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Molecular studies in neoplasias of children and adolescents: microRNAs signature and functional studies of candidate genes to target therapy

Abstract

Differences in the microRNA (miRs) signature and functional studies of genes associated to cancer could help in diagnosis, prognosis and suggest new insights in important pathways that could be used as potential therapeutic targets in human malignancies. The aim of this project is to identify miR signatures in different pediatric tumors (acute lymphoblastic leukemia, central nervous system tumors and adrenocortical tumors) and to evaluate the differential miR profile in specific subgroups of these malignancies and also to study the function of genes identified in previous group studies, with potential to be used as therapeutic target in new therapeutic protocol design, especially in poor prognosis patients. Functional studies will be made by silencing and/or activation of selected genes by assays of citotoxicity, cell viability and proliferation, apoptosis and cycle cell, and will include the genes SHOC2 and XPO7 in acute lymphoblastic leukemia cell lines, TNFA and AURKB in adrenocortical tumor cell lines, and VEGF and CA12 in astrocytic tumors and medulloblastoma cell lines. Until now the tumor bank of the participant institutions had stored more than 200 acute lymphoblastic leukemia samples, 60 of children adrenocortical tumors, 15 adult adrenocortical tumors, 50 meddulloblastomas, 50 pilocyticc astrocytomas, 50 glioblastomas as well as non-neoplastic control samples (bone marrow, adrenal and brain tissues). (AU)

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DARRIGO JUNIOR, LUIZ GUILHERME; BARONI, MIRELLA; PEIXOTO LIRA, REGIA CAROLINE; TEIXEIRA, SILVIA; FEDATTO, PAOLA FERNANDA; SILVEIRA, VANESSA SILVA; SUAZO, VERIDIANA KILL; VERONEZ, LUCIANA CHAIN; PANEPUCCI, RODRIGO ALEXANDRE; MARCO ANTONIO, DAVID SANTOS; YUNES, JOSE ANDRES; BRANDALISE, SILVIA REGINA; AGUIAR, SIMONE DOS SANTOS; NEDER, LUCIANO; DE OLIVEIRA, RICARDO SANTOS; MACHADO, HELIO RUBENS; CARLOTTI JR, CARLOS GILBERTO; TONE, LUIZ GONZAGA; VALERA, ELVIS TERCI; SCRIDELI, CARLOS ALBERTO. High-throughput microRNA profile in adult and pediatric primary glioblastomas: the role of miR-10b-5p and miR-630 in the tumor aggressiveness. MOLECULAR BIOLOGY REPORTS, v. 47, n. 9 SEP 2020. Web of Science Citations: 0.
DARRIGO JUNIOR, LUIZ GUILHERME; PEIXOTO LIRA, REGIA CAROLINE; FEDATTO, PAOLA FERNANDA; MARCO ANTONIO, DAVID SANTOS; VALERA, ELVIS TERCI; AGUIAR, SIMONE; YUNES, JOSE ANDRES; BRANDALISE, SILVIA REGINA; NEDER, LUCIANO; SAGGIORO, FABIANO PINTO; BECKER, ALINE PAIXAO; DE OLIVEIRA, RICARDO SANTOS; MACHADO, HELIO RUBENS; PANEPUCCI, RODRIGO ALEXANDRE; TONE, LUIZ GONZAGA; SCRIDELI, CARLOS ALBERTO. MicroRNA profile of pediatric pilocytic astrocytomas identifies two tumor-specific signatures when compared to non-neoplastic white matter. JOURNAL OF NEURO-ONCOLOGY, v. 141, n. 2, p. 373-382, JAN 2019. Web of Science Citations: 3.
BORGES, KLEITON S.; ANDRADE, AUGUSTO F.; SILVEIRA, VANESSA S.; MARCO ANTONIO, DAVID S.; VASCONCELOS, ELTON J. R.; ANTONINI, SONIR R. R.; TONE, LUIZ G.; SCRIDELI, CARLOS A. The aurora kinase inhibitor AMG 900 increases apoptosis and induces chemosensitivity to anticancer drugs in the NCI-H295 adrenocortical carcinoma cell line. ANTI-CANCER DRUGS, v. 28, n. 6, p. 634-644, JUL 2017. Web of Science Citations: 8.
PEIXOTO LIRA, REGIA CAROLINE; FEDATTO, PAOLA FERNANDA; MARCO ANTONIO, DAVID SANTOS; LEAL, LETICIA FERRO; MARTINELLI, CARLOS EDUARDO; DE CASTRO, MARGARET; TUCCI, SILVIO; NEDER, LUCIANO; RAMALHO, LEANDRA; SEIDINGER, ANA LUIZA; CARDINALLI, IZILDA; MASTELLARO, MARIA JOSE; YUNES, JOSE ANDRES; BRANDALISE, SILVIA REGINA; TONE, LUIZ GONZAGA; RAUBER ANTONINI, SONIR ROBERTO; SCRIDELI, CARLOS ALBERTO. IGF2 and IGF1R in pediatric adrenocortical tumors: roles in metastasis and steroidogenesis. Endocrine-Related Cancer, v. 23, n. 6, p. 481-493, JUN 2016. Web of Science Citations: 9.
SILVEIRA, VANESSA S.; FREIRE, BRUNO M. R.; BORGES, KLEITON S.; ANDRADE, AUGUSTO F.; CRUZEIRO, GUSTAVO A. V.; SABINO, JOAO PAULO J.; GLASS, MOGENS LESNER; YUNES, JOSE ANDRES; BRANDALISE, SILVIA REGINA; TONE, LUIZ GONZAGA; SCRIDELI, CARLOS ALBERTO. Hypoxia-related gene expression profile in childhood acute lymphoblastic leukemia: prognostic implications. Leukemia & Lymphoma, v. 55, n. 8, p. 1751-1757, AUG 2014. Web of Science Citations: 7.

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