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Proteomic profile of follicular fluid, granulosa cells and embryo culture medium of patients undergoing in vitro fertilization cycles: identification of factors evolved at oocyte quality and embryo development in patients with different infertility etiologies

Grant number: 10/51873-6
Support type:Regular Research Grants
Duration: June 01, 2011 - May 31, 2014
Field of knowledge:Health Sciences - Medicine - Maternal and Child Health
Principal Investigator:Ismael Dale Cotrim Guerreiro da Silva
Grantee:Ismael Dale Cotrim Guerreiro da Silva
Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil


The follicular fluid (FF) is composed by factors produced in follicular cells metabolism and plasma factors selective transferred through follicular barrier. The study of FF composition and granulosa cells (GC) expression can elucidate the ovarian physiology, oocyte and follicular development processes, and also allows the finding of markers for better selection of oocyte and embryo in in vitro fertilization cycles. The proteomics, an extensive protein study, evaluate the expression of proteins in tissue, cells or biological fluids, which permits the overall protein expression characterization, differential expression and knowledge of gene function in the post-genomic era. The mass spectrometry approach has been applied in order to find new protein biomarkers, and has potential for clarify complexes biological profiles and pathologic mechanisms. The goals of this study are evaluate the proteomic profile of FF, GC and embryo medium culture (EMC) of infertile patient with different etiologies and submitted to in vitro fertilization cycles, using mass spectrometry approach. We are going to include FF, GC and EMC of patients with endometriosis grades III and IV, polycystic ovarian syndrome, and premature ovarian failure. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BONETTI, TATIANA C. S.; HADDAD, DEBORA C. M.; DONNINGUES, THAIS S.; ALEGRETTI, JOSE ROBERTO; MOTTA, EDUARDO L. A.; SEELEY, KENT; SILVA, ISMAEL D. C. G. Expressed proteins and activated pathways in conditioned embryo culture media from IVF patients are diverse according to infertility factors. JORNAL BRASILEIRO DE REPRODUCAO ASSISTIDA, v. 23, n. 4, p. 352-360, OCT-DEC 2019. Web of Science Citations: 0.

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