Sepsis and septic shock: functional and morphological changes in the heart. An experimental study in mice

• Grant number: 09/54010-1
• Support Opportunities: Multi-user Equipment Program
• Duration: October 01, 2010 - September 30, 2012
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Helio Cesar Salgado
• Grantee: Helio Cesar Salgado
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Tipo de Equipamento Multiusuário não informado
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

Sepsis produces pathophysiological changes, with marked hypotension, cardiac depression and decreased systemic perfusion, which contributes to multiple organ dysfunction. Although the cause of death in septic shock is probably multifactorial, myocardial dysfunction is a common complication, occurring prior to the shock. Even if studies in humans suggest the absence of ischemic myocardial alterations in sepsis, abnormal microcirculation induces a disturbance in regional blood flow and poor tissue oxygenation, which could cause relative ischemia in various organs, including the heart. The occurrence of microvascular changes is an issue that has been raised recently in our laboratory: the microcirculation might be implicated in the mechanism of myocyte cell injury, with subsequent cardiac dysfunction and death. Therefore, the objective of this project is to use the model of sepsis induced by the technique of cecal ligation and puncture (CLP) in mice to assess functional, structural and morphological characteristics of myocardial. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CELES, MARA R. N.; MALVESTIO, LYGIA M.; SUADICANI, SYLVIA O.; PRADO, CIBELE M.; FIGUEIREDO, MARIA J.; CAMPOS, ERICA C.; FREITAS, ANA C. S.; SPRAY, DAVID C.; TANOWITZ, HERBERT B.; DA SILVA, JOAO S.; et al. Disruption of Calcium Homeostasis in Cardiomyocytes Underlies Cardiac Structural and Functional Changes in Severe Sepsis. PLoS One, v. 8, n. 7, JUL 23 2013. (10/13199-1, 09/17787-8, 10/19216-5, 10/18629-4, 09/54010-1, 09/02942-8)

CAMPOS, ERICA C.; O'CONNELL, JOAO L.; MALVESTIO, LYGIA M.; DIAS ROMANO, MINNA M.; RAMOS, SIMONE G.; CELES, MARA RUBIA N.; PRADO, CIBELE M.; SIMOES, MARCUS V.; ROSSI, MARCOS A.. Calpain-mediated dystrophin disruption may be a potential structural culprit behind chronic doxorubicin-induced cardiomyopathy. European Journal of Pharmacology, v. 670, n. 2-3, p. 541-553, NOV 30 2011. (10/13199-1, 09/17787-8, 10/19216-5, 09/54010-1, 09/02942-8)

PINTO DE MOURA SILVA, GABRIEL APARECIDO; DA SILVA, STEPHANYA COVAS; BEGGIORA, PAMELLA DA SILVA; MATIAS JUNIOR, IVAIR; MENEZES-REIS, RAFAEL; SANTOS, MARCELO VOLPON; MACHADO, HELIO RUBENS; LOPES, LUIZA DA SILVA. Transcranial ultrasonography as a reliable instrument for the measurement of the cerebral ventricles in rats with experimental hydrocephalus: a pilot study. CHILD'S NERVOUS SYSTEM, v. 37, n. 6, FEB 2021. (09/54010-1, 16/11212-7)

DOS SANTOS, DANIELE O.; BLEFARI, VALDECIR; PRADO, FERNANDA P.; SILVA, CARLOS A.; FAZAN, JR., RUBENS; SALGADO, HELIO C.; RAMOS, SIMONE G.; PRADO, CIBELE M.. Reduced expression of adherens and gap junction proteins can have a fundamental role in the development of heart failure following cardiac hypertrophy in rats. Experimental and Molecular Pathology, v. 100, n. 1, p. 167-176, FEB 2016. (09/17787-8, 10/19216-5, 12/09665-2, 09/54010-1, 14/07527-7)

PRADO, CIBELE M.; CELES, MARA R. N.; MALVESTIO, LYGIA M.; CAMPOS, ERICA C.; SILVA, JOAO S.; JELICKS, LINDA A.; TANOWITZ, HERBERT B.; ROSSI, MARCOS A.. Early dystrophin disruption in the pathogenesis of experimental chronic Chagas cardiomyopathy. Microbes and Infection, v. 14, n. 1, p. 59-68, JAN 2012. (10/13199-1, 09/17787-8, 10/19216-5, 10/18629-4, 09/54010-1)

CELES, MARA R. N.; PRADO, CIBELE M.; ROSSI, MARCOS A.. Sepsis: Going to the Heart of the Matter. PATHOBIOLOGY, v. 80, n. 2, p. 70-86, 2013. (10/19216-5, 09/54010-1, 09/17787-8)

PRADO, FERNANDA P.; DOS SANTOS, DANIELE O.; BLEFARI, VALDECIR; SILVA, CARLOS A.; MACHADO, JULIANO; KETTELHUT, ISIS DO CARMO; RAMOS, SIMONE G.; BARUFFI, MARCELO DIAS; SALGADO, HELIO C.; PRADO, CIBELE M.. Early dystrophin loss is coincident with the transition of compensated cardiac hypertrophy to heart failure. PLoS One, v. 12, n. 12, DEC 21 2017. (12/23649-0, 16/21710-4, 09/17787-8, 13/20549-7, 10/19216-5, 09/54010-1)

DAL-SECCO, DANIELA; DALBO, SILVIA; LAUTHERBACH, NATALIA E. S.; GAVA, FABIO N.; CELES, MARA R. N.; BENEDET, PATRICIA O.; SOUZA, ADRIANA H.; AKINAGA, JULIANA; LIMA, VANESSA; SILVA, KATIUSSIA P.; et al. Cardiac hyporesponsiveness in severe sepsis is associated with nitric oxide-dependent activation of G protein receptor kinase. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, v. 313, n. 1, p. H149-H163, JUL 2017. (13/08216-2, 09/54010-1)