'AMELIO, FERNANDA; VIGERELLI, HUGO; DE MELO, THATIANA CORREA; KERKIS, IRINA. Stem Cell-Derived Exosomes as Therapeutic Approach for Neurodegenerative Disorders: From Biology to Biotechnology. CELLS, v. 9, n. 12, DEC 2020. (10/51051-6)

CAMARA, D. A. D.; PORCACCHIA, A. S.; COSTA, A. S.; AZEVEDO, R. A.; KERKIS, I.. Murine melanoma cells incomplete reprogramming using non-viral vector. Cell Proliferation, v. 50, n. 4, AUG 2017. (10/51051-6)

LIZIER, NELSON F.; KERKIS, ALEXANDRE; GOMES, CICERA M.; HEBLING, JOSIMERI; OLIVEIRA, CAMILA F.; CAPLAN, ARNOLD I.; KERKIS, IRINA. Scaling-Up of Dental Pulp Stem Cells Isolated from Multiple Niches. PLoS One, v. 7, n. 6, JUN 29 2012. (10/51051-6)

SANTOS RIZZO ZUTTION, MARILIA SANCHES; DIAS CAMARA, DIANA APARECIDA; DARIOLLI, RAFAEL; TAKIMURA, CELSO; WENCESLAU, CRISTIANE; KERKIS, IRINA. In vitro heterogeneity of porcine adipose tissue-derived stem cells. TISSUE & CELL, v. 58, p. 51-60, JUN 2019. (10/51051-6)