Influence of photodynamic therapy in the genes Bax, Bcl-2, and ErbB2 in mammary carcinogenesis

• Grant number: 11/00731-0
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2011
• End date: March 31, 2013
• Field of knowledge: Biological Sciences - Biophysics - Radiology and Photobiology
• Principal Investigator: Noeme Sousa Rocha
• Grantee: Noeme Sousa Rocha
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• City of the host institution: Botucatu
• Associated researchers: Daisy Maria Favero Salvadori ; Isabelle Ferreira ; Vanderlei Salvador Bagnato

Abstract

Photodynamic therapy (PDT) is a therapeutic modality used for treatment of malignant tumor that induces cell death by necrosis or apoptosis. In vitro and in vivo are carried out to assess the success or failure of therapy, as well as the responses and cellular changes occurred. This study aims to use protocols with different rates of PDT illumination, determine the predominant mechanism of cell death and comparison of real time PCR techniques and immunohistochemistry to detect the expression of genes Bcl-2, erbB-2 and Bax in tumors treated by PDT with different rates of light and untreated. For 28 such animals receive a single dose of DMBA when the manifestation of the neoplasm will be held cytologic and animals will be divided into four groups: G1: control group, G2 treated with PDT using interstitial illumination lighting 50J/cm2 rate; G3 rate 100J/cm2 lighting, lighting G4 150J/cm2 rate. We will study the protein expression of Bcl-2, erbB-2 and Bax by immunohistochemistry and RT-PCR. The data will be evaluated statistically. (AU)