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Application of physical-chemical and enzymatic methods in the saccharification of sugar cane bagasse: studies on microorganisms, fermentative processes and hydrolyses methods

Grant number: 10/12624-0
Support type:Research Projects - Thematic Grants
Duration: May 01, 2011 - April 30, 2016
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal researcher:Eleni Gomes
Grantee:Eleni Gomes
Home Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil
Assoc. researchers:Christiane da Costa Carreira Nunes ; Daniela Alonso Bocchini ; Derlene Attili de Angelis ; Fernando Carlos Pagnocca ; Gustavo Orlando Bonilla Rodriguez ; Hamilton Cabral ; Henrique Ferreira ; Ivonete Ávila ; João Cláudio Thoméo ; Lara Durães Sette ; Maria de Lourdes Teixeira de Moraes Polizeli ; Mauricio Boscolo ; Paula Cristina Garcia Manoel Crnkovic ; Paulo Seleghim Júnior ; Roberto da Silva ; Sebastião Roberto Taboga
Associated grant(s):15/00362-5 - Visit of Dr. Andreas Bueck, researcher from Otto von Guericke Universität, Germany, AV.EXT
14/05486-1 - 22nd European Biomass Conference and Exhibition, AR.EXT
14/04821-1 - Visiting professor: mass spectrometry applied to food and bioenergy, AV.EXT
13/02230-3 - 35th Symposium on Biotechnology for Fuels and Chemicals, AR.EXT
Associated scholarship(s):14/25183-3 - Development and scaling-up of packed-bed bioreactors for cellulase production by solid-state fermentation, BP.PD
13/26693-2 - Screening of arabinose assimilating yeasts to use of sugar cane bagasse for production of bioproducts, BP.MS
13/23385-5 - Hydrolysis and fermentation of cellulosic residues aiming the ethanol production, BP.MS
+ associated scholarships 13/01756-1 - Production of cellulolytic enzymes by fungi Trichoderma reesei and Myceliophthora thermophila and application on saccharification of sugar cane bagasse, BP.MS
13/06234-3 - Thermal and structural analysis of sugarcane bagasse pretreated with ozone, ultrasound and microwave for cellulosic ethanol production by enzymatic hydrolysis, BP.MS
12/18769-6 - Assessment of the glucose's effects on beta-glucosidase produced by the fungus Myceliophthora thermophila M.7.7 and the addition of glucose isomerase on this inhibition during the saccharification of sugar cane bagasse, BP.IC
12/14594-7 - Fungi in the pressmud composting: diversity, genomics and biotechnological potential, BP.DR
11/22461-4 - Improved thermal stability of Thermoascus aurantiacus GH10 xylanase, expressed in E. coli, by directed evolution and rational design: Production, purification, biochemical and physico-chemical characterization of the enzyme., BP.DR
12/06237-0 - Isolation of mesophilic and thermophilic fungi from compost piles plant treatment of organic waste municipal and enzymes production, BP.IC
12/00506-9 - Heterologous expression of cellulases from Myceliophthora heterotalica F.2.1.4 in Pichia pastoris with characterization and purification of the enzymes produced, BP.DR
11/07450-6 - Production by lignocellulosic enzymes in solid-state fermentation by Trichoderma specimens for hydrolysis of sugarcane bagasse with microwave pretreatment., BP.DR - associated scholarships


