Acquisition of a confocal laser scanning microscope for a Multi-User Microscopy Center at São Paulo State University

• Grant number: 09/53990-2
• Support Opportunities: Multi-user Equipment Program
• Duration: September 01, 2010 - June 30, 2015
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Sebastião Roberto Taboga
• Grantee: Sebastião Roberto Taboga
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Tipo de Equipamento Multiusuário não informado
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

Confocal laser scanning microscopy has become an extremely important tool in various areas of biology, medicine, and material sciences. The researchers at our Institute have acquired experience with this technique through collaborations, within Brazil and abroad. This has increased our interest in acquiring the necessary equipment for performing the technique in our multiuser laboratory, established with FAPESP resources a decade ago. The acquisition of a confocal laser scanning microscope for the Professor Celso Abbade Mourão MultiUser Microscopy Center, located on the São José do Rio Preto campus of UNESP, will benefit a number of FAPESPfunded research projects that are currently underway, or were recently concluded, in various areas of knowledge: reproductive cell biology (headed by Professors Sebastião Taboga and Clássius de Oliveira); inflammation biology (headed by Professor Sonia Oliani); cell biology and insect cytogenetics (headed by Professors Maria Tercilia V. A. Oliveira and Mary Itoyama); molecular biology and molecular cytogenetics of cancer (headed by Professor Ana Elizabete Silva); microscopy in food sciences (headed by Professor Célia Landi-Franco); histophysiology of diabetes and obesity (headed by Professors José R. Bosqueiro and Rejane Maira Góes); virology (headed by Professor Paula Rahal); and biodiversity (headed by Professors Orlando Nechi Jr., Luis H. Zanini Branco, and Nelson Bittencourt Jr.). All of these projects are dependent upon the Microscopy Center and will benefit considerably from the acquisition of the equipment requested. Because the assessment by confocal microscopy combines an important component to all research cited above, making it possible to employ immunocytochemical and topochemical techniques in the identification of specific markers, including their colocalization, which is extremely difficult to detect with conventional fluorescence microscopy. We emphasize that some of the researchers, including the General Coordinator, have published articles involving the use of confocal microscopy. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BIANCARDI, MANOEL F.; DOS SANTOS, FERNANDA C. A.; DE CARVALHO, HERNANDES F.; SANCHES, BRUNO D. A.; TABOGA, SEBASTIAO R.. Female prostate: historical, developmental, and morphological perspectives. Cell Biology International, v. 41, n. 11, SI, p. 1174-1183, NOV 2017. (15/03599-6, 09/16789-7, 09/53990-2)

BIANCARDI, MANOEL F.; PEREZ, ANA P. S.; SUZUKI CAIRES, CASSIA REGINA; GOES, REJANE M.; VILAMAIOR, PATRICIA S. L.; SANTOS, FERNANDA C. A.; TABOGA, SEBASTIAO R.. Prenatal exposure to testosterone masculinises the female gerbil and promotes the development of lesions in the prostate (Skene's gland). REPRODUCTION FERTILITY AND DEVELOPMENT, v. 27, n. 7, p. 1000-1011, 2015. (09/53990-2, 09/16789-7)

BIANCARDI, MANOEL F.; PEREZ, ANA P. S.; CAIRES, CASSIA R. S.; FALLEIROS, JR., LUIZ R.; GOES, REJANE M.; VILAMAIOR, PATRICIA S. L.; FREITAS, JR., DIOGENES R.; SANTOS, FERNANDA C. A.; TABOGA, SEBASTIAO R.. Prenatal and pubertal testosterone exposure imprint permanent modifications in the prostate that predispose to the development of lesions in old Mongolian gerbils. ASIAN JOURNAL OF ANDROLOGY, v. 19, n. 2, p. 160-167, MAR-APR 2017. (09/53990-2, 09/16789-7)

FRANCO, CELIA M. L.. Resistant starch: effect on rheology, quality, and staling rate of white wheat bread. JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE, v. 55, n. 11, p. 4578-4588, NOV 2018. (13/17725-8, 09/53990-2)

AZEVEDO SANCHES, BRUNO DOMINGOS; BIANCARDI, MANOEL FRANCISCO; ALCANTARA DOS SANTOS, FERNANDA CRISTINA; GOES, REJANE MAIRA; LEITE VILAMAIOR, PATRICIA SIMONE; TABOGA, SEBASTIAO ROBERTO. Budding Process During the Organogenesis of the Ventral Prostatic Lobe in Mongolian Gerbil. MICROSCOPY RESEARCH AND TECHNIQUE, v. 77, n. 6, p. 458-466, JUN 2014. (09/53990-2, 09/16789-7, 11/20358-1, 11/06335-9)

DOS SANTOS, FERNANDA C. A.; NEGRE, ANA F. P.; RODRIGUEZ, DANIEL A. O.; DE SOUSA, GESSICA C.; RODRIGUES, GIOVANNA A.; SANCHES, BRUNO D. A.; CARVALHO, HERNANDES F.; TABOGA, SEBASTIAO R.; BIANCARDI, MANOEL F.. emale Prostate Development: Morphological Analysis of the Budding Dynami. Microscopy and Microanalysis, v. 28, n. 1, p. 272-280, FEB 2022. (09/53990-2, 11/06335-9, 11/20358-1, 09/16789-7)

FACINA, CAMILA H.; CAMPOS, SILVANA G. P.; GONCALVES, BIANCA F.; GOES, REJANE M.; VILAMAIOR, PATRICIA S. L.; TABOGA, SEBASTIAO R.. Long-term oral exposure to safe dose of bisphenol A in association with high-fat diet stimulate the prostatic lesions in a rodent model for prostate cancer. PROSTATE, v. 78, n. 2, p. 152-163, FEB 1 2018. (09/53990-2, 13/01619-4)

BIANCARDI, MANOEL F.; DOS SANTOS, FERNANDA C. A.; DE CARVALHO, HERNANDES F.; SANCHES, BRUNO D. A.; TABOGA, SEBASTIAO R.. Female prostate: historical, developmental, and morphological perspectives. Cell Biology International, v. 41, n. 11, p. 10-pg., 2017-11-01. (15/03599-6, 09/16789-7, 09/53990-2)