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Control of diarrhea induced by enterotoxigenic Escherichia coli (ETEC) strains: vaccine formulations based on the CFA/I fimbriae, CfaE adhesin, heat-labile toxin (LT), and a new vaccine protection experimental model


The main objective of this project is the development of subunit vaccines administered via mucosal routes aiming the control of infections associated with strains of ETEC, a major etiological agent of infant diarrhea and travelers' diarrhea. The vaccine formulations to be tested will employ the purified CFA/I fimbriae and its subunit adhesive protein, CfaE. Vaccines to be tested will be added with a mucosal adjuvant, a nontoxic derivative of the heat-labile toxin (LT) also produced by ETEC strains. The vaccine formulations will be administered to DBA/2 mice by the oral and nasal routes and the presence of specific antibodies will be measured in serum and secretions (milk and fecal extracts). Protective efficacy of the induced antibodies will be determined by the in vitro neutralization of ETEC CFA/I+ adhesive properties. The work also provides the use of an animal model, based on newborns of the DBA2 lineage, as a correlate of protection afforded by vaccination against ETEC strains derived from human hosts. The results to be obtained should contribute to the development of new acellular vaccine formulations against diarrhea associated with ETEC infections for clinical and/or veterinarian use. (AU)

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(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LUIZ, WILSON B.; RODRIGUES, JULIANA F.; CRABB, JOSEPH H.; SAVARINO, STEPHEN J.; FERREIRA, LUIS C. S.. Maternal Vaccination with a Fimbrial Tip Adhesin and Passive Protection of Neonatal Mice against Lethal Human Enterotoxigenic Escherichia coli Challenge. Infection and Immunity, v. 83, n. 12, p. 4555-4564, . (10/52167-8)

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