The sugar cane bagasse correspond to 27-30% of plant dry weight and is a waste very available in Brazil considering 600 ton of sugar cane produced in the last year. This material is used as electric energy source, but high quantity is disposable in the industry. The obtainment of reducing sugar from this material is an approach for improvement of the ethanol production. Techniques involving themochemical, chemical and biochemical method for saccharification of sugar cane bagasse have been proposed. However themochemical and chemical methods could release toxic compounds that are inhibitory for yeasts metabolize, resulting in low yield of ethanol production in subsequent fermentative process. The biochemical techniques using fibrolytic enzymes such as cellulose and xilanases have been are used and present advantage as specificity and not produce side compounds. However the low efficiency of the enzymes on sugar cane bagasse hydrolysis suggests that new studies are necessary to understanding of mechanisms that will possibility the increase in the reducing sugar obtainment from enzymatic hydrolysis of sugar cane bagasse. This is the focus of this work which is based to in the pre-treatment of bagasse by physical and physic-chemical methods for disruption of plant wall cell of the bagasse to facility the enzyme activity on cellulose and hemicellulose polymers. Besides, we propose the microscopic studies of the treated material searching for the understanding of the recalcitrance of bagasse to enzyme hydrolysis. Selection, identification of fungi producer of celullases, xilanases and ligninases with high activity on bagasse, isolation and characterization of the gene and development of bioreactor for enzyme production on solid state fermentation are also objectives of this work. (AU)

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Scientific publications (15)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE CASSIA PEREIRA, J.; PAGANINI MARQUES, N.; RODRIGUES, A.; BRITO DE OLIVEIRA, T.; BOSCOLO, M.; DA SILVA, R.; GOMES, E.; BOCCHINI MARTINS, D. A.. Thermophilic fungi as new sources for production of cellulases and xylanases with potential use in sugarcane bagasse saccharification. Journal of Applied Microbiology, v. 118, n. 4, p. 928-939, . (10/12624-0, 14/02080-4)
DE SOUZA MORETTI, MARCIA MARIA; BOCCHINI-MARTINS, DANIELA ALONSO; CARREIRA NUNES, CHRISTIANE DA COSTA; VILLENA, MARIA AREVALO; PERRONE, OLAVO MICALI; DA SILVA, ROBERTO; BOSCOLO, MAURICIO; GOMES, ELENI. Pretreatment of sugarcane bagasse with microwaves irradiation and its effects on the structure and on enzymatic hydrolysis. APPLIED ENERGY, v. 122, p. 189-195, . (10/12624-0, 14/02080-4)
PEREIRA, JOSIANI DE CASSIA; RIBEIRO LEITE, RODRIGO SIMOES; ALVES DO PRADO, HELOIZA FERREIRA; BOCCHINI MARTINS, DANIELA ALONSO; GOMES, ELENI; DA SILVA, ROBERTO. Production and Characterization of beta-glucosidase Obtained by the Solid-State Cultivation of the Thermophilic Fungus Thermomucor indicae-seudaticae N31. Applied Biochemistry and Biotechnology, v. 175, n. 2, p. 723-732, . (10/12624-0, 14/02080-4)
FRASSATTO, PRISCILA APARECIDA CASCIATORI; CASCIATORI, FERNANDA PERPETUA; THOMEO, JOAO CLAUDIO; GOMES, ELENI; BOSCOLO, MAURICIO; DA SILVA, ROBERTO. Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse. SEMINA-CIENCIAS AGRARIAS, v. 41, n. 5, 1, p. 2097-2115, . (17/16482-5, 18/00996-2, 11/07453-5, 13/01756-1, 10/12624-0, 11/21239-6)
BONFA, EMILY COLFERAI; DE SOUZA MORETTI, MARCIA MARIA; GOMES, ELENI; BONILLA-RODRIGUEZ, GUSTAVO ORLANDO. Biochemical characterization of an isolated 50 kDa beta-glucosidase from the thermophilic fungus Myceliophthora thermophila M.7.7. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 13, p. 311-318, . (10/12624-0, 11/23991-7)
MARQUES, NATALIA PAGANINI; PEREIRA, JOSIANI DE CASSIA; GOMES, ELENI; DA SILVA, ROBERTO; ARAUJO, ANGELA REGINA; FERREIRA, HENRIQUE; RODRIGUES, ANDRE; DUSSAN, KELLY JOHANA; BOCCHINI, DANIELA ALONSO. Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasse. INDUSTRIAL CROPS AND PRODUCTS, v. 122, p. 66-75, . (10/12624-0)
PERRONE, OLAVO MICALI; COLOMBARI, FELIPPE MARIANO; ROSSI, JESSIKA SOUZA; SOUZA MORETTI, MARCIA MARIA; BORDIGNON, SIDNEI EMILIO; CARREIRA NUNES, CHRISTIANE DA COSTA; GOMES, ELENI; BOSCOLO, MAURICIO; DA-SILVA, ROBERTO. Ozonolysis combined with ultrasound as a pretreatment of sugarcane bagasse: Effect on the enzymatic saccharification and the physical and chemical characteristics of the substrate. Bioresource Technology, v. 218, p. 69-76, . (10/12624-0, 14/02080-4, 11/06923-8)
DE SOUZA, ANGELICA R.; DE ARAUJO, GABRIELA C.; ZANPHORLIN, LETICIA M.; RULLER, ROBERTO; FRANCO, FERNANDA C.; TORRES, FERNANDO A. G.; MERTENS, JEFFREY A.; BOWMAN, MICHAEL J.; GOMES, ELENI; DA SILVA, ROBERTO. Engineering increased thermostability in the GH-10 endo-1,4-beta-xylanase from Thermoascus aurantiacus CBMAI 756. International Journal of Biological Macromolecules, v. 93, n. A, p. 20-26, . (14/02080-4, 14/04272-8, 10/12624-0, 11/17658-3)
TRINDADE, LUCAS VINICIUS; DESAGIACOMO, CARLA; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES; DE LIMA DAMASIO, ANDRE RICARDO; FURUYAMA LIMA, ALINE MARGARETE; GOMES, ELENI; BONILLA-RODRIGUEZ, GUSTAVO ORLANDO. Biochemical Characterization, Thermal Stability, and Partial Sequence of a Novel Exo-Polygalacturonase from the Thermophilic Fungus Rhizomucor pusillus A13.36 Obtained by Submerged Cultivation. BIOMED RESEARCH INTERNATIONAL, . (10/12624-0, 11/23991-7)
ORDUNA ORTEGA, JULIETH; MORA VARGAS, JORGE ANDRES; METZKER, GUSTAVO; GOMES, ELENI; DA SILVA, ROBERTO; BOSCOLO, MAURICIO. Enhancing the production of the fermentable sugars from sugarcane straw: A new approach to applying alkaline and ozonolysis pretreatments. RENEWABLE ENERGY, v. 164, p. 502-508, . (14/02080-4, 17/09520-8, 15/11588-4, 17/13230-5, 10/12624-0)
ORTEGA, JULIETH ORDUNA; MORA VARGAS, JORGE ANDRES; PERRONE, OLAVO MICALI; METZKER, GUSTAVO; GOMES, ELENI; DA SILVA, ROBERTO; BOSCOLO, MAURICIO. Soaking and ozonolysis pretreatment of sugarcane straw for the production of fermentable sugars. INDUSTRIAL CROPS AND PRODUCTS, v. 145, . (17/13230-5, 10/12624-0, 17/09520-8, 15/11588-4)
OLIVEIRA, D. S.; TELIS-ROMERO, J.; DA-SILVA, R.; FRANCO, C. M. L.. Effect of a Thermoascus aurantiacus thermostable enzyme cocktail on wheat bread qualitiy. Food Chemistry, v. 143, p. 139-146, . (10/12624-0, 14/02080-4, 10/15347-8)
TRAVAINI, RODOLFO; MORALES OTERO, MARIAN DERLY; COCA, MONICA; DA-SILVA, ROBERTO; BOLADO, SILVIA. Sugarcane bagasse ozonolysis pretreatment: Effect on enzymatic digestibility and inhibitory compound formation. Bioresource Technology, v. 133, p. 332-339, . (10/12624-0, 07/08641-4)
DOS SANTOS GOMES, A. C.; CASCIATORI, F. P.; GOMES, E.; DA COSTA CARREIRA NUNES, C.; MORETTI, M. M. S.; THOMEO, J. C.. Growth kinetics ofMyceliophthora thermophilaM.7 center dot 7 in solid-state cultivation. Journal of Applied Microbiology, v. 130, n. 1, . (14/23453-3, 18/00996-2, 10/12624-0)

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